关键词: 肉苁蓉, 愈伤组织, 细胞悬浮

Abstract: In order to provide technical support for obtaining stable Cistanche deserticola Y.C. Ma resources, the succulent stems were used in this experiment as research materials to establish a stable cell suspension culture system of Cistanche deserticola Y.C. Ma via plant tissue culture technology and cell culture technology by inducing and sub-culturing of callus. The results showed that the best culture system for the callus of Cistanche deserticola Y.C. Ma was determined as follows: MS+PVP 2 000 mg/L + 2,4-D 2.0 mg/L + KT 0.5 mg/L + GA₃ 1.0 mg/L + sucrose 30 g + agar 7.0 g/L, the pH value was 5.8, and the culture conditions were lighting for 12 h/d at temperature of （25±1） ℃. The yellowish callus was a high-yielding cell line. The cell suspension culture system of Cistanche deserticola Y.C. Ma was determined as follows: B5 + GA₃ 2.0 mg/L + 6-BA 0.5 mg/L + 2,4-D 0.2 mg/L + sucrose 30 g/L, the pH value was 5.8, the culture conditions were lighting for 18 h/d at temperature of （25±1） ℃, and the shaker rotation speed was 110 r/min. The maximum value of cell dry weight （12.350 gDW/L） was obtained when the cell inoculation concentration was 4.0 gDW/L, which was the best culture system. This study has established a callus culture system and a stable cell suspension culture system of Cistanche deserticola Y.C. Ma.

Key words: Cistanche deserticola Y.C. Ma, callus, cell suspension