Abstract: The spleen asthenia syndrome model in mice was prepared by intraperitoneal injection of reserpine. The mice with spleen asthenia syndrome were given donkey milk by intragastric administration for fifteen consecutive days. The model control group and blank control group were also set in this study. After the last administration, the number of blood cells and the organ index of spleen and thymus were determined. The results showed that the number of blood cells and organ index of spleen and thymus of the model control group were significantly less and lower than those of the blank control group（P〈0.05）; compared to the model control group, the donkey milk administration group had significantly more blood cells and significantly higher organ index of spleen and thymus（P〈0.05）. The combined data suggest that donkey milk has a protective effect on the tissue cells of mice with spleen asthenia syndrome.