性控精液对奶牛体内胚胎质量、发育和移植妊娠率的影响

doi:10.12160/j.issn.1672-5190.2023.01.011

摘要/Abstract

摘要：

[目的] 研究性控精液对奶牛体内胚胎质量、胚胎发育和胚胎移植妊娠率的影响。[方法] 将144头青年奶牛随机分为对照组（63头）和试验组（81头），使用促卵泡激素（FSH，260 mg/头）进行超排处理。对照组和试验组分别使用常规精液和性控精液输精，并对获得的体内性控胚胎进行移植，对胚胎生产、胚胎质量、胚胎发育和胚胎移植妊娠情况进行统计。[结果] 试验组供体获得的平均可用胚胎数（5.67枚）显著（P<0.05）低于对照组（6.92枚）；试验组供体获得的可用胚胎中A级胚胎比例（62.53%）、B级胚胎比例（35.29%）与对照组（A级胚胎比例66.51%、B级胚胎比例30.97%）相比差异均不显著（P>0.05）；试验组供体获得的可用胚胎中桑葚胚比例（84.10%）显著（P<0.05）高于对照组（61.24%），囊胚比例（15.90%）显著（P<0.05）低于对照组（38.76%）；试验组的鲜胚移植妊娠率（52.41%）显著（P<0.05）低于对照组（66.13%）。[结论] 与常规精液相比，使用性控精液生产奶牛体内性控胚胎并移植后，平均可用胚胎数、可用胚胎中囊胚比例和胚胎移植妊娠率降低，可用胚胎质量未明显降低；优化性控精液使用方案和胚胎移植技术能够提高体内性控胚胎生产和胚胎移植效率。

关键词: 奶牛, 性控精液, 超数排卵, 胚胎移植

Abstract:

[Objective] The present study aimed to assess the effects of sex-sorted semen on quality and development of embryo in vivo as well as on the pregnancy rate of embryo transfer in dairy heifers. [Method] A total of 144 dairy heifers served as donors were randomly assigned into a control group （n=63） or an experimental group （n=81）. Follicle stimulating hormone （FSH） was used for superovulation treatment at dosage of 260 mg per heifer. The dairy heifers in the control group and the experimental group were artificially inseminated with normal and sex-sorted semen, respectively. The obtained fresh in vivo-derived sex-sorted embryos were transferred into the recipients. The embryo production, embryo quality, embryo development, and pregnancy rate of embryo transfer were statistically recorded. [Result] The mean number of transferable embryos obtained from the donors in the experimental group （5.67） was significantly （P<0.05） lower than that in the control group （6.92）. The proportions of A-grade embryos （62.53%） and B-grade embryos （35.29%） in the transferable embryos obtained from the donors in the experimental group did not significantly （P>0.05） differ from those in the control group （66.51% of A-grade embryos and 30.97% of B-grade embryos). The donors in the experimental group had a significantly （P<0.05） higher proportion of morula （84.10%） in the transferable embryos in comparison to the control group （61.24%）, while had a significantly （P<0.05） lower proportion of blastocyst （15.90%） compared with the control group （38.76%）. The pregnancy rate of fresh embryo transfer in the experimental group （52.41%） was significantly （P<0.05） lower than that in the control group （66.13%）. [Conclusion] Compared with the use of normal semen and embryo, the use of sex-sorted semen lowered the mean number of transferable embryos and the proportion of blastocyst in the transferable embryos of the donors, and that of sex-sorted embryo decreased the pregnancy rate of the recipients. However, the quality of transferable embryos had no significant reduction. Optimizing the use of sex-sorted semen and the embryo transfer technique can improve the efficiencies of in vivo sex-sorted embryo production and embryo transfer.

Key words: dairy heifers, sex-sorted semen, superovulation, embryo transfer

中图分类号:

S823.913.6

郝海生, 杜卫华, 庞云渭, 邹惠影, 赵学明, 赵善江, 朱化彬. 性控精液对奶牛体内胚胎质量、发育和移植妊娠率的影响[J]. 畜牧与饲料科学, 2023, 44(1): 71-75.

HAO Hai-sheng, DU Wei-hua, PANG Yun-wei, ZOU Hui-ying, ZHAO Xue-ming, ZHAO Shan-jiang, ZHU Hua-bin. Effects of Sex-sorted Semen on In Vivo Embryo Quality，Development and Pregnancy Rate of Embryo Transfer in Dairy Heifers[J]. Animal Husbandry and Feed Science, 2023, 44(1): 71-75.

