北方农业学报 ›› 2022, Vol. 50 ›› Issue (6): 50-56.doi: 10.12190/j.issn.2096-1197.2022.06.07

• 土壤肥料·草业 • 上一篇    下一篇

微生物菌剂对连作蒙古黄芪生物量及土壤真菌群落结构的影响

栗瑞红1, 李红霞1, 李长胜1, 刘培京2, 王飞3, 顾扬4   

  1. 1.呼和浩特市农畜产品质量安全中心,内蒙古 呼和浩特 010020
    2.中国农业科学院 农业资源与农业区划研究所,北京 100081
    3.江西农业大学 生物科学与工程学院,江西 南昌 330045
    4.内蒙古盛齐堂生态药植有限公司,内蒙古 呼和浩特 011500
  • 收稿日期:2022-10-24 出版日期:2022-12-20 发布日期:2023-03-24
  • 通讯作者: 王飞
  • 作者简介:王 飞(1975—),男,副教授,博士,主要从事微生物资源与蛋白质工程的研究工作。
    栗瑞红(1974—),男,高级农艺师,学士,主要从事农产品质量安全与农业技术推广工作。
  • 基金资助:
    呼和浩特市科技计划项目(2019-社-5(2019-社-5)

Effects of microbial inoculants on biomass of continuous cropping Astragalus membranaceus var. mongholicus and soil fungal community structure

LI Ruihong1, LI Hongxia1, LI Changsheng1, LIU Peijing2, WANG Fei3, GU Yang4   

  1. 1. Hohhot Agricultural and Animal Products Quality and Safety Center,Hohhot 010020,China
    2. Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China
    3. College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang 330045,China
    4. Inner Mongolia Shengqitang Ecological Medicinal Plant Co. Ltd.,Hohhot 011500,China
  • Received:2022-10-24 Online:2022-12-20 Published:2023-03-24
  • Contact: WANG Fei

摘要:

【目的】明确不同微生物菌剂对连作蒙古黄芪生物量和土壤真菌群落结构的影响。【方法】以蒙古黄芪(Astragalus membranaceus var. mongholicus)为试验材料,设ETS复合菌剂(ETS)、多维特抗菌剂(DWTK)、多功能维生素菌剂(WSS)、空白对照(CK)4个处理,测定蒙古黄芪生物量,并采用高通量测序技术分析土壤真菌群落结构。【结果】DWTK处理对蒙古黄芪地上部和根部生物量有显著促进作用(P<0.05),其中,地上部生物量较CK提高17.5%、根部生物量较CK提高18.4%。3种微生物菌剂对土壤真菌区系的改善效果明显,其中,DWTK处理的土壤真菌OTU数量远高于CK和WSS处理,且真菌类群更加丰富;与其他3个处理相比,DWTK处理病原真菌Fusarium丰度显著降低,真菌Gibberella丰度显著提高。【结论】多维特抗菌剂可以有效改善蒙古黄芪根际真菌群落结构,减少病原真菌数量,提高真菌数量;有利于提高连作蒙古黄芪的抗病性,增加蒙古黄芪生物量。

关键词: 蒙古黄芪, 生物量, 微生物菌剂, 真菌群落结构

Abstract:

【Objective】To clarify the effects of different microbial inoculants on biomass of continuous cropping A. membranaceus var. mongholicus and soil fungal community structure.【Methods】 Four treatments were set up using A. membranaceus var. mongholicus as test material,ETS compound inoculant(ETS),Doveite antibacterial inoculant(DWTK),multifunctional vitamin bacterial inoculant(WSS)and blank control(CK). The biomass of A. membranaceus var. mongholicus was measured. And high-throughput sequencing technology was used to examine the fungal community structure in the soil.【Results】The biomass of A. membranaceus var. mongholicus above ground tissues and roots was significantly(P<0.05)increased by DWTK treatment,with above ground biomass increased by 17.5% and root biomass increased by 18.4%,compared with CK. The three microbial inoculants clearly improved the soil fungal community. Among them,the number of soil fungi OTU in DWTK treatment was substantially higher than that of CK and WSS treatment,and the fungal groups were more abundant. In comparison to the other three treatments,the DWTK treatment significantly reduced the abundance of pathogenic fungi Fusarium and increased the abundance of Gibberella.【Conclusion】Doveite antibacterial inoculant effectively improved the structure of rhizosphere fungi community of A. membranaceus var. mongholicus,reduced the number of pathogenic fungi,and increased the number of fungi. It was advantageous to strengthen the continuous cropping A. membranaceus var. mongholicus disease resistance and boost the biomass.

Key words: Astragalus membranaceus var. mongholicus, Biomass, Microbial inoculants, Fungi community structure

中图分类号: 

  • S567.239