Abstract: NAC transcription factor family plays an important role in salt tolerance of plants. In this study, the NAC transcription factor family gene As NAC1 was cloned from oats, and the bioinformatics analysis was carried out. The results showed that the gene was 1 710 bp, encoding 320 amino acids, the isoelectric point was 6.62, and the molecular weight was 35.4 k D. The sequence analysis showed that As NAC1 was closely related to the KUP/HAK/KT of barley and wheat, and predicted that the protein had no hydrophobic transmembrane domain. Successfully constructed the plant p CAMBIA1301- As NAC1 expression vector, and transformed into Arabidopsis thaliana, obtained by comparison of salt stress under the condition of wild type Arabidopsis lines and transgenic lines phenotype, salt stress conditions, transgenic seed germination rate was 63%, and wild type was 43%; transgenic plant was wild type plants root length 1.39 times, 1.52 times and 1.37 times, fresh weight and dry weight of 1.50 times and 1.49 times,it indicated that salt tolerance of Arabidopsis plants were improved As NAC1 the expression.In order to provide experimental basis for revealing the salt tolerance mechanism of As NAC1 family gene NAC in plants, the study laid a foundation for improving the ability of plant salt tolerance by transgenic technology.