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Insights from the Dutch Dairy Product Safety Supervision System for China
SUN Yanqiu, HUANG Yaping
Animal Husbandry and Feed Science    2025, 46 (1): 61-71.   DOI: 10.12160/j.issn.1672-5190.2025.01.009
Abstract67)      PDF(pc) (1349KB)(756)       Save
The dairy industry is an important part of the animal husbandry industry, and the dairy product industry chain is one of the most complex food industry chains. Dairy product safety cannot be ignored and is a global issue. Through long-term practice, the Netherlands has established a relatively comprehensive dairy product safety supervision system. Based on this, this paper comprehensively analyzes the regulatory advantages of the Dutch dairy product industry chain from the development status and characteristics of the Dutch dairy industry and the food supervision system. Furthermore, it proposes development recommendations for the supervision of food safety in China′s dairy industry from three aspects: the quality of raw milk, regulatory authorities, and financial support, aiming to promote the high-quality development of dairy product safety supervision in China.
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Survey on the Current Situations of Prepacked Children's Food Sold in Ordos City of Inner Mongolia
Animal Husbandry and Feed Science    2014, 35 (11): 89-89.   DOI: 10.12160/j.issn.1672-5190.2014.11.039
Abstract95)      PDF(pc) (396KB)(437)       Save
The brands and varieties of prepacked children's food in 50 supermarkets and stores in Dongsheng District and three surrounding banners in Ordos City of Inner Mongolia were investigated. The results showed that there were 169 brands, 222 kinds of children's food in Ordos. Among them, 49 brands (29.0%), 61 kinds(27.5%)were nutritious or nutrient supplement types. 120 brands(71.0%), 161 kinds(72.5%) were leisure food or even junk food. There were 124 brands(62.3%), 180 kinds(59.4%) of children's food sold in Dongsheng District of Ordos City. There were 75 brands(37.7%), 123 kinds(59.4%)of children's food sold in three surrounding banners. These indicated that the economic and culture development levels in different regions were different, and the kinds and levels of children's food in different regions were different. The information about package and label was overall and the food in the survey had QS label. The labels of children's food in the survey were more qualified than that in the past years, but their quality needed to be detected.
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High-throughput Sequencing Technology and Its Application in Life Science
Animal Husbandry and Feed Science    2012, 33 (2): 25-25.   DOI: 10.12160/j.issn.1672-5190.2012.02.012
Abstract279)      PDF(pc) (361KB)(506)       Save
Sanger sequencing method, first-generation sequencing method, has provided a powerful tool for human to decipher genetic codes. After more than 30 years′ continual improvement, second-generation sequencing method with some main characteristics of high throughput, rapid sequence and low cost has developed, and it brings revolutionary changes for life science field. The differences between second-generation sequencing and Sanger sequencing method were contrasted in this paper, and the application of second-generation sequencing in life science research was described.
