广西壮族自治区1株类NADC30猪繁殖与呼吸综合征病毒（PRRSV）GP5基因的遗传变异分析

doi:10.12160/j.issn.1672-5190.2024.03.014

畜牧与饲料科学 ›› 2024, Vol. 45 ›› Issue (3): 100-106.doi: 10.12160/j.issn.1672-5190.2024.03.014

广西壮族自治区1株类NADC30猪繁殖与呼吸综合征病毒（PRRSV）GP5基因的遗传变异分析

周明旭¹, 黄张玲², 黄胜斌², 周庆安², 胡丽萍², 莫胜兰², 韩银华², 蓝惠华², 颜健华³, 韦海娜², 熊毅², 何奇松²

1.广西农业职业技术大学,广西南宁 530007;
2.广西壮族自治区动物疫病预防控制中心,广西南宁 530001;
3.广西大学医学院,广西南宁 530004

收稿日期:2024-03-26 出版日期:2024-05-30 发布日期:2024-06-25
通讯作者: 何奇松（1986—）,男,高级兽医师,硕士,主要研究方向为动物传染病与分子病毒学。
作者简介:周明旭（1978—）,男,兽医师,主要研究方向为动物防疫及动物卫生监督管理。
基金资助:
国家自然科学基金项目（31660713）; 广西壮族自治区农业农村厅科技项目（Z202034）

Genetic Variation Analysis of GP5 Gene of an NADC30-like Strain of Porcine Reproductive and Respiratory Syndrome Virus from Guangxi Zhuang Autonomous Region

ZHOU Mingxu¹, HUANG Zhangling², HUANG Shengbin², ZHOU Qing′an², HU Liping², MO Shenglan², HAN Yinhua², LAN Huihua², YAN Jianhua³, WEI Haina², XIONG Yi², HE Qisong²

1. Guangxi Vocational University of Agriculture,Nanning 530007,China;
2. Guangxi Center for Animal Disease Prevention and Control,Nanning 530001,China;
3. Medical College,Guangxi University,Nanning 530004,China

Received:2024-03-26 Online:2024-05-30 Published:2024-06-25

摘要/Abstract

摘要： [目的]了解广西壮族自治区猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus,PRRSV）的分子流行病学特征并揭示其遗传变异情况。[方法]利用PRRSV实时荧光RT-PCR试剂盒（通用型）对采集自广西壮族自治区某猪场临床发病猪只的肺脏组织样本进行PRRSV检测;利用PRRSV美洲型经典株与类NADC30 PRRSV毒株双重实时荧光RT-PCR试剂盒对样本进行进一步鉴定;采用PCR法扩增GP5基因,将测序获得的核苷酸序列与GenBank数据库中下载的国内外PRRSV参考株GP5基因核苷酸序列进行同源性比对,并构建系统遗传进化树;对GP5基因推导的氨基酸序列进行比对分析。[结果]经鉴定,该份样本呈类NADC30 PRRSV阳性,命名为GXYL株;GP5基因核苷酸序列同源性分析显示,GXYL株与国内外PRRSV参考株同源性为61.9%~93.2%,与美国NADC30毒株的同源性最高,与欧洲型LV毒株的同源性最低;遗传进化树分析显示,GXYL株与美国NADC30毒株以及中国类NADC30毒株位于同一分支,亲缘关系较近,与其他美洲型毒株同处一个大的分支;GP5基因推导的氨基酸比对分析显示,GXYL株共有7个位点发生变异,其中,2个突变位点位于A表位处,1个突变位点位于B表位处,4个突变位点位于信号肽区域,未发现有氨基酸的插入及缺失。[结论]GXYL株为类NADC30 PRRSV毒株,提示该地区应加强对PRRSV流行及变异情况的监控。

关键词: 猪繁殖与呼吸综合征病毒, GP5基因, 遗传变异分析

Abstract: [Objective] The aim of the present study was to characterize the molecular epidemiology and genetic variation of porcine reproductive and respiratory syndrome virus （PRRSV） in Guangxi Zhuang Autonomous Region. [Method] A real-time fluorescent RT-PCR kit for universal detection of PRRSV was utilized to detect the presence of PRRSV in a lung tissue sample collected from the clinically infected pig on a farm in Guangxi Zhuang Autonomous Region. Subsequently, a dual real-time fluorescent RT-PCR kit specific for the PRRSV American classic strain and NADC30-like PRRSV strain were employed to further identify the sample. The GP5 gene was amplified by PCR, and a homologous comparison was conducted between the obtained nucleotide sequence by sequencing and the GP5 gene sequences of both domestic and international PRRSV reference strains retrieved from the GenBank database. Additionally, a phylogenetic tree was constructed. The amino acid sequences derived from the GP5 gene were analyzed by sequence alignment. [Result] The sample was identified as NADC30-like PRRSV positive and designated as GXYL strain. Homologous analysis of the GP5 gene nucleotide sequences indicated that GXYL strain exhibited 61.9% to 93.2% similarity with both domestic and international PRRSV reference strains, sharing the highest homology with PRRSV NADC30 strain from the United States and the lowest homology with PRRSV LV strain of European type. Furthermore, phylogenetic tree analysis based on GP5 gene sequences placed GXYL strain within a branch alongside NADC30-like strains from China and NADC30 strains from the United States, indicating close relatedness among them within a larger branch containing other American type strains. Amino acid comparison analysis derived from GP5 gene sequences identified 7 mutation sites within GXYL strain, among which 2 sites were located at epitope A, 1 site was located at epitope B, and 4 sites were located at signal peptide region. No amino acid insertions or deletions were found. [Conclusion] GXYL strain is a NADC30-like PRRSV strain, suggesting that the surveillance of prevalence and genetic variation in PRRSV should be strengthened in this region.

Key words: porcine reproductive and respiratory syndrome virus, GP5 gene, genetic variation analysis

中图分类号:

S852.659.6

周明旭, 黄张玲, 黄胜斌, 周庆安, 胡丽萍, 莫胜兰, 韩银华, 蓝惠华, 颜健华, 韦海娜, 熊毅, 何奇松. 广西壮族自治区1株类NADC30猪繁殖与呼吸综合征病毒（PRRSV）GP5基因的遗传变异分析[J]. 畜牧与饲料科学, 2024, 45(3): 100-106.

ZHOU Mingxu, HUANG Zhangling, HUANG Shengbin, ZHOU Qing′an, HU Liping, MO Shenglan, HAN Yinhua, LAN Huihua, YAN Jianhua, WEI Haina, XIONG Yi, HE Qisong. Genetic Variation Analysis of GP5 Gene of an NADC30-like Strain of Porcine Reproductive and Respiratory Syndrome Virus from Guangxi Zhuang Autonomous Region[J]. Animal Husbandry and Feed Science, 2024, 45(3): 100-106.

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广西壮族自治区1株类NADC30猪繁殖与呼吸综合征病毒（PRRSV）GP5基因的遗传变异分析

Genetic Variation Analysis of GP5 Gene of an NADC30-like Strain of Porcine Reproductive and Respiratory Syndrome Virus from Guangxi Zhuang Autonomous Region

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