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Table of Content
30 July 2020, Volume 41 Issue 4
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  • Rapid Determination of Calcium and Phosphorus Contents in Shrimp Premix Feed by ED -XRF Method
    LIU Zhao-gui, ZHANG Hui, ZHAO Peng, WU Min, LI Qiang
    2020, 41(4):  1-7.  doi:10.12160/j.issn.1672-5190.2020.04.001
    Abstract ( 425 )   PDF (649KB) ( 88 )   Save
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    The contents of calcium (Ca) and phosphorus (P) in premix feed directly affected the growth, development and health status of shrimp. Traditional chemical analysis methods required complicated pre-treatment process for shrimp premix feed and always took several hours to obtain test results. In this study, a simple and fast analysis method, energy dispersive X-ray fluorescence spectrometry (ED-XRF), was used to assess the contents of Ca and P in shrimp premix feed, and the test results of the samples were obtained within a few minutes. The pre-treatment process of ED-XRF method only included comminution and squash. The limit of detection of ED-XRF method for Ca and P were 43 mg/kg and 163 mg/kg, respectively, and the limit of quantification of that for Ca and P were 142 mg/kg and 544 mg/kg, respectively. Six consecutive determinations of shrimp premix feed sample 3# were conducted by ED-XRF method. The range of the continuous measurement data of Ca and P were 0.159% and 0.013%, respectively, which were smaller than the repeatability critical difference of CrR95 (6) for the contents detection of Ca (0.239%) and P (0.031%) in the recommended national standard, indicating that the ED-XRF method met the requirements of the recommended national standard for the determination of Ca and P contents in feed. By analyzing the contents of Ca and P of ten shrimp premix feed samples which were measured by national standard methods, we found that the relative differences in measured value of Ca and P contents between ED-XRF method and recommended national standard method did not exceeded 4.55%. To sum up, the ED-XRF method had high precision and accuracy, and it could be used in monitoring the addition of Ca and P for shrimp premix feed production enterprises.
    Investigation, Detection and Analysis of Pollution Status of Fumonisins (B1+B2), Ochratoxin A, T-2 Toxin and Aflatoxin B1 in 17 Plant Feed Raw Materials
    HUANG Zhi-wei, FENG Jin, LIU Li-xiao, LI Hong-bo
    2020, 41(4):  8-12.  doi:10.12160/j.issn.1672-5190.2020.04.002
    Abstract ( 335 )   PDF (444KB) ( 128 )   Save
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    This study aimed to understand and master the pollution status of fumonisins (B1+B2), ochratoxin A, T-2 toxin and aflatoxin B1 in plant feed raw materials, guide and help feed production enterprises as well as livestock and poultry breeding enterprises to carry out the prevention and control of mycotoxins pollution, avoid the harms brought by mycotoxins to feed products as well as livestock and poultry feeding, and reduce the economic losses. In the year of 2019, a total of 62 samples from 17 kinds of plant feed raw materials were collected and tested by LC-MS/MS and IAC-HPLC according to the national standard of ′Feed Hygiene Standard′ (GB 13078-2017). The results showed that the pollution status of fumonisins (B1+B2), ochratoxin A and aflatoxin B1 in the 17 kinds of plant feed raw materials obviously varied, the average detection rates of fumonisins (B1+B2), ochratoxin A and aflatoxin B1 were 37.09%, 8.06% and 29.03%, respectively, and their maximum detection values were 15.96 mg/kg, 26.60 μg/kg and 351.00 μg/kg, respectively. According to the test results, the pollution status caused by these 4 kinds of mycotoxins in the 17 kinds of plant feed raw materials was relatively heavy, and the overall pollution rate was 48.40%. The content of aflatoxin B1 in corn bran, spouting corn bran and peanut meal exceeded the limits required by the national standard, but the pollution rate was not proportional to the exceeding standard rate. It is indicated that the mycotoxins pollution in plant feed raw materials is widespread, which results in serious impacts to feed products and feeding safety. In view of this problem, specific suggestions on controlling the quality of plant feed raw materials were put forward, in hoping to provide reference for controlling the contents of mycotoxins in feed raw materials.