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Construction of Knowledge Graph for Animal Husbandry Laws and Regulations in China
ZHANG Rongzhen, MENG Xiaoyan, LIU Xiaoxiao, WANG Yang
Animal Husbandry and Feed Science    2023, 44 (3): 69-74.   DOI: 10.12160/j.issn.1672-5190.2023.03.010
Abstract174)   HTML8)    PDF(pc) (945KB)(426)       Save

[Objective] The present study was conducted to propose a method for organizing the knowledge of animal husbandry laws and regulations to achieve a more orderly organization of relevant knowledge. [Method] The online legal resources in the websites of flk.npc.gov.cn, pkulaw.com, lawyee.org, wkinfo.com.cn, and law.cnki.net were used as the data sources of animal husbandry laws and regulations, the relevant laws and regulations of animal husbandry were screened and obtained. Subsequently, the corresponding formulation authority, timeliness, effectiveness hierarchy, regulation category, issuing date, implementation date and year information were extracted, and the obtained data was preprocessed. The ontology was built, and the entities and relationships were defined. Using rule-based method, the entities and relationships from the obtained laws and regulations texts were extracted, and then temporarily stored in MySQL database. The SQLAlchemy database and py2neo database in Python were used to operate MySQL database and Neo4j Graph database to convert the data in MySQL into entity relationship triad, and then stored in Neo4j Graph database to complete the storage of knowledge graph of animal husbandry laws and regulations. [Result] A total of 287 laws and regulations related to animal husbandry were collected and classified based on whether the laws and regulations contained chapters. Among these, 211 laws and regulations contained chapters, totaling 1 470 chapters and 10 457 articles, whereas 76 laws and regulations did not contain chapters, totaling 2 145 articles. There were 10 types of entities defined, including ′laws and regulations′, ′issuing authority′, ′timeliness′, ′effectiveness hierarchy′, ′regulation category′, ′chapter′, ′article′, etc. There were 10 types of relationships defined, including ′the relationship of animal husbandry laws and regulations to category of laws and regulations ′, ′the relationship of animal husbandry laws and regulations to timeliness of laws and regulations′, ′the relationship of animal husbandry laws and regulations to issuing authority of laws and regulations′, and ′the relationship of animal husbandry laws and regulations to effectiveness hierarchy of laws and regulations′, etc. A knowledge graph of animal husbandry laws and regulations covering 14 936 entities and 16 339 relationships was constructed. [Conclusion] This method can be applied to organize the knowledge on animal husbandry laws and regulations, making the organization of knowledge more systematic and closely related.

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Animal Husbandry and Feed Science    2009, 30 (3): 37-37.   DOI: 10.12160/j.issn.1672-5190.2009.03.016
Abstract153)      PDF(pc) (195KB)(1265)       Save
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Research Progress on Laboratory Diagnosis Methods of Infectious Etiology of Calf Diarrhea
Animal Husbandry and Feed Science    2015, 36 (1): 90-90.   DOI: 10.12160/j.issn.1672-5190.2015.01.040
Abstract111)      PDF(pc) (458KB)(403)       Save
Diarrhea is a common disease in calf rearing. Serious diarrhea always causes death of calf and brings huge economic loss to dairy cow breeding industry worldwide. While the emergence and prevalence of calf diarrhea was controlled in some of the dairy farms to some extent as the increase of feeding level, the calf diarrhea is still hard to be completely controlled due to the numerous infectious etiologies and the complex infection situation. The research progress on laboratory diagnosis methods of infectious etiology of calf diarrhea was reviewed in this paper, with the hope to provide theoretical foundation for the diagnosis, prevention and treatment of calf diarrhea.
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Animal Husbandry and Feed Science    2008, 29 (5): 87-87.   DOI: 10.12160/j.issn.1672-5190.2008.05.047
Abstract375)      PDF(pc) (148KB)(496)       Save
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Animal Husbandry and Feed Science    2009, 30 (11): 19-19.   DOI: 10.12160/j.issn.1672-5190.2009.11.012
Abstract97)      PDF(pc) (420KB)(497)       Save
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Animal Husbandry and Feed Science    2012, 33 (5): 155-155.   DOI: 10.12160/j.issn.1672-5190.2012.05.086
Abstract149)      PDF(pc) (184KB)(258)       Save
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Overview of Research on the Inducements and Treatment Technologies of Sleepwalking
Animal Husbandry and Feed Science    2013, 34 (12): 94-94.   DOI: 10.12160/j.issn.1672-5190.2013.12.046
Abstract128)      PDF(pc) (313KB)(241)       Save
On the basis of extensive literature retrieval, the studies on the inducements and treatment technologies of sleepwalking were summarized, so as to provide scientific materials for the health care of people.
