Abstract: In order to investigation the effect of different concentrations of tetrazole solution and different dyeing temperature on the determination of seed viability of Helianthus tuberosus, the optimum conditions of seed viability of Helianthus tuberosus determination were screened according to the principle of tetrazole dyeing method （TTC）. The results showed that the best dyeing effect was obtained under the following conditions： after completely soaked in the distilled water at 25 %, the seeds of Helianthus tuberosus were dyed using TTC with concentration of 0.5% for 7 hours under dark condition of 35 %. The seeds viability can be well determined and their potential germination ability can be well estimated using the above method. This study provides references for the rapid determination of seed viability of Helianthus tuberosus.