    Effect of Adding Lactic Acid in Drinking Water on Growth Performance and Disease Resistance of Sanhuang Chickens
    WANG Shu-yan, SHEN Qian-cheng, LING Ding, LIANG Zhu-min
    2020, 41(4):  13-16.  doi:10.12160/j.issn.1672-5190.2020.04.003
    Abstract ( 664 )   PDF (477KB) ( 90 )   Save
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    [Objective] To assess the effect of adding lactic acid in drinking water on the growth performance and disease resistance of Sanhuang chickens. [Method] A total of 5 000 one-day-old Sanhuang chickens were selected and randomly divided into 5 groups with 1 000 chickens in each group. Lactic acid was added in drinking water of the four experimental groups of A, B, C and D to adjust the pH value to 2.0, 3.0, 4.0 and 5.0, respectively. The chickens in the experimental groups were given acidified water for 4 hours per day, and the control group received normal drinking water. The body weight and feed intake of the chickens were measured, the number of dead and diseased chickens per day was recorded, and the indicators such as average daily gain, feed conversion rate and mortality rate were calculated. [Result] (1) From 1 to 35 days of age, the group C had the most obviously increase in average daily gain which was elevated by 3.33%; after 36 days of age, the group B had the similar level in average daily gain with the control group, while the other experimental groups were reduced. (2)The feed intake and feed conversion rate of the groups B and C had significant (P<0.05) differences compared with the control group, and no significant (P>0.05) differences in those between the groups A and D were observed. (3)The mortality rate of the groups A, B, C and D was reduced by 3.7%, 4.2%, 4.7% and 2.1% compared with the control group, respectively. [Conclusion] In the feeding experiment, the feed conversion rate of the Sanhuang chickens is effectively improved when the pH value of drinking water is adjusted to 4.0 by adding lactic acid. Furthermore, the average daily gain of Sanhuang chickens aged 1 to 35 days is obviously increased, and the mortality rate is obviously reduced. It is indicated that adding appropriate level of lactic acid in drinking water has good application prospects in terms of improving growth performance and disease resistance in broilers.
    Differentiation and Formation of Adipocytes and Regulative Role of Natural Organic Compounds
    YIN Wei-wen, ZHANG Zhi-chao, WANG Xiao-qi, DUAN Zi-yuan
    2020, 41(4):  17-27.  doi:10.12160/j.issn.1672-5190.2020.04.004
    Abstract ( 578 )   PDF (964KB) ( 98 )   Save
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    The elucidation of fat synthesis mechanisms and related regulative process is meaningful for preventing and curing a variety of obesity-associated diseases, ameliorating meat flavor and quality, and improving livestock production efficiency. Adipocytes originate from pluripotent mesenchymal stem cells (MSCs) in bone marrow. After receiving extracellular stimulating factors, they quickly induce the expression of early fat regulatory factors, C/EBPβ and C/EBPδ, and transmit information to PPARγ, C/EBPα and other transcription factors that promote cells differentiation and lipid droplets formation. There are varieties of transcription factors and cyclins involved in the process of differentiation of pre-adipocytes to adipocytes and adipogenesis. Studies have shown that some natural organic compounds derived from plants, such as polyphenols, alkaloids, terpenes and quinones, play important regulative roles in differentiation of adipocytes and inhibition of adipogenesis. More investigations on natural products must be done before developing medicines treatment for obesity-associated diseases through suppression of lipid droplets accumulation in cells.
    Analysis of Molecular Markers Genetically Associated with the Excellent Flight Performance of Pigeon Based on Pectoral Muscles Transcriptome
    LIU Na-na, BAI Xue, SUN Yan-yong, Terigele, FU Shao-yin, DUAN Shi, LIU Yong-bin, ZHANG Wen-guang
    2020, 41(4):  28-31.  doi:10.12160/j.issn.1672-5190.2020.04.005
    Abstract ( 300 )   PDF (582KB) ( 108 )   Save
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    [Objective] To compare and analyze the transcriptome of pectoral muscle tissues between racing pigeons and ornamental pigeons, and to explore the key genes related to flight performance in pigeon pectoral muscles. [Method] Through differential analysis, the differentially expressed genes that were significantly up-and down-regulated in pectoral muscles between racing pigeons and ornamental pigeons were obtained and enriched. [Result] A total of 18.3 G effective data were obtained by using data quality control. A total of 477 differentially expressed genes, including 194 up-regulated genes and 130 down-regulated genes were screened out. Three candidate genes related to pigeon flight performance were screened out by using KEGG pathway significance enrichment analysis : MUSTN1, PDK4 and MSTN. [Conclusion] By comparing the transcriptome data of the pectoral muscles between racing pigeons and ornamental pigeons, the key genes associated with flight performance of pigeon were obtained. The information provided by this study enriched the genomic information of pigeons and laid the foundation for further elaborating the molecular mechanism of pigeon pectoral muscles.
