Animal Husbandry and Feed Science ›› 2023, Vol. 44 ›› Issue (3): 103-108.doi: 10.12160/j.issn.1672-5190.2023.03.015

• Food Science • Previous Articles     Next Articles

Isolation and Identification of Yeasts from Sour Whey in Xilin Gol League, Inner Mongolia

ZHENG Wei1,KANG Lianhe1,LIU Pengbin2,Terigele 1,LI Zijian1,LI Xingyun1,WANG Limei1,ZHANG Yuanyuan1,LIU Xiuli1,SHI Pei1,QIAO Jianmin1   

  1. 1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China
    2. Alagxa League Comprehensive Inspection and Testing Center for Agricultural and Livestock Products Quality,Alagxa Left Banner 750300,China
  • Received:2023-05-09 Online:2023-05-30 Published:2023-07-12

Abstract:

[Objective] This study aimed to explore the biodiversity of yeasts in sour whey, a by-product of traditional cheese processing in the pastoral area of Xilin Gol League, Inner Mongolia. [Method] The yeasts were isolated and purified from two sour whey samples collected in Xilin Gol League, Inner Mongolia, using traditional isolation and culture technology of microorganisms. The species of the isolated yeast strains was determined by ITS gene sequence analysis, and a phylogenetic tree was constructed. [Result] A total of 18 yeast strains were obtained from the two sour whey samples, belonging to 4 different species, Saccharomyces cerevisiaen=2), Pichia fermentansn=2), Pichia kudriavzeviin=2) and Kluyveromyces marxianusn=12). Among them, Kluyveromyces marxianus was the dominant flora in the sour whey samples. [Conclusion] Our results reveal the biodiversity of yeasts in sour whey collected in Xilin Gol League, Inner Mongolia, and provide references for the comprehensive utilization and industrial production of sour whey.

Key words: sour whey, yeast, biodiversity, isolation and identification

CLC Number: