Abstract: In this study, we tested whether Rhodiola extracts had any effects on antibody responses to infectious bursal disease virus (IBDV)and sheep red blood cells (SRBC)in broiler chickens reared high-altitude hypoxia condition. A total of 100 1-day-old healthy AA broilers were randomly divided into 5 treatments. Four treatments received dietary supplementation of 0.10%, 0.15%, 0.20%and 0.40%dry powder of Rhodiola aqueous extracts in basal diet, respectively, and the other group serving as control fed basal diet without supplementation. Half of the broilers in each treatment were injected intraperitoneally 1 mL of a 10% suspension of SRBC at 13 d of age, and the others were orally vaccinated at 20 d of age with the Bursine-2 live virus. The results showed that compared to the control group, supplementation of 0.20%and 0.40% Rhodiola extracts significantly increased the IBDV antibody titer at 2 d and 19 d after vaccination (P<0.05), and increased the SRBC antibody titer at 5 d, 8 d and 12 d after inoculation (P<0.05). In conclusion, dietary supplementation with Rhodiola extracts could increase the specific antibody level in broiler chickens reared at high-altitude hypoxia condition. Therefore, Rhodiola extracts may be act as an immune regulator additive for broilers reared high-altitude hypoxia condition.