Abstract: The Peelplate 3M plate detecting method and national standard method were simultaneously used to determine the colony count in raw milk samples collected from Hulunbuir,Tongliao and Hohhot,respectively,and the detecting results of these two methods were statistically compared with variance analysis.The results of Peelplate 3M plate detecting method with different sample incubation time(24 h and 48 h)were also statistically compared with variance analysis.There were no significant differences in detecting results of colony count in raw milk samples from the three regions between Peelplate 3M plate detecting method and national standard method(P>0.05);when using the Peelplate 3M plate detecting method,there was no significant difference in colony count determination results between the sample incubation duration of 24 h and 48 h(P>0.05).Our results demonstrate that the Peelplate 3M plate detecting method can serve as an alternative to the national standard method in raw milk colony count determination.In addition,the 24 h of sample incubation can produce reliable results in Peelplate 3M plate detecting method.