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Progress in Research on the Iron Amino Acid Chelate and Its Application
Animal Husbandry and Feed Science    2012, 33 (7): 17-17.   DOI: 10.12160/j.issn.1672-5190.2012.07.007
Abstract382)      PDF(pc) (278KB)(348)       Save
Iron is one of the essential trace elements for the life activities of human and animals. It participates in the biosynthesis and metabolism of hemoglobin,myoglobin and many kinds of oxidases and it is closely related with enhancing the organism immunity, increasing animal' s reproductivity and growth indicators . As a kind of high-quality organic iron source, iron amino acid chelate was paid attentions to because of its advantages (high absorption rate, high biological efficiency and little pollution to the environment and so on). The absorption and metabolism in vivo, physiological functions of iron amino acid chelate and its applications in the practice were elaborated, in order to provide references for the related researches.
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Research Progress and Development Trend of Feed Testing in China:Based on CiteSpace Visual Analysis
LIU Jiahui
Animal Husbandry and Feed Science    2024, 45 (6): 49-57.   DOI: 10.12160/j.issn.1672-5190.2024.06.008
Abstract109)      PDF(pc) (1712KB)(347)       Save
[Objective] This study aims to comprehensively analyze the development history, research hotspots, and trends of feed testing in our country while providing visual data support. [Method] Utilizing the China National Knowledge Infrastructure (CNKI) database as the data source, this study retrieved journal literature on feed detection research in China from 1993 to 2024. The search themes included feed detection, feed detection technology, feed quality detection, and feed detection analysis. Additionally, an analysis of the annual publication volume in this research field was conducted. We employed bibliometric visualization software of CiteSpace 6.3.3 and VOSviewer 1.6.20 to analyze highly cited authors, cooperative networks, co-occurring keywords, and important journals, systematically identifying the research hotspots and trends of feed testing. [Results] Based on the predefined retrieval criteria, a total of 550 journal articles were included in this study. The peak publication year was 2009, during which 48 articles were published. The keyword cluster analysis reveals that preservatives and pig feed are the most prominent research foci in the domain of feed testing in China. Furthermore, preservatives, sensors, Shigella, and tetracycline have emerged as key topics of interest. The analysis of highly cited authors and their collaboration networks reveals that Jia Tao, Li Danni, and Gao Wenhui rank as the top three authors in terms of citation frequency of research outcomes, and they have also established the three largest collaborative networks. Additionally, Liu Muhua has made substantial contributions to the most recent research advancements. The co-occurrence keyword network analysis reveals that research in this field has evolved through three distinct stages: initial focus on toxin testing, subsequent exploration of detection methods and components, and the current emphasis on the application of emerging technologies and advanced detection methodologies. Looking ahead, pig feed testing, emerging technologies and testing methods, new feed testing, and are anticipated to be future research hotspots. [Conclusion] Through bibliometric analysis, we have identified the key research hotspots and emerging trends in the field of feed testing in China.
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Animal Husbandry and Feed Science    2013, 34 (3): 85-85.   DOI: 10.12160/j.issn.1672-5190.2013.03.043
Abstract196)      PDF(pc) (253KB)(311)       Save
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Research Advance on the Detection Technology of Cryptosporidiosis
Animal Husbandry and Feed Science    2009, 30 (1): 41-41.   DOI: 10.12160/j.issn.1672-5190.2009.01.022
Abstract178)      PDF(pc) (313KB)(187)       Save
Cryptosporidium was an important pathogen of animal and human diarrhea and one of main lethal factors in AIDS patient. At present, the research on Cryptosporidiosis had become a focus in the research field of global parasitology. There was no efficient drug till now, which could cure Cryptosporidiosis, so the fast, simple, convenient and accurate discovery of Cryptosporidium was the key for con trolling Cryptosporidiosis. As the molecular biotechnology was applied, the detection technology of Cryptosporidiosis got very great development. Now, the detection technologies of Cryptosporidiosis in recent years were summed up simply.