图/表 4

参考文献 33

[1]	SUH T K, SCHENK J L, SEIDEL G E Jr. High pressure flow cytometric sorting damages sperm[J]. Theriogenology, 2005, 64(5):1035-1048. doi: 10.1016/j.theriogenology.2005.02.002 pmid: 16125550
[2]	SCHENK J L, CRAN D G, EVERETT R W, et al. Pregnancy rates in heifers and cows with cryopreserved sexed sperm: Effects of sperm numbers per inseminate, sorting pressure and sperm storage before sorting[J]. Theriogenology, 2009, 71(5):717-728. doi: 10.1016/j.theriogenology.2008.08.016 pmid: 19150124
[3]	SEIDEL G E Jr. Update on sexed semen technology in cattle[J]. Animal, 2014, 8(Suppl 1):160-164. doi: 10.1017/S1751731114000202
[4]	GARNER D L, SEIDEL G E Jr. History of commercializing sexed semen for cattle[J]. Theriogenology, 2008, 69(7):886-895. doi: 10.1016/j.theriogenology.2008.01.006 pmid: 18343491
[5]	SARTORI R, SOUZA A H, GUENTHER J N, et al. Fertilization rate and embryo quality in superovulated Holstein heifers artificially inseminated with X-sorted sperm[J]. Animal Reproduction, 2004, 1(1):86-90.
[6]	SCHENK J L, SUH T K, SEIDEL G E Jr. Embryo production from superovulated cattle following insemination of sexed sperm[J]. Theriogenology, 2006, 65(2):299-307. pmid: 15964618
[7]	PEIPPO J, VARTIA K, KANANEN-ANTTILA K, et al. Embryo production from superovulated Holstein-Friesian dairy heifers and cows after insemination with frozen-thawed sex-sorted X spermatozoa or unsorted semen[J]. Animal Reproduction Science, 2009, 111(1):80-92. doi: 10.1016/j.anireprosci.2008.02.002 pmid: 18359583
[8]	BARUSELLI P S, MARTINS C M, SALES J N S, et al. Recent advances in bovine superovulation[J]. Acta Scientiae Veterinariae, 2008, 36(2):433-448.
[9]	HAYAKAWA H, HIRAI T, TAKIMOTO A, et al. Superovulation and embryo transfer in Holstein cattle using sexed sperm[J]. Theriogenology, 2009, 71(1):68-73. doi: 10.1016/j.theriogenology.2008.09.016 pmid: 18951623
[10]	AN L, WU Z H, WU Y F, et al. Fertility in single-ovulating and superovulated dairy heifers after insemination with low dose sex-sorted sperm[J]. Reproduction in Domestic Animals, 2010, 45(6):e344-e350. doi: 10.1111/j.1439-0531.2009.01574.x
[11]	LARSON J E, LAMB G C, FUNNELL B J, et al. Embryo production in superovulated Angus cows inseminated four times with sexed-sorted or conventional, frozen-thawed semen[J]. Theriogenology, 2010, 73(5):698-703. doi: 10.1016/j.theriogenology.2009.11.009 pmid: 20053429
[12]	KAIMIO I, MIKKOLA M, LINDEBERG H, et al. Embryo production with sex-sorted semen in superovulated dairy heifers and cows[J]. Theriogenology, 2013, 80(8):950-954. doi: 10.1016/j.theriogenology.2013.07.025 pmid: 23998739
[13]	李强, 牛志红, 刘水涛, 等. 常规精液与性控精液的超数排卵效果比较[J]. 中国奶牛, 2010(7):37-38.
[14]	赵宪林, 昝林森, 田万强, 等. 奶牛性控精液品质检测及效果分析[J]. 畜牧兽医杂志, 2013, 32(2):21-23.
[15]	王建国, 周文忠, 钱松晋, 等. 奶牛X性控冻精应用研究[J]. 广西农业生物科学, 2006, 25(S1):191-192.
[16]	刘霞. 不同种公牛个体分离性控精液受精能力的研究[D]. 北京: 中国农业科学院, 2013.
[17]	毕江华, 冯春涛, 房义, 等. 奶牛性控冻精与常规冻精超排效果的比较[J]. 黑龙江畜牧兽医, 2009(13):60-61.
[18]	巴特尔, 孙伟, 苏和, 等. 用荷斯坦奶牛性控精液生产体内性控胚胎[J]. 动物医学进展, 2012, 33(4):68-71.
[19]	MIKKOLA M, TAPONEN J. Quality and developmental rate of embryos produced with sex-sorted and conventional semen from superovulated dairy cattle[J]. Theriogenology, 2017, 87:135-140. doi: S0093-691X(16)30366-1 pmid: 27662774
