Abstract: Protein is an essential nutrient for animal. For non-ruminant animals,protein nutrition is virtual amino acid/small peptide nutrition. Because of the special digestive physiological stucture and microbial digestion for ruminant, the protein nutrition is more complicated than non-ruminant animals. The evaluation technology for the nutritive value of protein is the basis and prerequisite for studying the protein nutrition of animals. Aim at the digestive physiological characteristics of ruminant, some valuation technologies for the nutritive value of protein in ruminant like nylon bag technology, microbial labeling technique, isotopic method, purine derivatives and artificial rumen, etc. were reviewed in this paper. This review is helpful to theoretical research and productive practice.