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Research Progress on Secondary Metabolites of SteUera chamaejasme
Animal Husbandry and Feed Science    2016, 37 (8): 35-35.   DOI: 10.12160/j.issn.1672-5190.2016.08.011
Abstract138)      PDF(pc) (351KB)(505)       Save
Stellera chamaejasme, belonging to Thymelaeaceae, is widely distributed in grassland and is highly toxic for bovine, sheep and other domestic animals. The mistakenly eating of Stellera chamaejasme commonly results in diarrhea and even death of animals. However, the secondary metabolites of Stellera chamaejasme exhibit strong insecticidal and bactericidal activity, which has the potential to develop as green pesticide products. Here, we introduced the basic characteristics, geographical distribution, and ecological harm to the environment of Stellera chamaejasme, reviewed the medicinal value and status of research, development and utilization of Stellera chomo~jasme, described the allelopathy of Stellera chamaejasme, and prospected the application of SteUera chamaejasme, so as to provide a theoretical basis for achieving the application value of SteUera chamaejasme in the aspects of green pesticide development and environmental protection.
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Animal Husbandry and Feed Science    2013, 34 (12): 84-84.   DOI: 10.12160/j.issn.1672-5190.2013.12.040
Abstract97)      PDF(pc) (167KB)(273)       Save
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Effect of Dietray Forages on Milk Fatty Acids Composition
Animal Husbandry and Feed Science    2009, 30 (10): 27-27.   DOI: 10.12160/j.issn.1672-5190.2009.10.011
Abstract101)      PDF(pc) (292KB)(189)       Save
Two key bioprocesses including ruminal bio-hydrogenation of fat and fatty acids synthesis in mammary, affect compositions of milk fatty acids, were analyzed, and fatty acids composition and characteristics of different forages were discussed in detail. Moreover, the effect of different dietary forages on milk fatty acids composition was particularly reviewed.
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Animal Husbandry and Feed Science    2010, 31 (1): 151-151.   DOI: 10.12160/j.issn.1672-5190.2010.01.079
Abstract176)      PDF(pc) (200KB)(258)       Save
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Animal Husbandry and Feed Science    2010, 31 (10): 120-120.   DOI: 10.12160/j.issn.1672-5190.2010.10.063
Abstract145)      PDF(pc) (194KB)(398)       Save
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Research Progress on the Colloidal Gold Immunochromatography Assay
Animal Husbandry and Feed Science    2013, 34 (12): 31-31.   DOI: 10.12160/j.issn.1672-5190.2013.12.011
Abstract132)      PDF(pc) (240KB)(341)       Save
Colloidal gold immunochromatography assay was a rapid immunological detection technology that was developed in 1980s after fluorescein, ELISA and radioisotope technology. Colloidal gold immunochromatography assay had been widely applied in medical examination, residual detection of veterinary drug and pesticide in agricultural products, pathogenic microorganism detection in import and export products and other fields because of its good stability, safety and convenient usage , none of instrument and equipment and other disadvantages. The development process, technical principle and application of colloidal gold immunochromatography assay were reviewed, so as to provide references for the related studies.
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Research Progress of bHLH Transcription Factors
WANG Li-wei, FANG Yong-yu, LIU Hong-kui, GUO Bao-min, WANG Yun-hua, SUN Rui-fen, JI Peng-hua, HE Jiang-feng
Animal Husbandry and Feed Science    2020, 41 (1): 23-27.   DOI: 10.12160/j.issn.1672-5190.2020.01.005
Abstract1741)      PDF(pc) (505KB)(752)       Save
Basic helix-loop-helix (bHLH) proteins which belong to the superfamily of transcription factors are widely distributed in eukaryotes. At present, most of the functions of bHLH transcription factors have been identified in animals, however, it's study in plants is relatively limited. As the second major transcription factors in plants, based on their interactions between specific amino acids and the target genes, bHLH transcription factors play important roles in plant growth and development, physiological metabolism and stress response, and then regulate the expression of associated genes. In order to further understand the information on function and characteristics of bHLH transcription factors in plants, we reviewed their structural features, classification and biological functions, with focus on the most recently reported investigations on their response to abiotic stress. Our review may provide a theoretical basis for further understanding the molecular mechanisms underlying the actions of plant bHLH transcription factors in response to stress.
