Animal Husbandry and Feed Science ›› 2011, Vol. 32 ›› Issue (7): 39-39.doi: 10.12160/j.issn.1672-5190.2011.07.021

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Determination of Tryptophan in Forage Feather Peptide Powder

LIN Xiang-xin,YAO Qing-hua,SONG Yong-kang(1.Central Laboratory,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China;2.Fujian Key Laboratory of Agricultural Testing by Precision Instruments,Fuzhou 350003,China)   

  • Online:2011-07-20 Published:2011-07-20

Abstract: The effects of various alkali-hydro agents and alkali-hydrolyzable conditions on the determination of tryptophan in the forage feather peptide powder were compared. The results showed that the effect of hydrolyzing with 4 mol/L LiOH at(110±1) ℃ for 20 h was the best,the effect of hydrolyzing with 6 mol/L NaOH and 3% SnCl2 at(110±1) ℃ for 20 h was the next. The effects of the dose of alkali-hydro agent,alkali-hydrolyzable temperature,the concentration of atmosphere and ammonia in the alkali-hydrolyzable tube on the determined value of tryptophan in the determination were further discussed.

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