畜牧与饲料科学 ›› 2022, Vol. 43 ›› Issue (1): 119-123.doi: 10.12160/j.issn.1672-5190.2022.01.020

• 动物疾病防控 • 上一篇    下一篇

犊牛源雷氏普罗威登斯菌的分离鉴定及药物敏感性试验

王娜1, 戴伶俐1, 乌云塔娜2, 毕力格3, 赵世华1, 达来宝力格1   

  1. 1.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031;
    2呼伦贝尔市新巴尔虎右旗动物疫病预防控制中心,内蒙古 新巴尔虎右旗 021300;
    3.呼伦贝尔市新巴尔虎右旗农牧业事业发展中心,内蒙古 新巴尔虎右旗 021300
  • 收稿日期:2021-07-13 修回日期:2021-11-02 出版日期:2022-01-30 发布日期:2022-02-10
  • 通讯作者: 达来宝力格(1979—),男,助理研究员,博士,主要研究方向为动物传染病防控。
  • 作者简介:王娜(1989—),女,助理研究员,硕士,主要研究方向为动物传染病防控。
  • 基金资助:
    内蒙古自治区农牧业创新基金项目(2021CXJJM

Isolation,Identification and Antimicrobial Sensitivity Test of Providencia rettgeri from Calf

WANG Na1, DAI Ling-li1, Wuyuntana2, Bilige3, ZHAO Shi-hua1, Dalaibaolige1   

  1. 1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
    2. Animal Disease Prevention and Control Center of New Barag Right Banner of Hulun Buir City,New Barag Right Banner 021300,China;
    3. Agricultural and Animal Husbandry Affair Development Center of New Barag Right Banner of Hulun Buir City,New Barag Right Banner 021300,China
  • Received:2021-07-13 Revised:2021-11-02 Online:2022-01-30 Published:2022-02-10

摘要: [目的] 确定内蒙古呼伦贝尔市新巴尔虎右旗家庭牧场犊牛腹泻的原因,并为该牧场犊牛腹泻的治疗提供药物选择方案。[方法] 无菌采集发病犊牛肛拭子进行细菌培养鉴定及体外药物敏感性试验。[结果] 从腹泻犊牛肛拭子样本分离到1株细菌,经培养后菌落形态一致,通过革兰染色鉴定、生理生化鉴定及16S rDNA鉴定确定该菌为雷氏普罗威登斯菌;该菌对氨曲南、头孢他啶、头孢噻肟、头孢吡肟、头孢曲松、四环素、氯霉素、呋喃妥因8种药物表现为耐药;对氨苄西林、庆大霉素2种药物表现为中度敏感;对头孢西丁、链霉素、哌拉西林、阿米卡星、氧氟沙星、诺氟沙星、左氧氟沙星7种药物表现为敏感。[结论] 该养殖场的犊牛腹泻是由雷氏普罗威登斯菌所致,临床治疗过程中可使用链霉素。

关键词: 雷氏普罗威登斯菌, 犊牛, 分离鉴定

Abstract: [Objective] To determine the causive agent of calf diarrhea in a family livestock farm in New Barag Right Banner of Hulun Buir City, Inner Mongolia, and to provide references for drug selection for treatment of calf diarrhea in this farm. [Method] Diseased calves′ anal swabs were obtained aseptically for bacterial culture, identification and in vitro antimicrobial sensitivity test. [Result] A bacterial strain was isolated from the anal swab samples of the diarrhea calves. The colonies were similar in morphology in the culture. The strain was identified as Providencia rettgeri by Gram′s staining, physiological and biochemical identification, and 16S rDNA sequence analysis. It was resistant to aztreonam, ceftazidime, cefotaxime, cefepime, ceftriaxone, tetracycline, chloramphenicol, and furantoin, moderately sensitive to ampicillin and gentamicin, and sensitive to cefoxitin, streptomycin, piperacillin, amikacin, ofloxacin, norfloxacin, and levofloxacin. [Conclusion] Providencia rettgeri is the causive agent of calf diarrhea in this farm. For clinical treatment, streptomycin is recommended.

Key words: Providencia rettgeri, calf, isolation and identification

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