畜牧与饲料科学 ›› 2022, Vol. 43 ›› Issue (5): 87-92.doi: 10.12160/j.issn.1672-5190.2022.05.014

• 草业科学 • 上一篇    下一篇

NaOH溶液脱绒对白羊草种子活力的影响

王勃,王菲,夏方山,董秋丽   

  1. 山西农业大学草业学院,山西 太谷 030801
  • 收稿日期:2022-06-21 出版日期:2022-09-30 发布日期:2022-09-21
  • 通讯作者: 夏方山(1983—),男,副教授,博士,主要研究方向为草类植物育种与种子科学。
  • 作者简介:王勃(1998—),女,硕士研究生,主要研究方向为草类植物育种与种子科学。
  • 基金资助:
    山西省重点研发计划项目“白羊草新品种选育及其良种扩繁技术研究(201903D221091);山西农业大学“十四五”生物育种工程项目“乡土草种质资源挖掘与新品种选育(YZGC134);内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题“优良乡土草种质资源挖掘与创新利用(2021KF0303)

Effects of Delinting with Sodium Hydroxide Solution on Seed Vigor of Bothriochloa ischaemum

WANG Bo,WANG Fei,XIA Fang-shan,DONG Qiu-li   

  1. College of Grassland Science, Shanxi Agricultural University, Taigu 030801, China
  • Received:2022-06-21 Online:2022-09-30 Published:2022-09-21

摘要:

[目的]探究NaOH溶液脱绒处理对白羊草(Bothriochloa ischaemum)种子活力的影响,为机械化播种提供便利条件。[方法]分别利用浓度为10%、20%、30%、40%、50%(W/V)的NaOH溶液对白羊草种子进行脱绒处理0(CK)、1、5、10、15 min,分析其对发芽率(Gp)、发芽指数(Gi)、平均发芽时间(MGT)、幼苗活力指数(SVI)的影响,筛选白羊草种子NaOH溶液脱绒的最佳条件。[结果]NaOH溶液浓度及处理时间均对白羊草种子活力具有极显著(P<0.01)影响。相同处理时间下,白羊草种子脱绒1~10 min后Gp、Gi随NaOH溶液浓度的增加而增加,脱绒1 min和10 min后SVI随着NaOH溶液浓度的增加而增加,脱绒5 min后SVI随NaOH溶液浓度的增加呈先降低后升高的趋势,脱绒15 min时Gp、Gi和SVI随NaOH溶液浓度的增加呈先升后降的趋势;在20%~50%的NaOH溶液浓度下,脱绒后白羊草种子Gp、Gi和SVI随处理时间的增加呈先升后降的趋势。经NaOH溶液脱绒处理后白羊草种子的MGT均高于CK。[结论]适宜浓度的NaOH溶液浸种能有效脱去白羊草种子表面的绒毛,提高白羊草种子的活力。浓度为50%的NaOH溶液浸种5 min对白羊草种子活力的促进效果最好。

关键词: NaOH溶液, 白羊草, 种子活力, 种子脱绒

Abstract:

[Objective] The aims of the present study were to assess the effects of delinting with sodium hydroxide (NaOH) solution on seed vigor of Bothriochloa ischaemumB. ischaemum), and to provide convenient conditions for its mechanized sowing. [Method] B. ischaemum seeds were delinted with NaOH solution with concentrations of 10%, 20%, 30%, 40% and 50% (W/V) for 0 (CK), 1, 5, 10 and 15 min, respectively. The germination percentage (Gp), germination index(Gi), mean germination time (MGT) and seedlings vigor index (SVI) were analyzed to determine the best condition for delinting of B. ischaemum seeds. [Result] NaOH solution concentrations and treatment time had extremely significant (P<0.01) effects on B. ischaemum seed vigor. Within the same treatment time, Gp and Gi of B. ischaemum seeds elevated with the increase of NaOH solution concentrations after delinting for 1-10 min; SVI of B. ischaemum seeds improved with the increase of NaOH solution concentrations after delinting for 1 and 10 min, and decreased first and then rose with the increase of NaOH solution concentrations after delinting for 5 min; the Gp, Gi and SVI boosted first and then declined with the increase of NaOH solution concentrations after delinting for 15 min. At the concentrations of 20%-50% of NaOH solution, the Gp, Gi and SVI ascended first and then reduced with the extension of treatment time after delinting. The MGT of B. ischaemum seeds delinted with NaOH solution was all higher than that of CK. [Conclusion] Soaking with appropriate concentrations of NaOH solution effectively removes the surface hair of B. ischaemum seeds and improves their vigor. Delinting with NaOH solution at the concentration of 50% for 5 min has the best effect on promoting the vigor of B. ischaemum seeds.

Key words: sodium hydroxide solution, Bothriochloa ischaemum, seed vigor, seed delinting

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