Abstract: This experiment was designed to study the effect of crude extracts from Herba E.humifusae on antioxidant index of mice with spleen-deficiency.The modle was set up to produce splenasthenic syndrome by adopts lapactic and exhaustion for 60 days,the established modle was ensured by determining the activity of GSH-PX and amylase in plasma,when they significantly decline（P〈0.05）or（P〈0.01）compared with that of the former,then mice which is spleen-deficiency were randomly divided into the group of spleen-deficiency（SD） and the group of E.humifusae（EH）,and the control group（C） also was settled.The animals of EH was given decoction of 30 g/（kg·bw）,while the same dosage of sodium chloride in SD and C.60 days later,the trial animals were treated,the content of SOD and MDA was detected.The result showed that SOD activity in SD and EH is markedly lower than that of C（P〈0.05）,with the elongation of using crude extracts from Herba E.humifusae,SOD activity was markedly higher than that of SD（P〈0.05）;Meanwhile,MDA activity in SD and EH is markedly higher than that of C（P〈0.05）,with the elongation of using crude extracts from Herba E.humifusae,MDA activity was markedly higher than that of EH（P〈0.05）.The conclusion could be made that the crude extracts of Herba E.humifusae can enhanced the anti-oxidation activity obviously.