大庆市某牧场牛白血病病毒感染情况调查

doi:10.12160/j.issn.1672-5190.2021.03.019

畜牧与饲料科学 ›› 2021, Vol. 42 ›› Issue (3): 102-106.doi: 10.12160/j.issn.1672-5190.2021.03.019

大庆市某牧场牛白血病病毒感染情况调查

何思锐^1,2, 武瑞^1,2, 连帅^1,2, 王建发^1,2, 张迪^1,2, 高丽^1,2

1.黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319;
2.黑龙江省牛病防制重点实验室,黑龙江大庆 163319

收稿日期:2021-02-26 出版日期:2021-05-30 发布日期:2021-06-21
通讯作者: 武瑞（1967—）,男,教授,博士,博士生导师,主要研究方向为动物疾病诊断和新兽药研发。
作者简介:何思锐（1997—）,女,硕士研究生,主要研究方向为动物疾病诊断和新兽药研发。
基金资助:
国家自然科学基金项目（31972747,32002247）; 黑龙江优秀青年基金项目（YQ2019C014）

Investigation of Bovine Leukemia Virus Infection at a Dairy Farm in Daqing City

HE Si-rui^1,2, WU Rui^1,2, LIAN Shuai^1,2, WANG Jian-fa^1,2, ZHANG Di^1,2, GAO Li^1,2

1. College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China;
2. Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, Daqing 163319,China

Received:2021-02-26 Online:2021-05-30 Published:2021-06-21

摘要/Abstract

摘要： [目的]了解大庆市某牧场牛白血病病毒（BLV）感染情况。[方法]随机选取大庆市某规模化牧场泌乳期奶牛107头,采用ELISA方法进行BLV血清流行病学调查,并采用荧光定量PCR方法加以验证。[结果]ELISA检测结果阳性率为31.78%（34/107）,荧光定量PCR检测结果阳性率为37.38%（40/107）,两种检验方法的阳性重合率为85.00%;BLV高载量牛占总数的26.17%,低载量牛占总数的11.21%。[结论]调查结果表明,该牧场奶牛存在一定程度的BLV感染,在牛群中传播的风险较高。

关键词: 奶牛, 牛白血病病毒, 酶联免疫吸附试验, 荧光定量PCR, 流行病学调查

Abstract: [Objective] To understand the infection status of bovine leukemia virus （BLV） at a dairy farm in Daqing City. [Method] Blood samples were collected from 107 randomly selected lactating dairy cows. The serological epidemiology investigation of BLV was carried out by ELISA, and was verified by fluorescent quantitative PCR. [Result] The BLV positive rate of ELISA was 31.78% （34/107）, and that of fluorescent quantitative PCR was 37.38% （40/107）. The coincidence rate of positive results of the two test methods was 85.00%. The dairy cows with high BLV load accounted for 26.17% of the total, and those with low BLV load accounted for 11.21% of the total. [Conclusion] The BLV infection is prevalent at a certain level at the dairy farm, and there is a higher risk of BLV spread among the dairy cows in this herd.

Key words: dairy cow, bovine leukemia virus, ELISA, fluorescent quantitative PCR, epidemiological investigation

中图分类号:

S858.235.3

何思锐, 武瑞, 连帅, 王建发, 张迪, 高丽. 大庆市某牧场牛白血病病毒感染情况调查[J]. 畜牧与饲料科学, 2021, 42(3): 102-106.

HE Si-rui, WU Rui, LIAN Shuai, WANG Jian-fa, ZHANG Di, GAO Li. Investigation of Bovine Leukemia Virus Infection at a Dairy Farm in Daqing City[J]. Animal Husbandry and Feed Science, 2021, 42(3): 102-106.

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大庆市某牧场牛白血病病毒感染情况调查

Investigation of Bovine Leukemia Virus Infection at a Dairy Farm in Daqing City

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