Abstract: This paper m ainly focused on the tw o-dim ensional protein electrophoresis difference betw een various cytoplasmicmale sterility （CM S） about suger beets supplied by Inner Mongolia academ y of agricultural and anim al husbandry sciences.Seven kinds of suger beet had been selected through pollen research and m ark er-assisted selection.According to VNTRm icrosatellite m olecular m arker identification method the suger beets had the Owen infertility cytoplasm.Then through theflorescence analysis of reference group and treatm ent group between pollen situation and two -dim ensional proteinelectrophoresis, 23 difference protein spot had been found including 12 difference protein spot found in seedling stage ofreference group.After further analysis of the tw o-dim ensional protein electrophoresis of CMS between reference group andtreatm ent g roup,the difference protein spot num ber were 7 , 5 , 5 , 6 , 8 , 1 1 , 18.In the same time the 9 type with Owen infertilitycytoplasm and good pollen situation was analyzed and 5 difference protein spot had not been included in reference group.Prelim inary speculation was that nuclear genedid not control infertility cytoplasm gene protein expression but through proteinregulator control system lead to fertility restoring.