结石通片对小鼠的急性毒性试验及其对乙二酰胺致大鼠肾结石的作用研究

doi:10.12160/j.issn.1672-5190.2021.02.002

摘要/Abstract

摘要： [目的] 研究结石通片对小鼠的急性毒性作用,对健康大鼠的利尿作用,以及对乙二酰胺致大鼠肾结石的调节作用。[方法] 取ICR小鼠40只,雌、雄各半,随机分为对照组与给药组,每组20只,观察结石通片给药前后小鼠的体重变化和死亡情况;另取SD大鼠36只,雌、雄各半,分为对照组、低剂量组（给予5%结石通片溶液）和高剂量组（给予10%结石通片溶液）,统计各组大鼠24 h排尿量;并采用42只雄性SD大鼠,考查结石通片对给予乙二酰胺（2%掺喂）后大鼠体重、肾重和肾重比的影响。[结果] 给予结石通片溶液后,连续观察7 d,小鼠的各项生理活动未见明显异常,给药组与对照组相比体重差异不显著（P>0.05）;与对照组比较,给予结石通片溶液后大鼠24 h内排尿量明显增加（P<0.01）;与对照组比较,给药组随着给药剂量的增加,结晶和结石的数量极显著（P<0.01或P<0.001）减少,形成结石的动物数量极显著（P<0.01或P<0.001）减少。[结论] 结石通片对小鼠无明显急性毒性作用,对大鼠利尿作用明显,并对乙二酰胺致大鼠肾结石具有明显抑制作用。

关键词: 结石通片, 急性毒性作用, 小鼠, 乙二酰胺, 肾结石, 大鼠

Abstract: [Objective] To evaluate the acute toxicity of Jieshitong tablet in mice, to observe its diuretic effect on healthy rats, and to assess its alleviating effect on oxalamide-induced renal calculi in rats. [Method] A total of 40 ICR mice （half females and half males） were selected and randomly divided into a control group and an administration group with 20 mice in each group. The weight changes and deaths of the mice before and after the administration of Jieshitong tablet were observed. In addition, a total of 36 SD rats （half females and half males） were divided into normal saline group （n=12）, low-dose administration group （n=12, 5% Jieshitong tablet solution） and high-dose administration group （n=12, 10% Jieshitong tablet solution）. The 24-hour urine output of the rats in each group was counted. Another 42 male SD rats were used to investigate the effect of Jieshitong tablet on body weights, kidney weights and kidney to weight ratio of rats after treatment of oxalamide （2% mixed feeding）. [Result] During the 7 consecutive days after the administration of Jieshitong tablet solution, there was no obvious abnormality in the physiological activities of the mice, and no significant （P>0.05） difference in body weights between the control group and the administration group was observed. Compared with the normal saline group, the urinary volumes of the two administration groups were significantly （P<0.01) increased within 24 hours after administration of Jieshitong tablet solution. Compared with the oxalamide-induced renal calculi model group, the number of crystals and calculi in the Jieshitong tablet administration groups was significantly （P<0.01 or P<0.001） reduced with the increase of administration dose, and the number of rats that formed renal calculi was significantly （P<0.01 or P<0.001） reduced. [Conclusion] Jieshitong tablet has no obvious acute toxicity to mice, has obvious inhibitive effect in rats, and has obvious inhibitive effect on oxalamide-induced renal calculi in rats.

Key words: Jieshitong tablet, acute toxicity, mice, oxalamide, renal calculi, rats

中图分类号:

R277.5
R983

黎伟华, 金学琴, 吴璟. 结石通片对小鼠的急性毒性试验及其对乙二酰胺致大鼠肾结石的作用研究[J]. 畜牧与饲料科学, 2021, 42(2): 7-10.

LI Wei-hua, JIN Xue-qin, WU Jing. Acute Toxicity Test of Jieshitong Tablet in Mice and Its Effects on Oxalamide-induced Renal Calculi in Rats[J]. Animal Husbandry and Feed Science, 2021, 42(2): 7-10.

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