中兽药制剂“三黄连散”抗炎作用的研究

doi:10.12160/j.issn.1672-5190.2020.01.014

畜牧与饲料科学 ›› 2020, Vol. 41 ›› Issue (1): 77-81.doi: 10.12160/j.issn.1672-5190.2020.01.014

中兽药制剂“三黄连散”抗炎作用的研究

张嘉欣¹, 周淑棉¹, 梁万文², 朱瑜¹, 张娅¹, 韦英益¹, 胡庭俊¹

1.广西大学动物科学技术学院,广西南宁 530005;
2.广西壮族自治区水产科学研究院,广西南宁 530022

收稿日期:2019-12-02 出版日期:2020-01-30 发布日期:2020-03-05
通讯作者: 胡庭俊(1965—),男,教授,博士,主要研究方向为兽医药学与毒理学。
作者简介:张嘉欣(1996—),女,硕士研究生,主要研究方向为中兽药药理学。
基金资助:
广西创新驱动发展专项资金课题“罗非鱼链球菌病新型快速诊断技术与三黄连中草药复方制剂研发应用”(桂科AA17204081-1);国家现代农业产业技术体系广西罗非鱼产业创新团队建设专项资金资助课题:罗非鱼链球菌病“三黄连”合剂的研制(nycytxgxcxtd-08-02-02)

Evaluation of Anti-inflammatory Effect of a Traditional Chinese Veterinary Medicine Preparation of 'Sanhuanglian Powder'

ZHANG jia-xin¹, ZHOU Shu-mian¹, LIANG Wan-wen², ZHU Yu¹, ZHANG Ya¹, WEI Ying-yi¹, HU Ting-jun¹

1.College of Animal Science and Technology, Guangxi University, Nanning 530005, China;
2.Guangxi Academy of Fishery Sciences, Nanning 530022, China

Received:2019-12-02 Online:2020-01-30 Published:2020-03-05

摘要/Abstract

摘要： 为明确三黄连散(SHLP)体内和体外抗炎效果,体外试验采用CCK-8法筛选三黄连散对RAW264.7细胞安全浓度,同时建立LPS诱导RAW264.7细胞的体外炎症模型,以评价药物效果;体内试验用二甲苯诱发小鼠急性炎症模型,测定小鼠耳郭肿胀度、脏器指数及小鼠血清TNF-α、IL-1β、IL-6、IL-8和COX-2含量。结果显示,三黄连散对RAW264.7细胞的安全浓度为50 μg/mL;高、中、低剂量的三黄连散能不同程度降低细胞IL-8、TNF-α、IL-β、COX-2的分泌水平(P<0.05,P<0.01);高剂量的三黄连散能极显著降低细胞上清液中NO的分泌水平(P<0.01);高、中、低剂量的三黄连散均可极显著降低模型小鼠耳郭肿胀和血清炎症因子IL-1β、IL-6、IL-8、TNF-α和COX-2分泌水平(P<0.01);不同浓度三黄连散对小鼠脏器系数无显著影响(P>0.05)。综上表明,三黄连散体内外抗炎效果显著。

关键词: 三黄连散, 炎症因子, 抗炎效果

Abstract: The objective of this study was to assess the in vitro and in vivo anti-inflammatory effect of a traditional Chinese veterinary medicine preparation of 'Sanhuanglian Powder (SHLP)'. In in vitro experiment, the toxicity of SHLP on RAW264.7 cells was investigated by CCK-8 method, and the LPS induced inflammation model in RAW264.7 was established to evaluate the anti-inflammatory effect of SHLP. In in vivo experiment, xylene was used to induce acute inflammation model in mice, and the degree of auricle swelling, organ index and serum levels of TNF-α,IL-1β,IL-6,IL-8 and COX-2 were determined. The results showed that the maximum safe concentration of SHLP on RAW264.7 cells was 50 μg/mL. High, medium and low doses of SHLP could inhibit the secretion of IL-8, TNF-α, IL-β and COX-2 in RAW264.7 cells at different degrees (P<0.05 or P< 0.01). High dose of SHLP extremely significantly (P<0.01) reduced the NO secretion level in culture supernatant of RAW264.7 cells. High, medium and low doses of SHLP extremely significantly (P<0.01) inhibited the auricular swelling and down-regulated the secretion of serum inflammatory cytokines of IL-1β, IL-6, IL-8, TNF-α and COX-2. Various concentrations of SHLP had no significant effect on the organ index of the experimental mice (P>0.05). In conclusion, the traditional Chinese veterinary medicine preparation of 'Sanhuanglian Powder' had significant anti-inflammatory effect in vitro and in vivo.

Key words: 'Sanhuanglian Powder', inflammatory cytokines, anti-inflammatory effect

中图分类号:

S853.75

张嘉欣, 周淑棉, 梁万文, 朱瑜, 张娅, 韦英益, 胡庭俊. 中兽药制剂“三黄连散”抗炎作用的研究[J]. 畜牧与饲料科学, 2020, 41(1): 77-81.

