Abstract: Higher vocational colleges must start from students′ learning interest and actual situation, and they could train out highly-qualified and fully-developed talents only if the reform on pharmacological animal practical teaching in higher vocational colleges is strengthened further. The teaching content reform, pushing the co -operation between colleges and enterprises, improving conditions of experiment and training and strengthening practical spot teaching in the reform on pharmacological animal practical teaching in higher vocational colleges were discussed in order to provide reference for higher vocational colleges to implement practical teaching reform.