[20]	WILSON R D, FRICKE P M, LEIBFRIED-RUTLEDGE M L, et al. In vitro production of bovine embryos using sex-sorted sperm[J]. Theriogenology, 2006, 65(6):1007-1015. doi: 10.1016/j.theriogenology.2005.07.007
[21]	MORTON K M, HERRMANN D, SIEG B, et al. Altered mRNA expression patterns in bovine blastocysts after fertilisation in vitro using flow-cytometrically sex-sorted sperm[J]. Molecular Reproduction and Development, 2007, 74(8):931-940. doi: 10.1002/(ISSN)1098-2795
[22]	BERMEJO-ÁLVAREZ P, RIZOS D, RATH D, et al. Can bovine in vitro-matured oocytes selectively process X- or Y-sorted sperm differentially?[J]. Biology of Reproduction, 2008, 79(4):594-597. doi: 10.1095/biolreprod.108.070169
[23]	PALMA G A, OLIVIER N S, NEUMÜLLER C, et al. Effects of sex-sorted spermatozoa on the efficiency of in vitro fertilization and ultrastructure of in vitro produced bovine blastocysts[J]. Anatomia, Histologia, Embryologia, 2008, 37(1):67-73.
[24]	PEIPPO J, RÄTY M, KORHONEN K, et al. Impact of in vitro fertilization of bovine oocytes with sex-sorted frozen-thawed spermatozoa on developmental kinetics, quality and sex ratio of developing embryos[J]. Zygote, 2010, 18(3):185-194. doi: 10.1017/S0967199409990281
[25]	TRIGAL B, GÓMEZ E, CAAMAÑO J N, et al. In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm[J]. Theriogenology, 2012, 78(7):1465-1475. doi: 10.1016/j.theriogenology.2012.06.018
[26]	王振刚, 孙凤俊, 吕继艳. 性控精液应用研究进展[J]. 中国奶牛, 2009(6):28-30.
[27]	岳奎忠, 罗明久, 武浩楠, 等. 分离后精子输精对超排奶牛卵子受精和胚胎质量的影响[J]. 中国畜牧杂志, 2008, 44(3):10-12.
[28]	SOARES J G, MARTINS C M, CARVALHO N A T, et al. Timing of insemination using sex-sorted sperm in embryo production with Bos indicus and Bos taurus superovulated donors[J]. Animal Reproduction Science, 2011, 127(3/4):148-153. doi: 10.1016/j.anireprosci.2011.08.003
[29]	AN L Y, CHAUBAL S A, LIU Y H, et al. Significant heparin effect on bovine embryo development during sexed in vitro fertilization[J]. The Journal of Reproduction and Development, 2017, 63(2):175-183. doi: 10.1262/jrd.2016-142
[30]	MIKKOLA M, ANDERSSON M, TAPONEN J. Transfer of cattle embryos produced with sex-sorted semen results in impaired pregnancy rate and increased male calf mortality[J]. Theriogenology, 2015, 84(7):1118-1122. doi: 10.1016/j.theriogenology.2015.06.012 pmid: 26174034
[31]	FERRAZ P A, BURNLEY C, KARANJA J, et al. Factors affecting the success of a large embryo transfer program in Holstein cattle in a commercial herd in the southeast region of the United States[J]. Theriogenology, 2016, 86(7):1834-1841. doi: 10.1016/j.theriogenology.2016.05.032 pmid: 27364084
[32]	ERDEM H, KARASAHIN T, ALKAN H, et al. Effect of embryo quality and developmental stages on pregnancy rate during fresh embryo transfer in beef heifers[J]. Tropical Animal Health and Production, 2020, 52(5):2541-2547. doi: 10.1007/s11250-020-02287-6 pmid: 32445155
[33]	ZNANIECKI R, JASKOWSKI J, ZNANIECKA E. Wplyw niektorych czynnikow na wyniki zacielen u jalowic biorczyn swiezych zarodkow[J]. Medycyna Weterynaryjna, 1998, 54:550-553.