    Effect of FecB Gene on Lambing Performance of Germany Mutton Merino Sheep×Small-tailed Han Sheep×Fine-wool Sheep Ternary Hybrid Sheep
    WANG Wei-xia, TONG Gui-zhi, LIU Xue-feng, Hai Long, WANG Jia-hou, GUO Li-hong, ZHANG Li-chun, LI Xin-tao
    2020, 41(4):  32-35.  doi:10.12160/j.issn.1672-5190.2020.04.006
    Abstract ( 368 )   PDF (639KB) ( 67 )   Save
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    The objectives of this study were to verify the pleomorphic distribution of FecB gene in the ternary hybrid sheep of Germany Mutton Merino sheep×small-tailed Han sheep×fine-wool sheep, and to assess its correlation with lambing performance of the ternary hybrid sheep. German Mutton Merino sheep and the F2 hybrid sheep produced with small-tailed Han rams and northeast fine-wool ewes were used as the male parent and female parent, respectively, to produce the ternary hybrid sheep. The KASP method was used to analyze the genotype of FecB, and its gene frequency and genotype frequency were calculated. Furthermore, the correlation of gene frequency of FecB with the lambing performance of the ternary hybrid sheep flock was assessed. FecB gene was absent in German Mutton Merino sheep. The B allele frequency of the ternary hybrid sheep was increased significantly and was in a Hardy-weinberg imbalance state, indicating that the artificial selection had a great impact on FecB gene enrichment in hybrid sheep. The rank-sum test analysis on litter size of the individuals of F2 hybrid ewes and ternary hybrid sheep with different genotypes demonstrated the P value were all less than 0.01, and for the two sheep flocks, the average lambing rate of the individuals with ++ genotype was both lower than those with B+ and BB genotypes (P<0.05). It is indicated that the B allele play an important role in improving lambing performance of the ternary hybrid sheep. This study provides a reference for ameliorating the lambing performance of the ternary hybrid sheep using FecB gene.
    Analysis on Vegetation Restoration of Degraded Alpine Meadow during Continuous Enclosure Period
    LUO Yong, XUE Shi-ming, YUAN Fu-jin, WU Wen-rong, SHAN Gui-lan, CHEN Gong
    2020, 41(4):  36-39.  doi:10.12160/j.issn.1672-5190.2020.04.007
    Abstract ( 306 )   PDF (519KB) ( 62 )   Save
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    From 2012 to 2018, the degraded alpine meadow in Xiaozhongdian Town of Shangri-La City had been chosen and enclosed as test pastures. Aboveground biomass and botanical composition were observed and analyzed for both degraded and enclosed pastures. Redundancy theory, intermediate disturbance hypothesis and threshold value theory were used to evaluate the resistance, resilience, critical threshold and reasonable stocking capacity of the test pastures. The results showed that:(1) pastures enclosure could significantly (P<0.05) increase the aboveground biomass; due to the differences in ecological environment conditions at the test sites and basic situations of the pastures, the inter-annual fluctuation range and variations of the aboveground biomass were inconsistent, but its changing trend with enclosure duration exhibited an power function type (Y=aXb); (2) according to the changing trend of aboveground biomass, the resistance of the degraded alpine meadow in the test area was 250.4-518.8 g/m2, the pastures resilience was 54.7-68.0 months, and the critical threshold of pastures utilization was 420.6-588.7 g/m2; (3) suitable enclosure period for degraded alpine meadow was 2 to 5 years; continuous enclosure led to redundant growth of forage; the suitable stocking capacity of pastures after 5 years of enclosure was 1.1-3.4 sheep unit/hm2; after 7 years of continuous enclosure, non-feedable plants (including poisonous and harmful plants) in the pastures still accounted for 15.3% to 65.8%, and the suitable stocking capacity was low; (4) enclosure should be combined with measures such as weeds removing to reduce the redundancy of grass community components and to improve the stocking capacity of pastures.
    Effects of Different Temperature Treatments on Seed Germination of Three Species of Wild Leguminous Forages in Hulun Buir Natural Grassland
    XIAO Yan-zi, HUANG Xue-wen, Wurenqiqige, SUN Lin, DING Xia, HOU Mei-ling, ZHANG Pei-qing
    2020, 41(4):  40-44.  doi:10.12160/j.issn.1672-5190.2020.04.008
    Abstract ( 512 )   PDF (556KB) ( 80 )   Save
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    The objective of this study was to investigate the germination characteristics of wild forage seeds in Hulun Buir natural grassland. Germination tests of three species of wild leguminous forage seeds, including Medicago ruthenica, Astragalus laxmannii and Trifolium lupinaster collected from different areas, were carried out under different temperature conditions (15 ℃, 20 ℃, 25 ℃ and 30 ℃). The germination indicators of the selected forages were assessed and analyzed. The results showed that the seed germination rates of the three wild leguminous forages were generally low, and different temperatures had significant effects on germination of the seeds. The germination rate, germination energy, germination index and germination speed of Medicago ruthenica seeds and Trifolium lupinaster seeds were elevated with the increase of temperature, and the highest germination indicators were observed at 30 ℃; the germination rate, germination energy, germination index and germination speed of Astragalus laxmannii seeds were elevated at initial observation period and then reduced with the increase of temperature, and the highest germination indicators were found at 25 ℃. The highest seed vitality index (14.53) of Medicago ruthenica was observed at 20 ℃; the seed vitality index of Astragalus laxmannii ranged from 4.89 to 7.11 at different temperatures, and the highest value was found at 25 ℃; Trifolium lupinaster had the highest seed vitality index (18.04) at 30 ℃. Comprehensively, the suitable temperature for germination of the three species of wild leguminous forages was 25-30 ℃. The obtained results of this study provide a theoretical basis for improving germination rates of wild forage seeds in Hulun Buir natural grassland.
