Abstract: This study was conducted to evaluate the influence of dietary supplementation of Saccharomyces cerevisiae culture on fattening Wagyu. A total of 374 healthy fattening Wagyu with similar weight were selected and assigned into the following three groups: group A and group B were set as experimental groups whose basal diet was supplemented with different levels of Saccharomyces cerevisiae culture, and group C served as control group and received no dietary supplementation;all of the fattening Wagyu fed three times daily (morning, noon and night), and the formal experiment lasted for 45 days;at the end of the experiment, the Wagyu in both experimental groups and control group were weighted, and the daily gain of the Wagyu in different groups during the experiment were statistically assessed;the feed digestibility of the Wagyu in different groups were evaluated by using manure screener. The results showed that the daily gain of group A and group B were significantly higher than group C (P<0.05), and the highest value was observed in group B;at the end of the experiment, the percent of bottom screen in group A and group B were elevated compared to that at the start of the experiment, and the content of yellow corn chips in bottom screen in group A and group B were fewer than group C. In conclusion, dietary supplementation of Saccharomyces cerevisiae culture in basal diet can improve the daily gain and feed digestibility of fattening Wagyu.