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Animal Husbandry and Feed Science    2013, 34 (1): 123-123.   DOI: 10.12160/j.issn.1672-5190.2013.01.059
Abstract167)      PDF(pc) (171KB)(321)       Save
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Research Progress of Physiological Functions and Application of Hyaluronic Acid
Animal Husbandry and Feed Science    2015, 36 (1): 21-21.   DOI: 10.12160/j.issn.1672-5190.2015.01.008
Abstract361)      PDF(pc) (455KB)(457)       Save
Hyaluronic acid is a mucopolysaccharide widely distributed in the human body. While it has been successfully used in orthopedics,ophthalmology,plastic surgery, health food and other fields, there are still many new applications continue to be developed. The physiological function of hyaluronic acid were introduced and the research progress of its application in the above fields based on its structural characteristics are reviewed in this paper.
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Animal Husbandry and Feed Science    2009, 30 (7): 148-148.   DOI: 10.12160/j.issn.1672-5190.2009.07.040
Abstract174)      PDF(pc) (526KB)(302)       Save
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Animal Husbandry and Feed Science    2010, 31 (9): 156-156.   DOI: 10.12160/j.issn.1672-5190.2010.09.084
Abstract90)      PDF(pc) (312KB)(159)       Save
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Progress in Preparation and Application of Coenzyme Q10
Animal Husbandry and Feed Science    2010, 31 (9): 9-9.   DOI: 10.12160/j.issn.1672-5190.2010.09.005
Abstract389)      PDF(pc) (298KB)(292)       Save
Coenzyme Q10 is an important biologically active substance and widely present in organisms. It can enhance human immunity and antioxidant capacity and can also delay aging. At present, biosynthesis is a main preparation method and also future trend to produce coenzyme Q10 in industrial production. The eoenzyme Q10 has been applied in food, health products, cosmetics and other fields in addition to medicine field. The paper introduces progress in preparation and application of coenzyme Q10.
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Research Progress of Pharmaceutical Proanthocyanidin
Animal Husbandry and Feed Science    2011, 32 (2): 53-53.   DOI: 10.12160/j.issn.1672-5190.2011.02.026
Abstract178)      PDF(pc) (305KB)(246)       Save
The paper introduces recent progress in the applications of proanthocyanidin in prevention and treatment of cancer and angiocardiopathy as well as treatment of eye disease, and it also discusses the safety of proanthocyanidin in practice in order to provide a reference for further development of proanthocyanidin.
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Research Progress on the Extraction Technology of Alkaloids from Catharanthus roseus
Animal Husbandry and Feed Science    2012, 33 (3): 26-26.   DOI: 10.12160/j.issn.1672-5190.2012.03.010
Abstract129)      PDF(pc) (278KB)(265)       Save
Alkaloid is a kind of nitrogen-containing organic compounds that widely existed in the nature,which is of special medicinal value. The extraction, separation and content determination methods of alkaloid from natural medicinal herb Catharanthus roseus were summarized, so as to provide certain reference for the industrial production and scientific research of alkaloid in C. roseus.
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Animal Husbandry and Feed Science    2009, 30 (10): 80-80.   DOI: 10.12160/j.issn.1672-5190.2009.10.039
Abstract117)      PDF(pc) (293KB)(360)       Save
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Studies on the Nutritional Components of Fish Muscles and Human Health
Animal Husbandry and Feed Science    2013, 34 (5): 69-69.   DOI: 10.12160/j.issn.1672-5190.2013.05.023
Abstract282)      PDF(pc) (247KB)(355)       Save
The nutritional components of fish muscles had the characteristics of high protein, low fat content a variety of essential amino acids for human, unsaturated fatty acids and minerals. The effects of protein (amino acids), fat, minerals, vitamin and other nutritional elements in fish muscles on human health and functions were expounded. The research results showed that it was very important for human health to often eat fish.
