关键词: 猪瘟病毒, 猪伪狂犬病病毒, 猪繁殖与呼吸综合征病毒, 猪圆环病毒Ⅱ型;, 酶联免疫吸附试验

Abstract: The objectives of this study were to understand the prevalence of several viral infectious diseases with clinical importance in large-scale pig farms near Liaocheng, Shandong Province, including porcine reproductive and respiratory syndrome （PRRS）, porcine epidemic diarrhea （PED）, classical swine fever （CSF）, porcine circovirus type Ⅱ （PCV-2） infection and porcine pseudorabies （PR）, and to evaluate the antibody protection level against the infections caused by these viral pathogens. Enzyme-linked immunosorbent assay （ELISA） was used to detect and analyze the blood samples from 6 large-scale pig farms near Liaocheng. The results showed that the overall antibody positive rates of PCV-2, porcine reproductive and respiratory syndrome virus （PRRSV）, classical swine fever virus （CSFV）, pseudorabies virus-gB （PRV-gB）, and PRV-gE were 69.64%, 87.76%, 74.17%, 87.10% and 77.04%, respectively. Furthermore, the antibody positive rates of PCV-2, PRRSV, CSFV and PRV-gB exhibited an upward trend compared with the previous years, indicating that the pig farms had begun to attach importance to the vaccine prevention. However, the positive rate of PRV-gE has reached up to over 70%, which tells a potential risk of wild-type PRV infection and attention should be constantly given.

Key words: CSFV, PRV, PRRSV, PCV-2, ELISA