鞣花酸对饲喂黄曲霉毒素小鼠生长性能、抗氧化功能及肠道屏障功能的影响

doi:10.12160/j.issn.1672-5190.2024.05.001

摘要/Abstract

摘要： [目的]探究鞣花酸对饲喂黄曲霉毒素小鼠生长性能、抗氧化功能及肠道屏障功能的影响。[方法]将30只体重为18~22 g的雌性昆明系小鼠随机分为对照组、黄曲霉毒素组和鞣花酸组,每组10只;对照组小鼠饲喂基础日粮,黄曲霉毒素组小鼠饲喂添加250 μg/kg黄曲霉毒素的基础日粮,鞣花酸组小鼠饲喂添加250 μg/kg黄曲霉毒素和100 mg/kg鞣花酸的基础日粮,试验期为35 d;称量并记录小鼠的试验初体重和试验末体重,计算试验期间增重;试验结束后采用眼球摘除法采集小鼠血液样本,应用ELISA方法检测小鼠血清抗氧化指标总超氧化物歧化酶（T-SOD）活性、总抗氧化能力（T-AOC）、丙二醛（MDA）含量以及肠道屏障功能指标二胺氧化酶（DAO）活性和D-乳酸含量;采集小鼠空肠组织样本,制备石蜡切片,HE染色观察空肠黏膜形态结构、肠绒毛高度及隐窝深度,计算绒隐比。[结果]黄曲霉毒素组小鼠试验末体重、增重均极显著（P<0.01）低于对照组;鞣花酸组小鼠试验末体重与黄曲霉毒素组相比差异不显著（P>0.05）,但增重极显著（P<0.01）高于黄曲霉毒素组。黄曲霉毒素组小鼠的血清T-AOC和T-SOD活性与对照组相比分别显著（P<0.05）和极显著（P<0.01）降低;鞣花酸组小鼠的血清T-AOC与黄曲霉毒素组相比差异不显著（P>0.05）,但血清T-SOD活性与黄曲霉毒素组相比极显著（P<0.01）升高;黄曲霉毒素组小鼠的血清MDA含量极显著（P<0.01）高于对照组,鞣花酸组小鼠的MDA含量极显著（P<0.01）低于黄曲霉毒素组;黄曲霉毒素组小鼠的血清DAO活性及D-乳酸含量极显著（P<0.01）高于对照组,鞣花酸组小鼠的血清DAO活性显著（P<0.05）低于黄曲霉毒素组,血清D-乳酸含量与黄曲霉毒素组相比差异不显著（P>0.05）。对照组小鼠空肠绒毛和隐窝形态整齐,未见异常变化;黄曲霉毒素组小鼠的空肠组织病理损伤严重,肠绒毛排列不整齐、不紧密、间隙变大,并且有断裂现象,肠隐窝的排列也不整齐;黄曲霉毒素组小鼠的空肠绒毛高度和绒隐比与对照组相比极显著（P<0.01）降低,而肠隐窝深度与对照组相比差异不显著（P>0.05）;鞣花酸组小鼠的空肠组织病理损伤程度与黄曲霉毒素组相比有所减轻,空肠黏膜形态基本正常,肠绒毛断裂有所改善,肠隐窝排列较为整齐;鞣花酸组小鼠的肠绒毛高度和绒隐比极显著（P<0.01）高于黄曲霉毒素组,肠隐窝深度与黄曲霉毒素组相比差异不显著（P>0.05）。[结论]鞣花酸能通过抑制体重下降、改善血清抗氧化功能及空肠黏膜屏障功能,缓解黄曲霉毒素对小鼠机体造成的损伤。