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Animal Husbandry and Feed Science    2013, 34 (3): 113-113.   DOI: 10.12160/j.issn.1672-5190.2013.03.057
Abstract117)      PDF(pc) (249KB)(382)       Save
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Animal Husbandry and Feed Science    2008, 29 (4): 89-89.   DOI: 10.12160/j.issn.1672-5190.2008.04.046
Abstract189)      PDF(pc) (146KB)(191)       Save
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Animal Husbandry and Feed Science    2010, 31 (4): 101-101.   DOI: 10.12160/j.issn.1672-5190.2010.04.056
Abstract88)      PDF(pc) (190KB)(174)       Save
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Spatial-temporal Characteristics of Carbon Emissions from Livestock and Poultry Industry and Emission Reduction Paths: A Case Study of Henan Province
KONG Xiangfei,ZHANG Dongsheng
Animal Husbandry and Feed Science    2023, 44 (4): 61-69.   DOI: 10.12160/j.issn.1672-5190.2023.04.009
Abstract175)   HTML4)    PDF(pc) (893KB)(112)       Save

[Objective] This study analyzed the spatial-temporal characteristics of carbon emissions from livestock and poultry industry in Henan Province, proposed the countermeasures and suggestions on emission reduction paths, so as to provide references for accelerating the high-quality development of livestock and poultry industry in this province. [Method] The carbon emissions from livestock and poultry industry in Henan Province were taken as the research objects. Based on the total amount of cattle, horses, donkeys, mules, pigs, sheep and poultry for sale as well as the output value of livestock and poultry industry in Henan Province and its sub-administrative regions (17 prefecture-level cities and 1 county-level city directly under the province government) from the year of 2011 to 2020, and using CH4 and N2O produced by the intestine, stomach and excreta of various livestock and poultry as the source of greenhouse gases, the spatial-temporal characteristics of the total carbon emission equivalent and the carbon emission intensity of livestock and poultry industry in Henan Province and its sub-administrative regions were assessed by the carbon emission factor method and the carbon emission intensity calculation formula of livestock and poultry industry. [Result] In terms of the total carbon emission equivalent, the livestock and poultry industry and its various internal structures in Henan Province showed an overall downward trend from the year of 2011 to 2020. In terms of the year-on-year growth rate of the total carbon emission equivalent, the absolute carbon emission equivalent of livestock and poultry industry in Henan Province had gone through three stages with different fluctuation characteristics: a small increase stage, a significant decrease stage, and a fluctuation change stage. According to the characteristics of the absolute carbon emission equivalent of livestock and poultry industry, the sub-administrative regions of Henan Province could be categorized into five types: ′high-fluctuation′, ′medium-fluctuation′, ′medium-decline′, ′low-fluctuation′, and ′low-decline′. In terms of the absolute carbon emission intensity equivalent, the overall carbon emission intensity of livestock and poultry industry in Henan Province showed a downward trend from the year of 2011 to 2020, and the economic development quality of livestock and poultry industry improved greatly. In terms of the year-on-year growth rate of carbon emission intensity, the carbon emission intensity of livestock and poultry industry in Henan Province had undergone a fluctuating decline process, which was divided into two stages: a fluctuating decline stage and a fluctuating rise stage. According to the characteristics of the carbon emission intensity of livestock and poultry industry, the sub-administrative regions of Henan Province could be divided into 8 types: ′high-convergence growth′, ′high-fluctuation′, ′high-expansion decline′, ′medium-fluctuation′, ′medium-expansion decline′, ′medium-convergence decline′, ′low-fluctuation′, and ′low-convergence decline′. [Conclusion] Carbon emission reduction in livestock and poultry industry in Henan Province had made some progress in terms of quantity and quality. The obtained achievements in carbon emission reduction were unstable. The total carbon emission equivalent and the carbon emission intensity were characterized by regional heterogeneity. It is recommended that Henan Province achieve the modernized development of livestock and poultry industry through the following paths: strengthening the comprehensive utilization of livestock and poultry manures, enhancing the investment and application of science and technology in carbon emission reduction in livestock and poultry industry, optimizing the management practices of livestock and poultry, cooperatively promoting the greenhouse gas reduction through multiple policies, and accelerating the integrated development of the entire industry chain of livestock and poultry industry.

