Abstract: The aim of the present study was to assess the effect of different diluents on semen quality of Lezhi black goat. Three kinds of diluents including citric acid - Tris （No.1）, phosphate （No.2） and OviXcell （No.3） were prepared; fresh semen samples were collected from eight Lezhi black goats and were subsequently cryopreserved with the three kinds of diluents, respectively; the sperm motility, sperm abnormality rate and sperm acrosome intact rate of the semen samples cryopreserved with different diluents at the time points of post collection, post balance and post thawing were determined and compared, respectively. The results showed that the highest sperm motility and sperm acrosome intact rate as well as the lowest sperm abnormality rate were observed in the semen samples cryopreserved with OviXcell （No.3）. It is indicated that the diluent of OviXcell has superior performance in in vitro cryopreservation of Lezhi black goat semen.