Abstract: Laboratory animals are important subjects and tools for teaching and research in colleges and universities, which is the necessary link of translational medicine. With the innovative development of science and technology, the species and strains of experimental animals used in colleges and universities have been remarkably increased. The emergence of biosafety incidents caused by inappropriate management of laboratory animals has become increasingly frequent,which poses a serious threat to human health and ecological environment. We pointed out the potential biosafety hazard brought by experimental animals in colleges and universities, analyzed the problems existing in biosafety control of experiment animals, and proposed some measures and advices on copping with biosafety hidden danger of laboratory animals.