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Determination of Tryptophan in Feed by Microwave Hydrolysis and Amino Acid Automatic Analyzer
SONG Jie, WANG Li-fang, YAO Yi-ping, SHI Pei, ZHONG Hua-chen, GUO Chen-yang, ZHANG Xin-xin, HUANG Jie
Animal Husbandry and Feed Science    2021, 42 (2): 19-25.   DOI: 10.12160/j.issn.1672-5190.2021.02.004
Abstract320)      PDF(pc) (877KB)(237)       Save
[Objective] To develop a rapid determination method for tryptophan in feed by microwave hydrolysis and amino acid automatic analyzer. [Method] The optimum conditions of microwave alkaline hydrolysis were screened by single factor test and orthogonal test, and the tryptophan content in total mixed ration (TMR) of dairy cow was determined by the LCAK06/Na (4.6 mm×150 mm) column of automatic amino acid analyzer. [Result] Hydrolysis temperature was the most important factor affecting tryptophan content in feed. The optimum conditions for determination of tryptophan in feed by microwave alkaline hydrolysis were as follows: hydrolysis temperature 170 ℃, hydrolysis time 30 min, alkali concentration 5 mol/L. The tryptophan content in TMR was 0.14% determined by amino acid automatic analyzer. The linear range of tryptophan was 0.025-0.60 μmol/mL, and the correlation coefficient (r) was 0.999 7. The limit of quantification (LOQ) was 0.05%, the recovery rate of tryptophan was 93%-104%, and the RSD of precision was 3.58%. [Conclusion] The developed method is efficient and accurate, and there is no significant difference in detection results between this method and the conventional hydrolysis method in the national recommended standard of ′Determination of Tryptophan in Feed′ (GB/T 15400-2018), which has reference value for determination of tryptophan in feed.
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Research Status and Trend Analysis of Grassland Ecological Protection in China——Based on the Journal Literature Data from CNKI Database(1992-2021)
NING Ying, TIAN Yan-li
Animal Husbandry and Feed Science    2021, 42 (6): 68-75.   DOI: 10.12160/j.issn.1672-5190.2021.06.013
Abstract314)      PDF(pc) (1177KB)(171)       Save
[Objective] To analyze the state of grassland ecological protection research in China and investigate the most recent developments in order to provide suggestions and references for future study. [Method] Using the China National Knowledge Infrastructure (CNKI) database as the data retrieval source, CiteSpace software was employed to quantify the annual publication volume, author, organization, and keyword of 315 valid sample documents from the year of 1992 to 2021. [Result] Since the year of 2007, the number of annual publications in the field of grassland ecological protection in China has steadily increased, peaking in the year of 2009. Following then, the quantity of relevant documents reduced, but remained quite high. NING You-liang and HE You-jun are high-yield authors in the sector.The network of team cooperation has taken shape. LIU Tian-ming's team cooperates closely with other teams. There had been lots of cooperation among institutions, but only limited to certain regions and disciplines, deep cooperative relationships has not formed. At present, the research hotspots in the field of grassland ecological protection in China are primarily focused on grassland ecological protection subsidy and reward policy, grassland animal husbandry, grassland sustainable development and so on. [Conclusion] In China, grassland ecological protection research has yielded a wealth of information. Future study should focus more on grassland ecological restoration and management, as well as grassland ecological protection subsidy and reward policy.