    Application and Research Advances on Fulvic Acid in Livestock and Poultry Production
    CHEN Yue-ji, YANG Na, SONG Zhi, LI Xian-yao
    2020, 41(4):  45-48.  doi:10.12160/j.issn.1672-5190.2020.04.009
    Abstract ( 339 )   PDF (563KB) ( 120 )   Save
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    Based on the research advances in recent years, this article introduces the functional characteristics and sources of fulvic acid as well as its application in production of pigs, dairy cattle, chickens and other livestock. Fulvic acid can promote appetite, improve health status, enhance disease resistance, improve production performance, feed utilization and carcass quality, and reduce ammonia emissions. The use of fulvic acid as a feed additive in green animal husbandry production has broad application prospect.
    Research Advances on Effects of Microorganisms on Composting Fermentation Process
    YUE Lin-fang, QIAO Jian-min, CHENG Li-xin, LI Yun-hua, Fengying, MA Wan-shan, Baohua, YU Zhao-hui, WANG Zhi-ming
    2020, 41(4):  49-54.  doi:10.12160/j.issn.1672-5190.2020.04.010
    Abstract ( 386 )   PDF (786KB) ( 170 )   Save
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    Composting is not only one of the most commonly used manure disposal methods in poultry and livestock farms, but also the most effective and economical method for treatment of solid organic waste at present. It is a process in which microorganisms convert waste organic matter into organic matter free of parasite eggs, pathogenic bacteria and odor under artificially controlled conditions. During the fermentation and decomposition of composting process, supplementation of beneficial microorganisms with tolerance to low or high temperature as well as strong cellulose-degrading ability can accelerate the composting process, improve the maturity effect, and reduce the toxicity of plants. This review summarizes the research and development status of composting microbial preparations in China and abroad as well as the types and application of composting microbial preparations used at low, medium and high temperature.
    Isolation and Identification of Lactic Acid Bacteria from Traditional Fermented Cream Products in Xilin Gol League, Inner Mongolia
    QIAO Jian-min, LI Zi-jian, WANG Li-mei, ZHANG Yuan-yuan, LI Xing-yun, Naqin, KANG Lian-he, ZHENG Wei, GUO Ke-xin, ZHAO Chen, WANG Xiao-dong
    2020, 41(4):  55-59.  doi:10.12160/j.issn.1672-5190.2020.04.011
    Abstract ( 297 )   PDF (983KB) ( 52 )   Save
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    In this study, the lactic acid bacteria were isolated and identified from two fermented cream samples collected in Zhenglan Banner, Xilin Gol League, Inner Mongolia. A total of 15 strains of lactic acid bacteria were obtained from these samples by catalase test, Gram staining, and molecular identification targeting 16S rDNA. All of the isolates were classified into the following four species within one genus: Lactobacillus parabuchnerin=1), Lactobacillus plantarumn=3), Lactobacillus brevisn=4) and Lactobacillus paracasein=7). The results obtained in this study provide theoretical basis and bacterial resources for the industrial production of traditional dairy products and for the research and development of excellent fermentation agents in Inner Mongolia.
    Analysis and Evaluation of Muscular Nutritional Components of Spinibarbus caldwelli under Artificial Breeding Condition in the Karst Mountainous Area of Guizhou Province
    LIANG Zheng-qi, ZHANG Long-ting, KUANG Hui-qi, XIAO Jia-cheng, QIN Yi-biao
    2020, 41(4):  60-63.  doi:10.12160/j.issn.1672-5190.2020.04.012
    Abstract ( 297 )   PDF (390KB) ( 60 )   Save
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    The aim of this study was to understand the muscular nutritional characteristics of Spinibarbus caldwelli under artificial breeding condition in the Karst mountainous area of Guizhou Province. The results showed that the contents of crude protein, crude fat, crude ash and water in fresh muscular samples of Spinibarbus caldwelli were 18.25%, 1.83%, 2.67% and 77.25%, respectively. A total of 18 kinds of amino acids were detected, accounting for 75.07% of the dry weight; among which, the taste-active amino acids accounted for 27.82% of the dry weight; ∑EAA/∑TAA and ∑EAA/∑NEAA were 40.68% and 68.58%, respectively. A total of 27 kinds of fatty acids were observed, among which, the saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) accounted for 24.18%, 53.31% and 22.51% of the total fatty acids, respectively. In conclusion, Spinibarbus caldwelli is a high-quality fish with superior nutritional value.
