GCA对小鼠腹腔巨噬细胞抗氧化作用的影响

doi:10.12160/j.issn.1672-5190.2020.04.015

Animal Husbandry and Feed Science ›› 2020, Vol. 41 ›› Issue (4): 77-80.doi: 10.12160/j.issn.1672-5190.2020.04.015

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Effect of Glycocholic Acid（GCA）on Anti-oxidation Capacity of Mouse PeritonealMacrophages

Wuyunfu¹, SHI Lin-lin^2,3, LI Pei-feng^2,3, GUAN Hong^2,3, YAO Qing-zhi⁴, HE Xiu-ling^2,3

1. Electronic Support Center,Organization Establishment Committee Office of Alagxa League of Inner Mongolia,Alagxa Left Banner 750306,China;
2. College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;
3. Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease,Ministry of Agriculture and Rural Affairs,Hohhot 010018,China;
4. College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China

Received:2020-03-24 Online:2020-07-30 Published:2020-08-19

Abstract

Abstract: This study aimed to assess the antioxidative activity of glycocholic acid （GCA）, one of the extracts from bile of cattle and sheep, on mouse peritoneal macrophages. A total of 5 experimental groups with mass concentrations of 1×10^-3, 1×10^-4, 1×10^-5, 1×10^-6 and 1×10^-7 μg/mL of GCA were included. The mouse peritoneal macrophages were incubated with different concentrations of GCA for 24 hours, and the activities of glutathione peroxidase （GSH-Px）and superoxide dismutase （SOD）, as well as the level of malondialdehyde （MDA）, were determined according to the methods provided in the kit instructions. The results demonstrated that GCA reduced the level of MDA and elevated the activities of GSH-Px and SOD in mouse peritoneal macrophages, indicating its effective role in regulating the level of antioxidative stress in mouse peritoneal macrophages. In conclusion, GCA has the capacity to improve the antioxidative stress response.

Key words: glycocholic acid, mouse peritoneal macrophages, glutathione peroxidase, superoxide dismutase, malondialdehyde

CLC Number:

R285
S853.7

Wuyunfu, SHI Lin-lin, LI Pei-feng, GUAN Hong, YAO Qing-zhi, HE Xiu-ling. Effect of Glycocholic Acid（GCA）on Anti-oxidation Capacity of Mouse PeritonealMacrophages[J]. Animal Husbandry and Feed Science, 2020, 41(4): 77-80.

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Effect of Glycocholic Acid（GCA）on Anti-oxidation Capacity of Mouse PeritonealMacrophages

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