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Animal Husbandry and Feed Science    2014, 35 (6): 121-121.   DOI: 10.12160/j.issn.1672-5190.2014.06.064
Abstract86)      PDF(pc) (273KB)(194)       Save
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Animal Husbandry and Feed Science    2013, 34 (7): 129-129.   DOI: 10.12160/j.issn.1672-5190.2013.07.068
Abstract120)      PDF(pc) (178KB)(296)       Save
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Animal Husbandry and Feed Science    2012, 33 (9): 95-95.   DOI: 10.12160/j.issn.1672-5190.2012.09.052
Abstract128)      PDF(pc) (179KB)(250)       Save
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Transcriptome Sequencing Analysis Reveals the Potential Molecular Mechanisms of Honeysuckle Extract in Protecting Newcastle Disease Virus Infection in Pigeon
MO Hongfang, SHI Zongcheng, YANG Yanyan, FENG Yun, YU Lintian, SHI Deshun, XIONG Xiaoyan
Animal Husbandry and Feed Science    2024, 45 (4): 10-19.   DOI: 10.12160/j.issn.1672-5190.2024.04.002
Abstract6127)      PDF(pc) (3568KB)(192)       Save
[Objective] This study was conducted to assess the mechanisms underlying the protective role of honeysuckle extract against Newcastle disease virus (NDV) infection in pigeon. [Method] A total of 40 healthy 28-day-old American king pigeons without NDV vaccination were selected and subjected to a 7-day adaptive rearing. At the age of 35 days, the experimental pigeons were randomly assigned into a honeysuckle extract protective (KO) group (n=10) or a virus challenge control (OE) group (n=10). The pigeons in KO group drank the water containing 1.2% honeysuckle extract for 7 consecutive days, and at the age of 42 days, they were intramuscularly injected with 0.5 mL NDV Pi/NJ/CH/4416/2016 strain of pigeon origin with HA potency of 5log2 . The pigeons in OE group drank the normal water and were challenged with the same NDV strain at the same dosage at the age of 42 days. Seven days after challenge, 3 pigeons were chosen from both KO and OE groups. The spleen samples were collected for paraffin-embedded tissue section preparation and for total RNA extraction to construct the transcriptome libraries. RNA-Seq was used to identify differentially expressed genes (DEGs) and to perform GO functional enrichment analysis as well as KEGG pathway enrichment analysis. Four randomly selected significant DEGs identified by RNA-Seq were verified by using qRT-PCR. [Result] The splenocytes of the pigeons in OE group had abnormally enlarged nuclei and white vacuoles, accompanied by a large number of granulocyte infiltration in splenic tissues, while those of the pigeons in KO group had many white vacuoles and minor granulocyte infiltration alone, and no abnormally enlarged splenocyte nuclei in splenic tissues were observed. A total of 374 DEGs were identified in the spleens of the pigeons in KO group by RNA-Seq, of which 112 were significantly up-regulated and 262 were significantly down-regulated. GO functional enrichment analysis demonstrated that 875 DEGs were enriched in 312 GO entries, of which 149 belonged to biological process, 33 belonged to cellular component, and 130 belonged to molecular function. KEGG pathway enrichment analysis revealed that 226 DEGs were enriched in 93 KEGG signaling pathways, including information transmission pathway, metabolic pathway, natural immunity related pathway, and apoptosis pathway. The qRT-PCR validation test showed that the relative mRNA abundance of the 4 randomly selected significant DEGs (LOC110360702, LOC110360688, SFRP5 and CHAD) had the same change trend as the RNA-Seq results. [Conclusion] Honeysuckle extract exerts protective role against NDV infection in pigeon by activating important physiological processes of host such as information transmission, metabolic regulation and immune response. The results obtained in this study provide a scientific basis for further investigation on the molecular mechanism of immune response to NDV infection in pigeon.
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Animal Husbandry and Feed Science    2008, 29 (2): 104-104.   DOI: 10.12160/j.issn.1672-5190.2008.02.048
Abstract137)      PDF(pc) (196KB)(211)       Save
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