    Thoughts on Construction of Modern Animal Husbandry Production System in 33 Typical Animal Husbandry Banners (Counties) in Inner Mongolia
    GUO Tian-long, Jinhai, LI Chang-qing, ZHAI Xiu
    2020, 41(4):  64-68.  doi:10.12160/j.issn.1672-5190.2020.04.013
    Abstract ( 208 )   PDF (2079KB) ( 105 )   Save
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    According to the field investigation and industrial development analysis of 33 typical animal husbandry banners (counties) in Inner Mongolia, this paper proposes that, based on development dominated by herdsmen and driven by reform and innovation, the five major industries of grassland animal husbandry, including beef cattle industry, mutton sheep industry, camel industry, horse industry, and grass industry, should be prioritized, the integration of primary and secondary industries in pastoral areas should be guided and promoted, the development of tertiary industry should be encouraged and supported, the idea of constructing a modern animal husbandry production system with ′three guarantees′ and ′five supports′ should be persisted. Furthermore, we also put forward some targeted policy recommendations, in hoping to provide a reference for promoting the industrial modernization of pastoral areas in Inner Mongolia.
    Establishment of Tissue Culture and Cell Suspension Culture System of Cistanche deserticola Y.C. Ma
    HE Jiang-feng, WANG Li-wei, LIU Hong-kui, CHEN Xiu-juan, WANG Yun-hua, ZHAO Jie
    2020, 41(4):  69-76.  doi:10.12160/j.issn.1672-5190.2020.04.014
    Abstract ( 206 )   PDF (1229KB) ( 101 )   Save
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    In order to provide technical support for obtaining stable Cistanche deserticola Y.C. Ma resources, the succulent stems were used in this experiment as research materials to establish a stable cell suspension culture system of Cistanche deserticola Y.C. Ma via plant tissue culture technology and cell culture technology by inducing and sub-culturing of callus. The results showed that the best culture system for the callus of Cistanche deserticola Y.C. Ma was determined as follows: MS+PVP 2 000 mg/L + 2,4-D 2.0 mg/L + KT 0.5 mg/L + GA3 1.0 mg/L + sucrose 30 g + agar 7.0 g/L, the pH value was 5.8, and the culture conditions were lighting for 12 h/d at temperature of (25±1) ℃. The yellowish callus was a high-yielding cell line. The cell suspension culture system of Cistanche deserticola Y.C. Ma was determined as follows: B5 + GA3 2.0 mg/L + 6-BA 0.5 mg/L + 2,4-D 0.2 mg/L + sucrose 30 g/L, the pH value was 5.8, the culture conditions were lighting for 18 h/d at temperature of (25±1) ℃, and the shaker rotation speed was 110 r/min. The maximum value of cell dry weight (12.350 gDW/L) was obtained when the cell inoculation concentration was 4.0 gDW/L, which was the best culture system. This study has established a callus culture system and a stable cell suspension culture system of Cistanche deserticola Y.C. Ma.
    Effect of Glycocholic Acid(GCA)on Anti-oxidation Capacity of Mouse PeritonealMacrophages
    Wuyunfu, SHI Lin-lin, LI Pei-feng, GUAN Hong, YAO Qing-zhi, HE Xiu-ling
    2020, 41(4):  77-80.  doi:10.12160/j.issn.1672-5190.2020.04.015
    Abstract ( 163 )   PDF (4276KB) ( 55 )   Save
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    This study aimed to assess the antioxidative activity of glycocholic acid (GCA), one of the extracts from bile of cattle and sheep, on mouse peritoneal macrophages. A total of 5 experimental groups with mass concentrations of 1×10-3, 1×10-4, 1×10-5, 1×10-6 and 1×10-7 μg/mL of GCA were included. The mouse peritoneal macrophages were incubated with different concentrations of GCA for 24 hours, and the activities of glutathione peroxidase (GSH-Px)and superoxide dismutase (SOD), as well as the level of malondialdehyde (MDA), were determined according to the methods provided in the kit instructions. The results demonstrated that GCA reduced the level of MDA and elevated the activities of GSH-Px and SOD in mouse peritoneal macrophages, indicating its effective role in regulating the level of antioxidative stress in mouse peritoneal macrophages. In conclusion, GCA has the capacity to improve the antioxidative stress response.
