Abstract: With systemic investigation the temporal dynamic variation of population quantity of Trialeurodes vaporariorum in greenhouse in Wuhai area, it presented the trend of increased in all. The spatial distribution of Trialeurodes vaporariorum in greenhouse showed that the horizontal distribution of the order from high to low at north, middle, south, and with the increase of temperature, the Trialeurodes vaporariorum had the trend of migrating to the south of greenhouse; the vertical distribution of the sequence from high to low was upper, middle, lower, and the proportion of the total amount of the adult worm in the upper and middle leaves increased after the first decrease. Biological prevention and physical prevention can effectively restrain the population quantity of Trialeurodes vaporariorum in greenhouse.