鄱阳湖周边猪场废水中mcr基因及ESKAPE病原体的污染特征

doi:10.12160/j.issn.1672-5190.2025.05.001

Abstract

Abstract: [Objective] To investigate the contamination characteristics of ESKAPE pathogens and the mobilized colistin resistance gene （mcr gene） in pig farm wastewater surrounding Poyang Lake. [Methods] Nineteen wastewater samples were collected from 11 pig farms near Poyang Lake. Metagenomic sequencing technology was used to determine the relative abundance of ESKAPE pathogens and mcr genes, followed by Pearson correlation analysis. [Results] A total of 26 types of mcr genes and mutants were detected in the 19 wastewater samples, with 8 to 19 types detected per sample; five ESKAPE pathogens were detected, including Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, and Enterococcus faecium. Comparing the removal rates of mcr genes before and after wastewater treatment showed that 12 types of mcr genes decreased in all farms where removal rates could be calculated （removal rates 56.0%-100%） and 12 other genes （excluding mcr-4.3 and mcr-3.6） decreased in some farms but increased in others （increase rates 22.2%-8,500.0%）. Both P. aeruginosa and K. pneumoniae showed a significant positive correlation with the mcr-2 and mcr-3 genes （P<0.05）. [Conclusion] ESKAPE pathogens and mcr gene contamination are present in pig farm wastewater surrounding Poyang Lake, and Gram-negative ESKAPE pathogens may carry mcr genes. The existing wastewater treatment methods are insufficient to effectively remove mcr genes, thereby increasing the environmental health risks associated with pig farm wastewater. The findings provide a reference for strengthening manure management at pig farms near Poyang Lake and improving the quality of the farming ecological environment.

Key words: mcr gene, ESKAPE pathogens, resistance gene, pig farm wastewater, surrounding area of Poyang Lake

CLC Number:

S8521.2
X713

YANG Lili, XIAO Guangming, WANG Zhong, WANG Wang, WANG Liqi. Contamination Characteristics of mcr Genes and ESKAPE Pathogens in Pig Farm Wastewater Surrounding Poyang Lake[J]. Animal Husbandry and Feed Science, 2025, 46(5): 1-9.

