Abstract: The demonstration,popularization and application works of probiotics preparation in the production of laying breeder hens was done,in order to promote the wide application of probiotics preparation in the production of laying breeder hens,ensure the green and safe production of breeder poultry in suburbs of Beijing and increase the economic and social benefits.The popularization results showed that adding probiotics preparation to feed can increase the laying rate of laying hens and the qualified rate of breeding eggs,decrease the feed conversion and the rate of dead bird,improve the fertilization rate of hatching eggs,the hatchability of fertile eggs and the rate of healthy chicken and decrease the rate between breeding eggs and female chickens.It has some economic benefits and it is a kind of green and safe production model,so it can be and popularized and used in laying breeder hens.