ZHANG jia-xin, ZHOU Shu-mian, LIANG Wan-wen, ZHU Yu, ZHANG Ya, WEI Ying-yi, HU Ting-jun. Evaluation of Anti-inflammatory Effect of a Traditional Chinese Veterinary Medicine Preparation of 'Sanhuanglian Powder'[J]. Animal Husbandry and Feed Science, 2020, 41(1): 77-81.

参考文献

[1] 蒋宁,张永祥,杜冠华.新思路·新方法——中药药理学研究与中药新药研发[J].中国药理学与毒理学杂志,2016,30(9):893-909.
[2] 国家药典委员会.中国药典[M].北京:中国医药科技出版社,2010:184.
[3] 刘菊福,卢长安,廖福龙,等.不同产地黄芩提取物主要药效作用的比较[J].中国中医药信息杂志,2001,8(3):8-30.
[4] 卢芳,董培良,陈平平,等.三种热病证候模型最佳造模方法的探索与评价[J].山东中医杂志,2009,28(2):114-116.
[5] 赵咏梅,李发荣,杨建雄,等.连翘苷降血脂及抗氧化作用的实验研究[J].天然产物研究与开发,2005,17(2):157-159.
[6] 胡竟一,雷玲,余悦,等.连翘的抗炎解热作用研究[J].中药药理与临床,2007,23(3):51-52.
[7] 林丽美,王智民,王金华,等.金银花、连翘及银翘药对水煎剂的抗炎、解热作用研究[J].中国中药杂志,2008,34(4):473-475.
[8] 程国强,冯年平,唐琦文,等.黄芩苷对眼科常见病原菌的体外抗菌作用[J].中国医院药学杂志,2001,21(6):347-348.
[9] 王洪玉,苑艺蕾,陈平平,等.黄芩解热抗炎作用有效组分筛选的研究[J].哈尔滨商业大学学报(自然科学版),2016,32(5):542-545.
[10] 王婷婷,张蕻,杨志敏,等.连翘及连翘叶抑菌抗炎活性及其主要化学成分研究[J].中国药物与临床,2019,19(14):2380-2381.
[11] 陶嘉磊,汪受传,陈彦臻,等.中药复方网络药理学研究述评[J].中华中医药杂志,2019,34(9):3903-3907.
[12] 狄斌,冯年平,刘文英.复方双黄连颗粒与黄芩颗粒剂中黄芩苷在大鼠胆汁中代谢的比较研究[J].中国药科大学学报,2004,35(5):51-56.
[13] TSAI Y L,CHANG M C,LIN L D,et al.Stimulation of prostanoids and IL-8 production in human gingival fibroblasts by Porphyromonas gingivalis LPS is associated with MEK/ERK signaling[J].Journal of Dental Sciences,2014,9(2):205.
[14] EDWARDSON D W,BOUDREAU J,MAPLETOFT J,et al.Inflammatory cytokine production in tumor cells upon chemotherapy drug exposure or upon selection for drug resistance[J].PLoS ONE,2017,12(9):e0183662.
[15] JIN J,SUNDARARAJ K P,SAMUVEL D J,et al.Different signaling mechanisms regulating IL-6 expression by LPS between gingival fibroblasts and mononuclear cells: seeking the common target[J].Clinical Immunology,2012,143(2):188-199.
[16] ELMALI N,BAYSAL O,HARMA A,et al.Effects of resveratrol in inflammatory arthritis[J].Inflammation,2007,30(1/2):1-6.
[17] 詹光杰,肖本见,杨年安.延龄草对LPS诱导大鼠肝损伤的保护作用及机制[J].中国应用生理学杂志,2016,32(4):373-377.
[18] GUTTERIDGE J M,HALLIWELL B.Free radicals and antioxidants in the year 2000: A historical look to the future[J].Annals of the New York Academy of Sciences,2010,899(1):136-147.

中兽药制剂“三黄连散”抗炎作用的研究

Evaluation of Anti-inflammatory Effect of a Traditional Chinese Veterinary Medicine Preparation of 'Sanhuanglian Powder'

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	张儒奇, 朱炳旭, 孙金鹏, 王荣, 孙闵. 腹泻型肠易激综合征大鼠炎症因子与肠道微生物的相关性研究[J]. 畜牧与饲料科学, 2023, 44(2): 17-25.
[2]	史鑫锐, 陈滢锴, 陈中婷, 赵尉丹, 江明生, 陈海兰. 鸡血藤总黄酮对环磷酰胺所致小鼠肝损伤的保护作用[J]. 畜牧与饲料科学, 2022, 43(4): 8-13.
[3]	禹方晓, 曹迷霞, 梁万文, 王春会, 王爽, 韦英益, 胡庭俊. 三黄连散预防罗非鱼链球菌病临床应用研究[J]. 畜牧与饲料科学, 2020, 41(3): 86-91.