库车市某规模化猪场猪源产酸克雷伯菌的分离鉴定及其部分生物学特性研究

doi:10.12160/j.issn.1672-5190.2025.03.016

畜牧与饲料科学 ›› 2025, Vol. 46 ›› Issue (3): 108-113.doi: 10.12160/j.issn.1672-5190.2025.03.016

库车市某规模化猪场猪源产酸克雷伯菌的分离鉴定及其部分生物学特性研究

郭文泽, 杨彬, 马雯萱, 李静, 王泽坤, 于月通, 马志远, 齐萌

塔里木大学动物科学与技术学院,新疆阿拉尔 843300

收稿日期:2025-03-16 出版日期:2025-05-30 发布日期:2025-09-02
通讯作者: 齐萌（1985—）,男,教授,博士,博士生导师,主要研究方向为人兽共患病病原生物学。
作者简介:郭文泽（1999—）,男,硕士研究生,主要研究方向为动物群发病防治。
基金资助:
新疆生产建设兵团英才支持计划青年项目

Isolation, Identification and Biological Characteristics of Klebsiella oxytoca from Pigs in a Large-scale Pig Farm in Kuqa City

GUO Wenze, YANG Bin, MA Wenxuan, LI Jing, WANG Zekun, YU Yuetong, MA Zhiyuan, QI Meng

College of Animal Science and Technology, Tarim University, Alaer 843300, China

Received:2025-03-16 Online:2025-05-30 Published:2025-09-02

摘要/Abstract

摘要： [目的]调查库车市某规模化猪场猪鼻拭子中产酸克雷伯菌的感染情况,研究其生物学特性。[方法]采集该猪场80份有呼吸道感染症状的猪鼻拭子样本,经细菌分离培养和16S rDNA测序比对分析鉴定细菌种类;通过检测分离菌株对抗菌药物的药物敏感性和耐药基因携带情况,了解其耐药性;通过动物感染试验评定其致病性。[结果]对80份猪鼻拭子样本进行细菌分离培养,经形态学鉴定,初步分离出18株疑似克雷伯属细菌;经16S rDNA测序比对分析,18株菌株中鉴定出3株为产酸克雷伯菌。选择10类14种抗菌药物进行药物敏感试验,3株产酸克雷伯菌均为多重耐药菌株;对分离株进行20种耐药基因检测,3株产酸克雷伯菌共检测出bla_TEM、tetA、sul1、sul2、tetB、aac（3′）-IIa、aadA和qnrB 8种耐药基因;动物致病性试验发现,3株产酸克雷伯菌分离株均可引起小鼠死亡,具有较强的致病性。[结论]该规模化猪场分离的产酸克雷伯菌呈现多重耐药,携带多种耐药基因,对小鼠具有较强的致病性。本研究结果为该规模化猪场产酸克雷伯菌病的防治提供了基础数据。

关键词: 产酸克雷伯菌, 分离鉴定, 耐药性, 致病性, 猪

Abstract: [Objective] To investigate the infection of Klebsiella oxytoca in the swabs of pigs from a large-scale pig farm in Kuqa City, and to study its biological characteristics. [Methods] 80 nasal swab samples with respiratory tract infection symptoms were collected from the pig farm, and the bacterial species were identified by bacterial isolation and 16S rDNA sequencing. The drug resistance of the isolated strains was understood by detecting the drug sensitivity of the isolated strains to antibiotics and the carrying of drug resistance genes. The pathogenicity was evaluated by animal infection test. [Results] Bacterial isolation and culture were performed on 80 pig nasal swab samples. After morphological identification, 18 strains of suspected Klebsiella bacteria were initially isolated. After 16S rDNA sequencing and comparison analysis, three of the 18 strains were identified as K. oxytoca. 14 antimicrobial drugs from 10 classes were selected for drug sensitivity test, and threestrains of K. oxytoca were multidrug-resistant strains. The isolates were tested for 20 drug-resistant genes, and a total of 8 resistance genes were detected in the three strains of K. oxytoca, including bla_TEM, tetA, sul1, sul2, tetB, aac （3′）-IIa, aadA and qnrB. It was found that all the three isolates of acid-producing K. oxytoca could cause the death of mice and had strong pathogenicity. [Conclusion] The acid-producing K. oxytoca isolated from the large-scale pig farm showed multidrug resistance, carried multidrug-resistant genes, and had strong pathogenicity to mice. The results of this study provided basic data for the prevention and control of acid-producing K. oxytoca in this large-scale pig farm in Kuqa.

Key words: Klebsiella oxytoca, isolation and identification, drug resistance, pathogenicity, pig

中图分类号:

S858.282.61

郭文泽, 杨彬, 马雯萱, 李静, 王泽坤, 于月通, 马志远, 齐萌. 库车市某规模化猪场猪源产酸克雷伯菌的分离鉴定及其部分生物学特性研究[J]. 畜牧与饲料科学, 2025, 46(3): 108-113.

GUO Wenze, YANG Bin, MA Wenxuan, LI Jing, WANG Zekun, YU Yuetong, MA Zhiyuan, QI Meng. Isolation, Identification and Biological Characteristics of Klebsiella oxytoca from Pigs in a Large-scale Pig Farm in Kuqa City[J]. Animal Husbandry and Feed Science, 2025, 46(3): 108-113.

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库车市某规模化猪场猪源产酸克雷伯菌的分离鉴定及其部分生物学特性研究

Isolation, Identification and Biological Characteristics of Klebsiella oxytoca from Pigs in a Large-scale Pig Farm in Kuqa City

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