Abstract: By research on the key prevention and co ntrol technology to bacterial canker of tomato, seed disinfection is the most crucial technology during prevention and control tomato ulcer disease, and robust seedling cultivation is important too.We researched the effect of the three different seedling culture modes with nutrition bowl, paper-tube and plug-seedling on disease incidence of bacterial canker of tomato. The result showed that the disease incidence of nutrition bowl seedling and paper-tube seedling in greenhouse and field is lower than plug-seedling if not hurt the root when we transplant seedlings,with stronger seedlings and developed root of tomato in nutrition bowl and paper-tube.