槲皮素对短星翅蝗生长发育及解毒酶活性的影响

doi:10.12190/j.issn.2096-1197.2021.02.11

摘要/Abstract

摘要： 【目的】初步明确槲皮素对短星翅蝗生长发育和主要解毒酶活性的影响,为防蝗生物农药的开发和利用提供依据。【方法】分别用0（CK）、0.001%、0.01%、0.1%和1%槲皮素溶液饲喂短星翅蝗3龄蝗蝻（雌虫）,测定蝗蝻7 d存活率、干重、生长速率和活性氧簇（ROS）水平,并检测细胞色素P450（CYP450）、谷胱甘肽-S-转移酶（GST）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性。【结果】与对照组相比,0.01%、0.1%和1%槲皮素处理短星翅蝗3龄蝗蝻后,蝗蝻7 d存活率分别下降18.29%、37.69%和53.68%,干重分别下降23.74%、28.25%和30.25%,生长速率分别下降58.02%、69.02%和73.96%;蝗蝻7 d存活率、干重和生长速率与槲皮素浓度显著（P<0.05）线性负相关。ROS水平分别上升95.66%、173.56%和157.04%,CYP450活性分别上升118.09%、142.61%和174.87%,GST活性分别上升143.83%、194.41%和235.62%,SOD活性分别上升75.47%、97.37%和107.95%,CAT活性分别上升299.49%、282.23%和333.74%;ROS水平、CYP450活性和GST活性与槲皮素浓度显著（P<0.05）线性正相关;SOD活性和CAT活性与槲皮素浓度呈线性正相关关系,但相关性不显著（P>0.05）。【结论】槲皮素对短星翅蝗具有一定抑制生长作用,在短星翅蝗绿色防控和生物农药开发方面具有潜在应用价值。

关键词: 槲皮素, 短星翅蝗, 存活率, 生长速率, 酶活性

Abstract: 【Objective】The effects of quercetin on the growth and development and main detoxification enzymes activities of Calliptamus abbreviatus were preliminarily determined,which provided the basis for the development and utilization of biological pesticides against Calliptamus abbreviatus.【Methods】The 3-year-old grasshoppers （female）were fed with 0（CK）,0.001%,0.01%,0.1% and 1% quercetin solution,respectively. It was measured that the 7-day survival rate,dry weight,growth rate,reactive oxygen species（ROS）levels,and the activities of cytochrome P450（CYP450）,glutathione S-transferase（GST）,superoxide dismutase（SOD）and catalase（CAT）.【Results】Compared with CK,after treated with 0.01%,0.1% and 1% quercetin,the 7-day survival rate decreased by 18.29%,37.69% and 53.68%,dry weight decreased by 23.74%,28.25% and 30.25%,and growth rate decreased by 58.02%,69.02% and 73.96%,respectively. The 7-day survival rate,dry weight and growth rate were significantly negatively correlated with quercetin concentration（P<0.05）. ROS levels increased by 95.66%,173.56% and 157.04%,the activities of CYP450 increased by 118.09%,142.61% and 174.87%,the activities of GST increased by 143.83%,194.41% and 235.62%,the activities of SOD increased by 75.47%,97.37% and 107.95%,the activities of CAT increased by 299.49%,282.23% and 333.74%. ROS levels,the activities of CYP450 and the activities of GST were significantly positively correlated with quercetin concentration（P<0.05）. The activities of SOD and the activities of CAT were showing positively correlated with quercetin concentration,but the correlation had no significant difference（P>0.05）.【Conclusion】Quercetin has a certain inhibitory effect on the growth of Calliptamus abbreviatus,and has potential application value in the biological pesticide for the green control of Calliptamus abbreviates.

Key words: Quercetin, Calliptamus abbreviatus, Survival rate, Growth rate, Enzymatic activity

中图分类号:

S433.2

黄训兵, 王馨超, 李辉. 槲皮素对短星翅蝗生长发育及解毒酶活性的影响[J]. 北方农业学报, 2021, 49(2): 71-77.

HUANG Xunbing, WANG Xinchao, LI Hui. Effects of quercetin on the growth and development and detoxification enzyme activities of Calliptamus abbreviatus[J]. Journal of Northern Agriculture, 2021, 49(2): 71-77.