References

[1] BROVEDAN M,MARCHIARO P M,MORÁN-BARRIO J,et al.Complete sequence of a bla(NDM-1)-harboring plasmid in an Acinetobacter bereziniae clinical strain isolated in Argentina[J].Antimicrobial Agents and Chemotherapy,2015,59(10):6667-6669.
[2] DUBASHYNSKAYA N V,SKORIK Y A.Polymyxin delivery systems:recent advances and challenges[J].Pharmaceuticals,2020,13(5):83.
[3] EL-SAYED AHMED M A E,ZHONG L,SHEN C,et al.Colistin and its role in the Era of antibiotic resistance:an extended review (2000—2019)[J].Emerging Microbes Infections,2020,9(1):868-885.
[4] 李立,叶璟,胡蕾,等.多黏菌素用法及药代动力学研究进展[J].药学与临床研究,2021,29(6):454-457,461.
[5] LIU Y Y,WANG Y,WALSH T R,et al.Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China:a microbiological and molecular biological study[J].The Lancet Infectious Diseases,2016,16(2):161-168.
[6] 马芙蓉. 沙门氏菌多黏菌素耐药基因mcr-1的流行病学及耐药传播机制研究[D].广州:广州医科大学,2020.
[7] AZEVEDO L,TACÃO M,SILVA A R,et al.Surveillance of plasmid-mediated mcr-1,mcr-3,mcr-4 and mcr-5 genes in human isolates,in Aveiro,Portugal[J].International Journal of Infectious Diseases,2020,101:343.
[8] CHERAK Z,LOUCIF L,MOUSSI A,et al.Epidemiology of mobile colistin resistance (mcr) genes in aquatic environments[J].Journal of Global Antimicrobial Resistance,2021,27:51-62.
[9] 曹振华,张媛,马奔,等.南京地区污水厂、自来水厂及长江中抗性基因MCR-1和NDM-1的污染特征[J].环境科学研究,2019,32(3):406-414.
[10] 伍盛鋆. 猪场环境中mcr-1的分布特征及在不同菌种间传播的初步研究[D].广州:华南农业大学,2017.
[11] SHEN C,FENG S Y,CHEN H T,et al.Transmission of mcr-1-producing multidrug-resistant Enterobacteriaceae in public transportation in Guangzhou,China[J].Clinical Infectious Diseases,2018,67(suppl_2):S217-S224.
[12] RICE L B.Federal funding for the study of antimicrobial resistance in nosocomial pathogens:no ESKAPE[J].The Journal of Infectious Diseases,2008,197(8):1079-1081.
[13] FOUNOU R C,FOUNOU L L,ESSACK S Y.Clinical and economic impact of antibiotic resistance in developing countries:a systematic review and meta-analysis[J].PLoS One,2017,12(12):e0189621.
[14] NAVIDINIA M,GOUDARZI M,RAMESHE S M,et al.Molecular characterization of resistance genes in MDR-ESKAPE pathogens[J].Journal of Pure and Applied Microbiology,2017,11(2):779-792.
[15] 楼倩,韩柳,何涛,等.鄱阳湖流域锦江抗生素和抗性基因的赋存特征、时空分布及来源分析[J].生态毒理学报,2025,20(3):233-252.
[16] YANG Z W,CHEN H T,MA C,et al.A novel framework reveals anthropogenic stressors of phosphorus polluted river-lake connection water system in Poyang lake basin of China[J].Journal of Environmental Management,2024,370:122794.
[17] 王惠明,张鸿燕,陈燕,等.江西省畜禽养殖业污染状况及预防对策[J].安徽农业科学,2016,44(32):59-62.
[18] LING Z R,YIN W J,SHEN Z Q,et al.Epidemiology of mobile colistin resistance genes mcr-1 to mcr-9[J].Journal of Antimicrobial Chemotherapy,2020,75(11):3087-3095.
[19] WANG C C,FENG Y,LIU L N,et al.Identification of novel mobile colistin resistance gene mcr-10[J].Emerging Microbes and Infections,2020,9(1):508-516.
[20] 张雪,谢小芳,王敏,等.碳青霉烯类耐药肠杆菌科细菌多黏菌素耐药性调查及分子机制分析[J].临床检验杂志,2020,38(8):592-596,602.
[21] 陈秋兰. LC-MS/MS法测定沉积物中磺胺类和四环素类抗生素[J].环境监测管理与技术,2019,31(3):49-52.
[22] 秦兴秀,杨敏娜,王来梁.固相萃取-UPLC-MS/MS法测定环境水体中多种抗生素[J].环境监测管理与技术,2024,36(3):51-54.
[23] 别又才,何国戈,葛影影,等.中国生猪养殖业粪污综合处理模式及展望[J].农业展望,2021,17(2):104-108.
[24] 杨永青,许继飞,董泰音,等.水体和土壤环境中抗生素抗性基因(ARGs)的污染特征和消除[J].北方农业学报,2018,46(3):76-82.
[25] DAVIES J,DAVIES D.Origins and evolution of antibiotic resistance[J].Microbiology and Molecular Biology Reviews,2010,74(3):417-433.
[26] 张治国,李斌绪,李娜,等.污水深度处理工艺对抗生素抗性菌和抗性基因去除研究进展[J].农业环境科学学报,2018,37(10):2091-2100.
[27] 张瑞泉,应光国,丁永祯,等.广东西枝江-东江流域抗生素抗性基因污染特征研究[J].农业环境科学学报,2013,32(12):2471-2479.
[28] DENISSEN J,REYNEKE B,WASO-REYNEKE M,et al.Prevalence of ESKAPE pathogens in the environment:Antibiotic resistance status,community-acquired infection and risk to human health[J].International Journal of Hygiene and Environmental Health,2022,244:114006.
[29] DE OLIVEIRA D M P,FORDE B M,KIDD T J,et al.Antimicrobial resistance in ESKAPE pathogens[J].Clinical Microbiology Reviews,2020,33(3):e00181-19.
[30] SATILMIS L,VANHEMS P,BÉNET T.Outbreaks of vancomycin-resistant enterococci in hospital settings:a systematic review and calculation of the basic reproductive number[J].Infection Control and Hospital Epidemiology,2016,37(3):289-294.
[31] SOOD G,PERL T M.Outbreaks in health care settings[J].Infectious Disease Clinics of North America,2021,35(3):631-666.
[32] LUO Q X,LU P,CHEN Y B,et al.ESKAPE in China:epidemiology and characteristics of antibiotic resistance[J].Emerging Microbes and Infections,2024,13(1):2317915.
[33] WYRES K L,LAM M M C,HOLT K E.Population genomics of Klebsiella pneumoniae[J].Nature Reviews Microbiology,2020,18(6):344-359.
[34] MÜLLER C,REUTER S,WILLE J,et al.A global view on carbapenem-resistant Acinetobacter baumannii[J].mBio,2023,14(6):e0226023.
[35] TENOVER F C,NICOLAU D P,GILL C M.Carbapenemase-producing Pseudomonas aeruginosa-an emerging challenge[J].Emerging Microbes and Infections,2022,11(1):811-814.
[36] DOIJAD S P,GISCH N,FRANTZ R,et al.Resolving colistin resistance and heteroresistance in Enterobacter species[J].Nature Communications,2023,14(1):140.
[37] SHRESTHA P,COOPER B S,COAST J,et al.Enumerating the economic cost of antimicrobial resistance per antibiotic consumed to inform the evaluation of interventions affecting their use[J].Antimicrobial Resistance and Infection Control,2018,7:98.
[38] RUSSO T P,MINICHINO A,GARGIULO A,et al.Prevalence and phenotypic antimicrobial resistance among ESKAPE bacteria and enterobacterales strains in wild birds[J].Antibiotics,2022,11(12):1825.
[39] GU C C,LI X W,ZOU H Y,et al.Clonal and plasmid-mediated dissemination of environmental carbapenem-resistant Enterobacteriaceae in large animal breeding areas in northern China[J].Environmental Pollution,2022,297:118800.
[40] AL BAYSSARI C,DABBOUSSI F,HAMZE M,et al.Emergence of carbapenemase-producing Pseudomonas aeruginosa and Acinetobacter baumannii in livestock animals in Lebanon[J].Journal of Antimicrobial Chemotherapy,2015,70(3):950-951.

Contamination Characteristics of mcr Genes and ESKAPE Pathogens in Pig Farm Wastewater Surrounding Poyang Lake

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