    Research Advances on Pharmacological Actions of Flavonoids from Coreopsis tinctoria
    ZHANG Zhen-zhen, SHA Ai-long
    2020, 41(4):  81-84.  doi:10.12160/j.issn.1672-5190.2020.04.016
    Abstract ( 261 )   PDF (557KB) ( 71 )   Save
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    Coreopsis tinctorial contains flavonoids, coumarins, organic acids, amino acids, saponins, volatile oils, tannins, sugars, phenols and other chemical compositions. Among them, flavonoids are the dominant compositions of Coreopsis tinctoria with the highest content. Modern pharmacology researches have found that flavonoids have many pharmacological activities, such as anti-thrombosis, anti-aging, anti-diabetes, anti-tumor, anti-antibacterial, cardioprotective, lowing blood pressure, anti-oxidative, reducing blood lipid, enhancing immune function. In this paper, the pharmacological characteristics of Coreopsis tinctoria are reviewed, in hoping to provide references for the researchers with focus on flavonoids in Coreopsis tinctoria.
    Therapeutic Effect of Chaigui Cold Oral Liquid on Exogenous Wind-chill in Chickens
    YU Fang-xiao, LIU Ming-yan, XIAO Rui, SHI Jun, ZENG Yun, WEI Ying-yi, HU Ting-jun
    2020, 41(4):  85-89.  doi:10.12160/j.issn.1672-5190.2020.04.017
    Abstract ( 287 )   PDF (584KB) ( 59 )   Save
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    This study aimed to investigate the relationship between administrative dosage and therapeutic effect, evaluate the clinical therapeutic efficacy, and select the recommended dosage of Chaigui cold oral liquid. The exogenous wind-chill model was established with chickens. A total of 6 groups received different dosages of Chaigui cold oral liquid, a model control group, and a healthy control group were included in this study. There were 30 chickens in each group. After a continuous administration for 5 days, the chickens were observed persistently for 7 days. To determine the optimal administrative dosage, the clinical efficacy of different dosages of Chaigui cold oral liquid was comprehensively evaluated via the indicators of effective rate, curative rate and weight gain rate. The results showed that after 5 days treatment, the total effective rate, curative rate and weight gain rate of the chickens received the administration of 1.0 mL/L of Chaigui cold oral liquid were 93.33%, 76.67% and 223.40%, respectively, and those of the chickens received the administration of 8.0 mL/L of the liquid were 100%, 86.67% and 319.75%, respectively. Taking the factors of effective rate, curative rate, clinical manifestations, average weight gain and treatment cost into consideration, the clinical medication method of Chaigui cold oral liquid for exogenous wind-chill in chickens is as follows: adding 1.0 mL of the liquid in every 1 L of drinking water for continuous 3 to 5 days.
    Research Advances on Biological Activities and Application of Saponins
    WEN Li-wei, ZHANG Qiong-lin, Qinggele, JIA Shu-ping, JI Peng-hua, CHEN Qiang, WANG Hai-xia, SUN Jie
    2020, 41(4):  90-96.  doi:10.12160/j.issn.1672-5190.2020.04.018
    Abstract ( 347 )   PDF (932KB) ( 132 )   Save
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    Saponins have various biological activities such as anti-tumor, improving immunity, and regulating lipid metabolism. With the continuous development of separation and purification technology, understandings on the biological activity, research value, developmental prospects of saponins have become more clear. This paper reviews the structure and biological activity as well as application value in animal / plant production and drug development of saponins, in hoping to provide scientific reference for further understanding, research, development and utilization of saponins.
    Application of TLC and HPLC Methods in Quality Control of Traditional Chinese Veterinary Medicines
    LIANG Yong, HUANG Chen, JIN Li-qin, LIU Meng-bing, FANG Meng
    2020, 41(4):  97-100.  doi:10.12160/j.issn.1672-5190.2020.04.019
    Abstract ( 175 )   PDF (493KB) ( 81 )   Save
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    The quality control of traditional Chinese veterinary medicines has always been the focus for the researchers of traditional Chinese veterinary medicine. At present, the chromatographic technology research is developing rapidly in China, and its roles in quality control of traditional Chinese veterinary medicines have been becoming more and more important. The application of thin layer chromatography (TLC) and HPLC (high performance liquid chromatography) methods in quality control of traditional Chinese veterinary medicines in recent years has been sorted out and reviewed, in hoping to provide effective references for the detection of traditional Chinese veterinary medicines.
