不同盐碱胁迫对白羊草种子萌发及幼苗生长的影响

doi:10.12160/j.issn.1672-5190.2022.03.016

摘要/Abstract

摘要： [目的]探究不同盐碱胁迫对白羊草（Bothriochloa ischaemum）种子萌发及幼苗生长的影响。[方法]在Na⁺浓度为10、30、90、270 mmol/L的NaCl、Na₂SO₄、Na₂CO₃和NaHCO₃胁迫下进行白羊草种子萌发试验,以蒸馏水处理为对照,分析白羊草种子的发芽率、发芽指数、平均发芽时间,以及白羊草幼苗的活力指数、胚根长和胚芽长的变化规律,揭示白羊草种子萌发及幼苗生长对不同盐碱胁迫的响应。[结果]不同盐碱种类、Na⁺浓度及两者的交互作用对白羊草种子发芽率、发芽指数、平均发芽时间、胚根长及胚芽长存在极显著（P<0.01）影响,白羊草种子发芽率、发芽指数、胚根长及胚芽长均随Na⁺浓度的增加呈下降趋势,平均发芽时间则随Na⁺浓度的增加而延长。不同Na⁺浓度对白羊草种子的幼苗活力指数也存在极显著（P<0.01）影响,其幼苗活力指数随Na⁺浓度的增加而降低,但不同盐碱种类对白羊草种子幼苗活力指数的影响仅有显著（P<0.05）差异,而盐碱种类与Na⁺浓度的交互作用对白羊草种子幼苗活力指数的影响无显著（P>0.05）差异。白羊草种子萌发及幼苗生长能力会随Na⁺浓度的升高而下降,且在NaCl和Na₂SO₄胁迫下要高于Na₂CO₃和NaHCO₃胁迫时。[结论]白羊草种子萌发及幼苗生长可耐受Na⁺浓度为10 mmol/L的NaCl、Na₂SO₄、Na₂CO₃和NaHCO₃胁迫,对中性盐的耐受性要高于碱性盐,且盐碱胁迫对白羊草种子胚根生长的影响要大于对胚芽的影响。

关键词: 白羊草, 盐碱胁迫, 种子活力, 种子萌发, 幼苗生长

Abstract: [Objective] To investigate the effects of different saline-alkali stresses on seed germination and seedling growth of Bothriochloa ischaemum. [Method] The germination of Bothriochloa ischaemum seeds was observed under the stresses of NaCl, Na₂SO₄, Na₂CO₃ and NaHCO₃, respectively. For each saline-alkali species, the Na⁺ concentrations were set at 10, 30, 90, and 270 mmol/L. To reveal the response of seed germination and seedling growth of Bothriochloa ischaemum to varied saline-alkali stresses, using distilled water treatment as the control, the variations in germination percentage, germination index, and mean germination time of the seeds were assessed, and the changes in vigor index, radicle length, and plumule length of the seedlings were characterized. [Result] The seed germination percentage, germination index and mean germination time, as well as the seedling radicle length and plumule length of Bothriochloa ischaemum were all extremely significantly （P<0.01） affected by different saline-alkali species, Na⁺ concentrations, and their interaction. As the Na⁺ concentrations increased, the seed germination percentage and germination index as well as seedling radicle length and plumule length dropped, but the mean germination time elevated. Different Na⁺ concentrations extremely significantly （P<0.01） affected the seedling vigor index as well, and the seedling vigor index declined as the Na⁺ concentrations increased. Different saline-alkali species had significant （P<0.05） effects on the seedling vigor index, while the interaction between saline-alkali species and Na⁺ concentrations had no significant （P>0.05） effects on the seedling vigor index. The ability of seed germination and seedling growth declined as the concentrations of Na⁺ increased, and it was higher under NaCl and Na₂SO₄ stresses than under Na₂CO₃ and NaHCO₃ stresses. [Conclusion] The seed germination and seedling growth of Bothriochloa ischaemum exhibited tolerance to the stresses of NaCl, Na₂SO₄, Na₂CO₃, and NaHCO₃ with a Na⁺ concentration of 10 mmol/L. Its tolerance to neutral salt was higher than that to alkaline salt. The effects of saline-alkali stresses on the radicle growth of Bothriochloa ischaemum seedling were greater than that on the plumule growth.

Key words: Bothriochloa ischaemum, saline-alkali stress, seed vigor, seed germination, seedling growth

中图分类号:

S543
S156.4

陈奕霖, 赵萍, 王勃, 毕铭, 阴禹舟, 王聪聪, 夏方山. 不同盐碱胁迫对白羊草种子萌发及幼苗生长的影响[J]. 畜牧与饲料科学, 2022, 43(3): 96-101.

CHEN Yi-lin, ZHAO Ping, WANG Bo, BI Ming, YIN Yu-zhou, WANG Cong-cong, XIA Fang-shan. Effects of Different Saline-alkali Stresses on Seed Germination and Seedling Growth of Bothriochloa ischaemum[J]. Animal Husbandry and Feed Science, 2022, 43(3): 96-101.

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