水稻藤仓镰孢菌对氰烯菌酯的抗药性分析

doi:10.12190/j.issn.2096-1197.2020.06.13

北方农业学报 ›› 2020, Vol. 48 ›› Issue (6): 81-86.doi: 10.12190/j.issn.2096-1197.2020.06.13

• 植物保护·农业生态环境 • 上一篇下一篇

水稻藤仓镰孢菌对氰烯菌酯的抗药性分析

张智慧, 金倩, 王夏雯, 王飞飞, 王信海

江苏省农业科学院宿迁农科所,江苏宿迁 223800

收稿日期:2020-10-31 出版日期:2020-12-20 发布日期:2021-02-04
通讯作者: 王信海(1982—),男,副研究员,硕士,主要从事生态综合种养的研究工作。
作者简介:张智慧(1992—),女,研究实习员,硕士,主要从事杀菌剂毒理与抗药性的研究工作。
基金资助:
宿迁市农业科技自主创新项目(SQCX201902)

Research on the resistance of Fusarium fujikuroi in rice to phenamacril

ZHANG Zhihui, JIN Qian, WANG Xiawen, WANG Feifei, WANG Xinhai

Suqian Institute of Agricultural Sciences,Jiangsu Academy of Agricultural Sciences,Suqian 223800,China

Received:2020-10-31 Online:2020-12-20 Published:2021-02-04

摘要/Abstract

摘要： 【目的】检测水稻藤仓镰孢菌对氰烯菌酯的抗药性,明确其抗药性机理。【方法】分别检测氰烯菌酯对采自江苏省宿迁地区的86株水稻恶苗病菌的最小抑制浓度(MIC),从中选取2株抗性菌株和2株敏感菌株,进行室内毒力、生长速率及产孢量测定,并对其myosin5基因进行序列分析。【结果】 68%藤仓镰孢菌对氰烯菌酯MIC>5 μg/mL。供试菌株Sq6、Sq12为抗药性菌株;Sq15、Sq20为敏感菌株,其菌丝生长速率不存在显著差异(P>0.05);Sq12和Sq20的产孢量显著高于Sq6和Sq15(P<0.05)。通过对myosin5基因序列比对分析,氰烯菌酯抗药性菌株(Sq6、Sq12)在326位密码子由精氨酸突变为丝氨酸。【结论】宿迁地区水稻藤仓镰孢菌对氰烯菌酯的抗药性频率为68%,在使用氰烯菌酯进行种子处理时,需考虑用药的频次和用量,减少或者延缓田间抗药性的产生。

关键词: 水稻, 藤仓镰孢菌, 氰烯菌酯, 抗药性, myosin5

Abstract: 【Objective】To determine the phenamicril resistance of Fusarium fujikuroi in rice and its resistance mechanisms.【Methods】The minimum inhibitory concentration(MIC)of phenamacril was tested from 86 Fusarium fujikuroi strains obtained from Suqian area of Jiangsu Province,two resistant strains and two sensitive strains were selected to test their virulence,growth rate and spore output in laboratory. The sequence of myosin5 gene was also analyzed.【Results】MIC of 68% Fusarium fujikuroi strains was >5 μg/mL to phenamicril,and strains Sq6 and Sq12 were resistant and Sq15 and Sq20 were sensitive,there was no significant difference in mycelial growth rate(P>0.05),and the spore output of Sq12 and Sq20 was significantly higher than that of Sq6 and Sq15(P<0.05). According to the sequence alignment of myosin5 gene,the resistant strains(Sq6,Sq12)mutated from arginine to serine at codon 326.【Conclusion】The resistance frequency of Fusarium fujikuroi in rice to phenamicril was 68% in Suqian. When phenamicril was used for seed dressing,the frequency and dosage should be considered in order to reduce or delay the development of resistance.

Key words: Rice, Fusarium fujikuroi, Phenamacril, Resistance, myosin5

中图分类号:

S511

张智慧, 金倩, 王夏雯, 王飞飞, 王信海. 水稻藤仓镰孢菌对氰烯菌酯的抗药性分析[J]. 北方农业学报, 2020, 48(6): 81-86.

ZHANG Zhihui, JIN Qian, WANG Xiawen, WANG Feifei, WANG Xinhai. Research on the resistance of Fusarium fujikuroi in rice to phenamacril[J]. Journal of Northern Agriculture, 2020, 48(6): 81-86.

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水稻藤仓镰孢菌对氰烯菌酯的抗药性分析

Research on the resistance of Fusarium fujikuroi in rice to phenamacril

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