    Antibacterial Effects of Seven Kinds of Mongolian Medicines Against Escherichia coli and Staphylococcus aureus in Vitro
    Amuguleng, LI Zi-jian, PEI Le, LI Feng, GENG Wan-heng, WANG Feng-wu, HOU Yong-yue, LIU Xiu-li
    2020, 41(4):  101-106.  doi:10.12160/j.issn.1672-5190.2020.04.020
    Abstract ( 247 )   PDF (1424KB) ( 59 )   Save
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    This study aimed to screen the Mongolian medicines that have good antibacterial effects against Staphylococcus aureusS. aureus) and Escherichia coliE. coli). The hole-punch method was employed to determine the susceptibility of S. aureus and E. coli to pomegranate rind, Terminalia chebula, Artemisia austriaca, Scutellaria baicalensis, Astragalus mongholicus, Sophora flavescens, and Clematis florida. The minimal inhibitory concentration (MIC) of the seven kinds of Mongolian medicines against S. aureus and E. coli were assessed by using micro-dilution method. Furthermore, the fractional inhibitory concentration index (FICI) of the screened Mongolian medicines that performed good antibacterial effects against S. aureus and E. coli were evaluated via chessboard method. The results showed that the inhibitory zone diameter of pomegranate rind, Terminalia chebula and Scutellaria baicalensis against S. aureus ranged from 23.30 to 32.60 mm, and corresponding MICs ranged from 0.98 to 7.80 mg/mL; the inhibitory zone diameter of pomegranate rind, Terminalia chebula and Scutellaria baicalensis against E. coli ranged from 5.20 to 12.50 mm, and corresponding MICs ranged from 7.80 to 31.50 mg/mL. The FICI of combinative use of pomegranate rind and Terminalia chebula against S. aureus was 1.00, indicating an additive effect; the FICIs of combinative use of Terminalia chebula and Scutellaria baicalensis as well as Terminalia chebula and pomegranate rind against E. coli were both 0.75, indicating an additive effect. In summary, from the perspective of comprehensive antibacterial effects, S. aureus and E. coli are extremely sensitive or highly sensitive to pomegranate rind, Terminalia chebula and Scutellaria baicalensis; pomegranate rind, Scutellaria baicalensis and Terminalia chebula can be given priority in formulas that inhibit S. aureus, while Scutellaria baicalensis, Terminalia chebula and pomegranate rind can be given priority in formulas that inhibit E. coli.
    Comparison of Intestinal Microbial Diversity Between Piglets with or without Diarrhea
    Terigele, BAI Xue, DUAN Shi, LIU Na-na, SUN Yan-yong, FU Shao-yin, LIU Yong-bin, WU Rui-jun, ZHAO Pei-ting, ZHANG Wen-guang
    2020, 41(4):  107-110.  doi:10.12160/j.issn.1672-5190.2020.04.021
    Abstract ( 279 )   PDF (1039KB) ( 122 )   Save
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    [Objective] To assess the influence of diarrhea on intestinal microbial diversity of nursery piglets by comparing the difference in fecal flora between piglets with or without diarrhea. [Method] The fecal samples of piglets in the diarrhea group (n=6) and healthy group (n=3) were sequenced by using 16S rDNA high-throughput sequencing technology, and the composition and structure of the intestinal microbial community of the two groups were compared. [Result] The fecal flora between the diarrhea group and the healthy group was significantly (P<0.05) different, and the intestinal flora diversity of the diarrhea group was significantly (P<0.05) lower than that of the healthy group. Compared with the healthy group, at the phylum level, the relative abundance of Fusobacteria and Proteobacteria of the diarrhea group was increased significantly (P<0.05), while that of Firmicutes was decreased significantly (P<0.05); at the genus level, the relative abundance of Fusobacterium, Prevotella, Escherichia, Lactobacillus, Anaerovibrio, Alloprevotella and Fusobacteriaceae_unclassified of the diarrhea group was increased significantly (P<0.05), while that of most genus belonging to Firmicutes was decreased significantly (P<0.05). [Conclusion] The analysis and comparison of the intestinal microbial diversity between diarrhea piglets and healthy piglets provide a theoretical basis for the prevention and treatment of diarrhea in nursery piglets.
    Application Status and Research Prospects of Salmonella Vaccines
    ZHANG Jun-xia, FENG Yong-miao, LI Rong, WANG Li
    2020, 41(4):  111-114.  doi:10.12160/j.issn.1672-5190.2020.04.022
    Abstract ( 263 )   PDF (485KB) ( 104 )   Save
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    Salmonellosis is a zoonotic disease caused by bacterial infection of the Salmonella genus. Vaccination is an effective way to prevent and control salmonellosis in livestock and poultry. Due to the high cost, unstable immune effect and complicated immune procedure, the application of the currently used Salmonella attenuated vaccines is limited. Oral plant- based Salmonella vaccines have the advantages of high yield, short production time, low cost and high safety. This paper reviews the research prospects of oral plant-based Salmonella vaccines, in hoping to provide references for the further research, development and widespread application of this type of vaccines.
