荷斯坦奶牛与和牛活体采卵-体外受精（OPU/IVF）体系的建立及应用

doi:10.12160/j.issn.1672-5190.2023.03.001

畜牧与饲料科学 ›› 2023, Vol. 44 ›› Issue (3): 1-6.doi: 10.12160/j.issn.1672-5190.2023.03.001

• 科技创新支撑畜牧业高质量发展专栏——新理论新技术在畜牧业中的应用专题 • 下一篇

荷斯坦奶牛与和牛活体采卵-体外受精（OPU/IVF）体系的建立及应用

周璇¹,苏日娜²,李秀男¹,白苏友拉图²,乌达巴拉¹,杨燕燕¹,乌日罕¹,何亭漪¹,乌吉斯古楞²,王标¹,何牧仁²

1.内蒙古自治区农牧业科学院/肉羊遗传资源评价与繁育技术国家地方联合工程研究中心，内蒙古呼和浩特 010031
2.草原和牛投资有限公司，内蒙古呼和浩特 010000

收稿日期:2023-04-10 出版日期:2023-05-30 发布日期:2023-07-12
通讯作者: 王标（1985—），男，助理研究员，博士，主要从事家畜繁殖育种研究工作。何牧仁（1966—），男，研究员，博士，主要从事家畜繁殖育种工作。
作者简介:周璇（1986—），男，助理研究员，硕士，主要从事家畜繁殖技术研究工作。|苏日娜（1995—），女，硕士，主要从事家畜繁殖技术研究工作。
基金资助:
内蒙古自治区科技计划项目(2021GG0210);呼和浩特市重大科技专项(2021-农-重-3)

Establishment and Application of Ovum Pick-up and In Vitro Fertilization（OPU/IVF）in Holstein Dairy Cow and Wagyu Cattle

ZHOU Xuan¹,Surina ²,LI Xiunan¹,BAI Suyoulatu²,Wudabala ¹,YANG Yanyan¹,Wurihan ¹,HE Tingyi¹,Wujisiguleng ²,WANG Biao¹,HE Muren²

1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences/National Local Joint Engineering Research Center of Sheep Genetic Resources Evaluation and Breeding Technology，Hohhot 010031，China
2. Grassland and Cattle Investment Co.，Ltd.，Hohhot 010000，China

Received:2023-04-10 Online:2023-05-30 Published:2023-07-12

摘要/Abstract

摘要：

[目的]研究牛活体采卵-体外受精（ovum pick-up and in vitro fertilization，OPU/IVF）体系，建立高效的牛体外胚胎生产系统。[方法]以从屠宰场采集的健康牛新鲜卵巢为试验材料，进行卵母细胞体外成熟、体外受精、体外胚胎培养相关条件的摸索，重点考查体外胚胎培养液中添加瘦素（leptin）对囊胚率的影响。选取13~15月龄健康荷斯坦奶牛及和牛各10头作为供体，进行活体采卵、卵母细胞体外成熟、体外胚胎生产，记录可用卵数及可用囊胚数，统计卵裂率、囊胚率;2个品种牛的体外冷冻胚胎解冻后，以荷斯坦奶牛为受体进行胚胎移植，移植后45 d统计妊娠率。[结果]添加30 U/mL的leptin可以显著（P<0.05）提高屠宰场来源牛体外胚胎的囊胚率。随机选择供体牛活体采卵，平均每头荷斯坦奶牛获得可用卵7.5枚，平均每头和牛获得可用卵8.1枚;荷斯坦奶牛及和牛的卵裂率分别为84.00%、82.71%，二者差异不显著（P>0.05）。体外培养条件下，平均每头荷斯坦奶牛获得可用囊胚4.3枚，平均每头和牛获得可用囊胚3.7枚;荷斯坦奶牛的囊胚率（57.33%）显著（P<0.05）高于和牛（45.68%）。体外胚胎解冻后移植，荷斯坦奶牛胚胎组的妊娠率为54.17%，和牛胚胎组的妊娠率为55.79%，二者差异不显著（P>0.05）。[结论]该研究建立的OPU/IVF体系可以有效提高优质母牛的利用效率，降低生产成本，为牛体外胚胎生产系统的优化提供了参考。

关键词: 荷斯坦奶牛, 和牛, 活体采卵-体外受精, 体外胚胎生产

Abstract:

[Objective] This study was conducted to explore the ovum pick-up and in vitro fertilization （OPU/IVF） technology in cattle, and to establish a high-efficient in vitro embryo production system for cattle. [Method] The fresh ovaries of healthy cattle collected from slaughterhouses were used as experimental materials to perform oocyte maturation as well as fertilization and embryo culture under in vitro conditions, with focus on the effect of leptin supplementation in culture medium on blastocyst rate. Healthy Holstein dairy cows （n=10） and Wagyu cattle（n=10） aged 13 to 15 months were selected as donors, and ovum pick-up as well as oocyte maturation and embryo production under in vitro conditions were carried out. For both breeds, the number of available oocytes and blastocysts was recorded, and the cleavage rates and blastocyst rates were calculated. After thawing of the prepared frozen embryos of the donors, Holstein dairy cows were used as recipients for embryo transfering. The pregnancy rates of the recipients receiving the in vitro produced embryos of the Healthy Holstein dairy cows and Wagyu cattle were measured on day 45 after embryo transfering. [Result] Addition of 30 U/mL leptin in the culture medium significantly （P<0.05） increased the blastocyst rate of the in vitro cultured cattle embryos of slaughterhouse origin. For the donors, 7.5 and 8.1 usable oocytes were averagely obtained per Holstein dairy cow and per Wagyu cattle, respectively. The cleavage rates of Holstein dairy cow and Wagyu cattle were 84.00% and 82.71%, respectively, and there was no significant （P>0.05） difference between them. Under the in vitro conditions, 4.3 and 3.7 available blastocysts were averagely obtained per Holstein dairy cow and per Wagyu cattle, respectively, and the blastocyst rate of Holstein dairy cow （57.33%） was significantly （P<0.05） higher than that of Wagyu cattle （45.68%）. The pregnancy rates of Holstein dairy cow embryo group and Wagyu cattle embryo group were 54.17% and 55.79%, respectively, and no significant （P>0.05） difference was observed. [Conclusion] The established OPU/IVF system effectively improves the utilization efficiency of high-quality female cattle, reduces its production costs, and provides references for the optimization of in vitro embryo production system for cattle.

Key words: Holstein dairy cow, Wagyu cattle, OPU/IVF, in vitro embryo production

中图分类号:

S823.913.6

周璇, 苏日娜, 李秀男, 白苏友拉图, 乌达巴拉, 杨燕燕, 乌日罕, 何亭漪, 乌吉斯古楞, 王标, 何牧仁. 荷斯坦奶牛与和牛活体采卵-体外受精（OPU/IVF）体系的建立及应用[J]. 畜牧与饲料科学, 2023, 44(3): 1-6.

ZHOU Xuan, Surina , LI Xiunan, BAI Suyoulatu, Wudabala , YANG Yanyan, Wurihan , HE Tingyi, Wujisiguleng , WANG Biao, HE Muren. Establishment and Application of Ovum Pick-up and In Vitro Fertilization（OPU/IVF）in Holstein Dairy Cow and Wagyu Cattle[J]. Animal Husbandry and Feed Science, 2023, 44(3): 1-6.

图/表 3

参考文献 28

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荷斯坦奶牛与和牛活体采卵-体外受精（OPU/IVF）体系的建立及应用

Establishment and Application of Ovum Pick-up and In Vitro Fertilization（OPU/IVF）in Holstein Dairy Cow and Wagyu Cattle

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