关键词: 鞣花酸, 黄曲霉毒素, 小鼠, 生长性能, 抗氧化, 肠道屏障

Abstract: [Objective] This study was conducted to assess the effects of ellagic acid on growth performance, antioxidant function and intestinal barrier function in mice fed aflatoxin-containing diets. [Method] A total of 30 healthy female Kunming mice weighted 18 to 22 g were randomly assigned into a control group, a toxin group or an ellagic acid treatment group, with 10 mice in each group. The control group fed a basal diet, the toxin group fed the basal diet containing 250 μg/kg aflatoxin, and the ellagic acid treatment group fed the basal diet supplemented with 250 μg/kg aflatoxin and 100 mg/kg ellagic acid. The feeding experiment lasted for 35 days. The mice were individually weighed at the beginning and end of the experiment, and the weight gain were calculated. At the end of the experiment, the blood samples of the mice were collected with ophthalmectomy. The serum antioxidant indexes such as total superoxide dismutase （T-SOD） activity, total antioxidant capacity （T-AOC） and malondialdehyde （MDA） content as well as the intestinal barrier function indexes such as diamine oxidase （DAO） activity and D-lactic acid content were determined by using ELISA assay. The jejunal tissue samples of the mice were collected to prepare paraffin section. The morphological structure of jejunal mucosa, jejunal villus height and jejunal crypt depth were observed with HE staining, and the ratio of villus height to crypt depth was calculated. [Result] The final body weight and the weight gain of toxin group were extremely significantly （P<0.01） lower than control group. While the final body weight of ellagic acid treatment group was not significantly （P>0.05） different from toxin group, the weight gain of ellagic acid treatment group was extremely significantly （P<0.01） higher than toxin group. Toxin group had significantly （P<0.05） decreased serum T-AOC activity and extremely significantly （P<0.01） reduced serum T-SOD activity in comparison to toxin group. Ellagic acid treatment group had extremely significantly （P<0.01） elevated serum T-SOD activity compared with toxin group, while no significant （P>0.05） difference in serum T-AOC activity was found between these two groups. An extremely significantly （P<0.01） higher serum MDA content was observed in toxin group than control group, and an extremely significantly （P<0.01） lower serum MDA content was observed in ellagic acid treatment group than toxin group. Toxin group had extremely significantly （P<0.01） higher serum DAO activity and D-lactic acid content compared with control group. Ellagic acid treatment group had significantly （P<0.05） reduced serum DAO activity compared with toxin group,while no significant （P>0.05） difference in serum D-lactic acid content was found between these two groups. The jejunal villi and crypt of the mice in control group exhibited complete morphology, and no abnormal pathological changes were observed. Serious pathological damages were observed in jejunal tissues of the mice in toxin group：the arrangement of jejunal villi was neither neat nor tight, the intervillous space enlarged and even break, and the arrangement of jejunal crypt was also not neat. The jejunal villus height and the ratio of villus height to crypt depth of toxin group was extremely significantly （P<0.01） decreased compared with control group, while no significant （P>0.05） difference in jejunal crypt depth was found between these two groups. The mice in ellagic acid treatment group exhibited alleviated pathological damages in jejunal tissues compared with toxin group：the morphology of jejunal mucosa was basically restored to normal, the villi breakage was ameliorated, and the jejunal crypt arranged more neatly. The ellagic acid treatment group had extremely significantly （P<0.01） increased jejunal villus height and the ratio of villus height to crypt depth than toxin group, while no significant （P>0.05） difference in jejunal crypt depth was found between these two groups. [Conclusion] Ellagic acid alleviates the damages induced by aflatoxin in mice via inhibiting the body weight reduction, enhancing serum antioxidant function and improving jejunal mucosa barrier function.

Key words: ellagic acid, aflatoxin, mice, growth performance, anti-oxidation, intestinal barrier

中图分类号:

S816.71

李坤, 邓文茜, 葛昆陇, 任德刚, 陈跃明, 秦顺义. 鞣花酸对饲喂黄曲霉毒素小鼠生长性能、抗氧化功能及肠道屏障功能的影响[J]. 畜牧与饲料科学, 2024, 45(5): 1-6.

LI Kun, DENG Wenxi, GE Kunlong, REN Degang, CHEN Yueming, QIN Shunyi. Effects of Ellagic Acid on Growth Performance, Antioxidant Function and Intestinal Barrier Function in Mice Fed Aflatoxin-containing Diets[J]. Animal Husbandry and Feed Science, 2024, 45(5): 1-6.

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