    Teaching Reform and Exploration of Practical Genetic Testing Technology for Aquaculture Major
    ZHAO Yi-ping, DU Ming, LI Bei, BAI Dong-yi, Dugarjaviin Mang-Lai
    2020, 41(4):  115-117.  doi:10.12160/j.issn.1672-5190.2020.04.023
    Abstract ( 165 )   PDF (448KB) ( 55 )   Save
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    Practical Genetic Testing Technology is an indispensable experimental course for aquaculture major, and it is the main way for undergraduates to understand and master the basic theory and experimental technology of genetics. On the basis of analyzing existing problems of the experimental teaching of this course, the teaching effect and quality was improved by adding experimental objects, introducing new problem-based learning (PBL) teaching method, optimizing the evaluation and assessment methods, and strengthening cultivation of teachers. The teaching reform and exploration of Practical Genetic Testing Technology mentioned above provides practical experience for the agricultural colleges and universities.
    Observation on Bleaching and Cementing Effect in Preparation of Rabbit Bone Specimens
    WANG Chao, LV Xu, MAO Fei, SHAO Liang-yong, LIU Tian, FAN Xu, WEI Zi-heng, LU Ze-kai, XU Wen-jing, WANG Cai-yun
    2020, 41(4):  118-121.  doi:10.12160/j.issn.1672-5190.2020.04.024
    Abstract ( 1169 )   PDF (590KB) ( 65 )   Save
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    In the process of rabbit bone specimens preparation, bleaching and cementing are very important steps which directly affect the overall quality and appearance of the specimens. In order to find out satisfied bleaching and cementing process for rabbit bone specimens, a total of 8 degreased rabbit bone samples were randomly divided into 4 groups and bleached with 5%, 15%, 25% and 30% hydrogen peroxide solutions, respectively. After bleaching, the rabbit bone samples were randomly divided into four groups of A, B, C and D. Using unmovable joints, semi-movable joints and movable joints as experimental subjects, the cementing effect of four adhesives, including bone glue adhesive, AB glue, 502 glue and hot melt adhesive, were observed and compared. The results showed that the hydrogen peroxide concentrations and treatment times required for bones in varied parts were different: skull was bleached in 30% hydrogen peroxide for 1.5 h, cervical vertebra, lumbar vertebra and sternum were bleached in 25% hydrogen peroxide for 1.5 h; costal bone, humerus and scapula were bleached in 15% hydrogen peroxide for 1.5 h; metacarpal bone, coccygeal vertebra and phalanx and some other bones were bleached in 5% hydrogen peroxide for 2 h. Furthermore, bone glue adhesive had quick effect, tight bonding, high strength, and good shaping effect. The bleaching and cementing process determined in this study can effectively reduce the damage to the rabbit bones during the specimens preparation, keep the original appearance of the bones to the greatest extent, and make the specimens more vivid.
    Teaching Reform and Practice of Livestock Breeding
    ZHANG Jing-feng, ZHAO Jin-yan, LI Wan-tao, NIU Hui, ZHAO Guang-wei, ZHANG Chang-xing
    2020, 41(4):  122-124.  doi:10.12160/j.issn.1672-5190.2020.04.025
    Abstract ( 141 )   PDF (457KB) ( 35 )   Save
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    In order to meet the requirements for the application-oriented undergraduate talents training in university, to improve undergraduate′ professional quality and professional knowledge application ability, reforms on the traditional teaching methods, teaching means and teaching contents of Livestock Breeding are conducted. By exploring the teaching methods and models suitable for application-oriented talents training, undergraduates′ interest in learning was stimulated, classroom teaching effects and undergraduates′ practical ability were improved. The purpose of this teaching reform and practice is to lay a solid professional foundation for undergraduates′ participation in the industry work after graduation.
    Discussion on Effective Ways for Training Application-oriented Talents in Animal Science Major
    WANG Qiu-ju, CUI Yi-zhe, WEI Chun-bo, ZHANG Ai-zhong, LIU Sheng-jun, GUO Li
    2020, 41(4):  125-128.  doi:10.12160/j.issn.1672-5190.2020.04.026
    Abstract ( 163 )   PDF (598KB) ( 90 )   Save
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    The sustainable development of animal husbandry requires the participation of a large number of skilled talents who have mastered the latest production technology and processes of the enterprise. Therefore, in the teaching of animal science major, it is necessary to highlight the practicality and pertinence, to select the teaching contents in a targeted manner according to the needs of employment, and to scientifically construct curriculum framework with strong pertinence and capability to cultivate high-level practical talents. In this way, the teaching contents can be closely linked with the progress of the industry, and the application-oriented talents urgently needed by the industry can be effectively cultivated. This paper discusses the establishment of training goal for application-oriented talents in animal science major in agricultural colleges and universities, and expounds the reform measures taken to achieve this goal, in hoping to provide insights and strategies for cultivating application-oriented talents in animal science major in new situation.

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(Published monthly,Science 1973)
Responsible Unit:Inner Mongolia Department of Agriculture and Animal Husbandry
Sponsor Unit:Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences
International standard serial number:ISSN 1672-5190
Domestic unified serial number:CN 15-1228/S
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