Abstract: [Objective]To assess the effects of biological fermented feed on productive performance and egg quality of laying hens.[Methods]A total of 8 000 healthy 367-day-old Highland Brown laying hens were randomly assigned into two groups： a control group （n=4 000） fed with basal diet, and an experimental group （n=4 000） fed with a fixed diet supplemented with 4% fermented palm kernel cake in basal diet. The experimental period lasted for 45 days. At the end of the experiment, the productive performance and egg quality of the laying hens in experimental group and control group were determined and statistically evaluated. [Results] At the 45th day of the experiment, the average daily egg weight and the ratio of feed to egg of experimental group were increased by 6.62% （P〈0.05） and reduced by 4.56% （P〈0.05） compared to control group, respectively; the albumen height and Haugh unit of laying hens in the experimental group were both significantly elevated compared to that in the control group, which were increased by 9.56% （P〈0.05） and 6.31% （P〈0.05）, respectively; the experimental group had extremely significantly higher yolk color degree compared to the control group （P〈0.01）.[Conclusion]The supplementation of fermented palm kernel cake in basal diet can improve the productive performance and egg quality of laying hens.