Abstract: [Objective]To establish a determination method of Zn in fish by digestion tank digestion and flame atomic absorption spectrometry. [Method]The fish was dissolved by digestion tank digestion, and then Zn in fish was determined by flame atomic absorption spectrometry. [Result]This method had good linear relation when the concentration range of Zn was 0-2.0 mg/L, and the correlation coefficient was 0.999 7. The detection limit of Zn was 0.002mg/mL, with the relative standard deviation of 3.12%. The recovery of Zn was 95.0%-102.5%. [ Conclusion ]The method is simole, raoid and accurate, so it is suitable for the determination of Zn in fish.