Animal Husbandry and Feed Science ›› 2010, Vol. 31 ›› Issue (3): 13-13.doi: 10.12160/j.issn.1672-5190.2010.03.006

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Cloning and Sequencing of Neuropeptide Y Gene of Dairy Cows

WU Jia-di,FEI Dong-liang,ZHANG Cai (1.College of Animal Husbandry and Veterinary Medicine,Liaoning Medical University,Jinzhou 121001,China;2.College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China)   

  • Online:2010-03-20 Published:2010-03-20

Abstract: According to the sequence of neuropeptide Y (NPY) gene published in GenBank,a pair of primers was designed.With cDNA extracted from calf hypothalamus as a template,NPY gene was amplified by polymerase chain reaction (PCR).The PCR products were cloned,sequenced and analyzed.The results showed that the designed primers could be used to amplify NPY gene of cow,and the amplified NPY gene had 100% nucleic acid homology to that published in GenBank.

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