关键词: 金莲花, 种子, 贮藏时间, 赤霉素浓度, 变温条件, 萌发活力

Abstract: 【Objective】To investigate the effects of storage time,gibberellin concentration,and varied temperature conditions on the seed germination activity of Trollius chinensis Bunge,and provide reference for the storage and germination activity conditions screening of seeds of T. chinensis Bunge.【Methods】Under different concentration gibberellin （100,250,500,800,1 000 mg/L）and varied temperature conditions（15/5,20/10,25/15,25/20,30/20,30/25 ℃）,dry storage for 1,2,and 3 years seeds of T. chinensis Bunge were used for germination test. And differential analysis of germination index was conducted on the seed of T. chinensis Bunge germination time of different treatments and seed germination index of different storage times（25/20 ℃,250 mg/L gibberellin）,different varied temperature conditions and storage times（250 mg/L gibberellin）,and different concentration gibberellin and different storage times（25/20 ℃）.【Results】The dry storage for 3 years seeds of T. chinensis Bunge germination beginning time and peak germination time were longer,but the shortest time of germination beginning time（8 d） occurred in the dry storage for 3,2,and 1 year,and the shortest time of peak germination time（10 d） only occurred in the dry storage for 1 year. Under the 25/20 ℃ varied temperature condition and 250 mg/L gibberellin treatment,the daily germination rate of the dry storage for 3 years seeds of T. chinensis Bunge were more lower than 2 and 1 year. Under the 250 mg/L gibberellin treatment,the germination rate and germination index of the dry storage for 2 and 1 years seeds of T. chinensis Bunge were significantly higher than 3 years at 25/20 ℃ varied temperature condition（P<0.05）;the germination rate of the dry storage for 3 years seeds of T. chinensis Bunge at 25/15 ℃ varied temperature conditions,for 2 years at 25/20 ℃ and for 1 year at 20/10 ℃ were the highest（20.87%,62.00%,and 74.73%）,respectively,the germination index of the dry storage for 3 years seeds of T. chinensis Bunge at 25/15 ℃ varied temperature conditions,for 2 and 1 years at 25/20 ℃ were the highest（1.26,5.20,and 5.38）,respectively. Under the 25/20 ℃ varied temperature condition,the germination rate and germination index of the dry storage for 2 and 1 year seeds of T. chinensis Bunge were significantly higher than 3 years（P<0.05） at treating with 250 mg/L gibberellin treatment,the germination rate and germination index of the dry storage for 3 years seeds treated with 100,250,500 mg/L gibberellin treatment,for 2 years treated with 250,500,1 000 mg/L gibberellin treatment and for 1 year treated with 500,800 mg/L gibberellin treatment were more higher.【Conclusion】Under the 25/20 ℃ varied temperature condition,with 250 mg/L and 500 mg/L gibberellin treatment is more conducive to improving the germination activity of dry storage for 3 and 2 years seeds of T. chinensis Bunge,and with 500 mg/L and 800 mg/L gibberellin treatment is more conducive to improving the germination activity of dry storage for 1 year seeds of T. chinensis Bunge.

Key words: Trollius chinensis Bunge, Seed, Storage time, Gibberellin concentration, Varied temperature conditions, Germination activity