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Isolation,Identification,Antimicrobial Resistance and Pathogenicity of a Staphylococcus aureus Strain from Feces of Sheep in Emin County,China
SU Zheng, YU Yuetong, ZHU Tingting, TUO Haixin, LI Jing, WU Jing, LIU Peng, PENG Jian, QI Meng
Animal Husbandry and Feed Science    2024, 45 (4): 103-108.   DOI: 10.12160/j.issn.1672-5190.2024.04.013
Abstract29)      PDF(pc) (2170KB)(49)       Save
[Objective] The aims of the present study were to isolate and identify the Staphylococcus aureusS. aureus) strain from fecal samples of lambs with diarrhoea, and to characterize the antimicrobial resistance and pathogenicity of the isolate. [Method] Four anal swab samples of diarrheic lambs were collected from a sheep farm in Emin County, Tacheng Prefecture, Xinjiang Uygur Autonomous Region, and S. aureus strain was selectively isolated with mannitol salt agar (MSA). The isolate was identified by morphological observation and 16S rDNA sequence analysis. The in vitro susceptibility of the isolate to 11 antimicrobial agents belonging to 9 categories was analyzed by paper diffusion method. The presence of virulence genes of the isolate was detected by PCR assay. A model of abdominal infection in Kunming mice was established to evaluate the pathogenicity of the isolate. [Result] One suspected S. aureus strain was isolated from the four fecal samples of diarrheic lambs. It formed glossy, moderately raised, golden or lemon coloured colonies on MSA. Gram staining and morphological observation revealed that the isolate was Gram-positive and arranged in the form of grape bunches. The 16S rDNA sequence of the isolate was 100% similar to that of a S. aureus isolate of porcineluny origin from Changsha City, Hunan Province, China. Morphological observation and 16S rDNA sequence analysis confirmed that the isolate was S. aureus. The isolate was resistant to penicillin, clindamycin, cefotiamine, rifampicin and erythromycin, and was sensitive to gentamicin, azithromycin, chloramphenicol, norfloxacin, cefoxitin and cotrimoxazole. Out of the 20 virulence genes belonging to 8 classes tested, the presence of sea, seg, sei, eta, clfA and clfB genes was observed. The LD50 of the isolate for Kunming mice was 2.24×108 CFU/mL, with observable pathological changes in the organs of the dead mice. [Conclusion] One S. aureus strain was isolated and identified from the fecal samples of diarrheic lambs in a sheep farm in Emin County, which exhibited a multi-drug resistant phenotype, harbored multiple virulence genes and had certain pathogenicity. The results of this study provide references for the rational use of medication for the diseases caused by S. aureus infection in sheep in this region.
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Research Progress of bHLH Transcription Factors
WANG Li-wei, FANG Yong-yu, LIU Hong-kui, GUO Bao-min, WANG Yun-hua, SUN Rui-fen, JI Peng-hua, HE Jiang-feng
Animal Husbandry and Feed Science    2020, 41 (1): 23-27.   DOI: 10.12160/j.issn.1672-5190.2020.01.005
Abstract1161)      PDF(pc) (505KB)(358)       Save
Basic helix-loop-helix (bHLH) proteins which belong to the superfamily of transcription factors are widely distributed in eukaryotes. At present, most of the functions of bHLH transcription factors have been identified in animals, however, it's study in plants is relatively limited. As the second major transcription factors in plants, based on their interactions between specific amino acids and the target genes, bHLH transcription factors play important roles in plant growth and development, physiological metabolism and stress response, and then regulate the expression of associated genes. In order to further understand the information on function and characteristics of bHLH transcription factors in plants, we reviewed their structural features, classification and biological functions, with focus on the most recently reported investigations on their response to abiotic stress. Our review may provide a theoretical basis for further understanding the molecular mechanisms underlying the actions of plant bHLH transcription factors in response to stress.
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Research Prospect in Application of Microecological Preparations in Animal Husbandry
PAN Yang-su, HE Yang, LI Yong-xiang, CAO Zhen-hui, HUANG Ying, AN Qing-cong, ZHANG Chun-yong, ZHAO Su-mei, HU Hong, PAN Hong-bin
Animal Husbandry and Feed Science    2023, 44 (1): 64-70.   DOI: 10.12160/j.issn.1672-5190.2023.01.010
Abstract171)   HTML4)    PDF(pc) (895KB)(114)       Save

As a novel-type of safe and green feed additives, microecological preparations have been widely used in animal husbandry production. The research progress on the application of microecological preparations in livestock and poultry farming industry is reviewed, and the application potential of Parabacteroides as the next generation of new probiotic additive in animal husbandry is expounded, in hoping to provide insights into the research and development of new microecological preparations for livestock and poultry.

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Study on the Chronic Toxicity of Melamine in Female Wistar Rats
Animal Husbandry and Feed Science    2012, 33 (4): 36-36.   DOI: 10.12160/j.issn.1672-5190.2012.04.014
Abstract84)      PDF(pc) (669KB)(52)       Save
Melamine(MA) is a low-acute toxicity chemical with LD50 of 4 550 mg/(kg·bw) in mice.To verify and evaluate its chronic toxicity,500,1 000 and 2 000 mg MA per kilogram feed were fed to 6-week-old female Wistar rats for six months.The results showed that functional disorders and organic changes of kidney,liver and ladder of female Wistar rats to different extent were observed in each test group.The blood biochemical indices(such as ALT,AST,CRE and UA) increased to different extent.The histological abnormalities or damages to different extent were found in kidney,liver and bladder.In 2 000 mg MA/kg dose group,the growth and feed intake were inhibited and histological change or damage of kidney was significant,even kidney calculi appeared.In poisoned group,no histological abnormality was found in spleen and bone marrow of rats.Maximal no-effect dose of MA on rats should be less than(35.7±15.4) mg/(kg·bw·d).
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Fast Detection Report of Melamine in Fresh Milk
Animal Husbandry and Feed Science    2013, 34 (10): 12-12.   DOI: 10.12160/j.issn.1672-5190.2013.10.008
Abstract256)      PDF(pc) (170KB)(55)       Save
[Objective] The research aimed to rapidly and accurately detect melamine in fresh milk and ensure the health and safety of fresh milk and its products. [ Method ] The content of melamine in 60 raw milk samples from milk cylinder and transport vehicles of 17 milk stations in Bazhou of Xinjiang was detected by enzyme linked immune method (SNAP). [Result] The detection results of melamine content in 60 fresh milk samples were negative. [ Conclusion ] The content of melamine in raw milk samples in milk cylinder and transport vehicles of 17 milk stations in Bazhou of Xinjiang and the results showed negative. 60 milk samples were detected and 60 samples were eligible, so the eligible rate reached 100%.
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