参考文献

[1] RODA A L,BALDWIN I T.Molecular technology reveals how the induced direct defenses of plants work[J].Basic & Applied Ecology,2003,4(1):15-26.
[2] WU J Q,BALDWIN I T.New insights into plant responses to the attack from insect herbivores[J].Annual Review of Genetics,2010,44:1-24.
[3] MICHAEL W.Plant secondary metabolites modulate insect behavior-steps toward addiction?[J].Frontiers in Physiology,2018,9:364.DOI:10.3389/fphys.2018.00364.
[4] 朱俊洪,程立生.植物次生性物质与植物抗虫性的关系及其在害虫防治中的应用前景[J].华南热带农业大学学报,2001,7(1):26-32.
[5] DESPRÉS L,DAVID J P,GALLET C.The evolutionary ecology of insect resistance to plant chemicals[J].Trends in Ecology & Evolution,2007,22(6):298-307.
[6] ROY A,WALKER W B,VOGEL H,et al.Diet dependent metabolic responses in three generalist insect herbivores Spodoptera spp.[J].Insect Biochemistry & Molecular Biology,2016,71:91-105.
[7] SIMMONDS M S J.Flavonoid-insect interactions:recent advances in our knowledge[J].Phytochemistry,2003,64(1):21-30.
[8] POREDDY S,MITRA S,SCHÖTTNER M,et al.Detoxification of hostplant′s chemical defence rather than its anti-predator co-option drives β-glucosidase-mediated lepidopteran counteradaptation[J].Nature Communications,2015,6(1):164-174.
[9] ZÜST T,AGRAWAL A A.Trade-offs between plant growth and defense against insect herbivory:An emerging mechanistic synthesis[J].Annual Review of Plant Biology,2017,68(1):513-534.
[10] JALLOW M F A,AWADH D G,ALBAHO M S,et al.Pesticide knowledge and safety practices among farm workers in Kuwait:results of a survey[J/OL].International Journal of Environmental Research & Public Health,2017,14(4):e340[2017-03-29].http://www.doc88.com/p-5465646957822.html.DOI:10.3390/ijerph14040340.
[11] SENTHIL-NATHAN S.Physiological and biochemical effect of neem and other Meliaceae plants secondary metabolites against Lepidopteran insects[J/OL].Frontiers in Physiology,2013,4:359[2015-11-02].http://www.doc88.com/p-9973413658564.html.DOI:10.3389/fphys.2013.00359.
[12] PRASAD A K,MUKHOPADHYAY A.Growth,nutritional indices and digestive enzymes of Hyposidra infixaria Walker(Lepidoptera:Geometridae)on artificial and natural(tea)diets[J].Journal of Asia-Pacific Entomology,2016,19(1):167-172.
[13] SCHULER M A,BERENBAUM M R.Structure and function of cytochrome P450s in insect adaptation to natural and synthetic toxins:insights gained from molecular modeling[J].Journal of Chemical Ecology,2013,39(9):1232-1245.
[14] WANG R L,LIU S W,BAERSON S R,et al.Identification and functional analysis of a novel cytochrome P450 gene CYP9A105 associated with pyrethroid detoxification in Spodoptera exigua Hübner[J].International Journal of Molecular Sciences,2018,19(3):737.DOI:10.3390/ijms19030737.
[15] CHAN B G,WAISS A C J,BINDER R G,et al.Inhibition of lepidopterous larval growth by cotton constituents[J].Entomologia Experimentalis et Applicata,1978,24(3):294-300.
[16] ABOU-ZAID M M,BENINGER C W,ARNASON J T,et al.The effect of one flavone,two catechins and four flavonols on mortality and growth of the European corn borer(Ostrinia nubilalis Hubner)[J].Biochemical Systematics & Ecology,1993,21(4):415-420.
[17] 张月娥. 槲皮素和芦丁在家蚕幼虫中对解毒酶的诱导模式[D].重庆:重庆大学,2012.
[18] 卢辉,韩建国.典型草原三种蝗虫种群死亡率和竞争的研究[J].草地学报,2008,16(5):480-484.
[19] 张靓,宋丽梅,王广君,等.蝗虫微孢子虫和绿僵菌不同配方饵剂对短星翅蝗和宽须蚁蝗的室内生测研究[J].草地学报,2016,24(1):171-177.
[20] 田方文. 紫花苜蓿田短星翅蝗发生规律及防治对策[J].草业科学,2003,20(12):80-81.
[21] 赵铂锤,纪明山,李修伟,等.草原短星翅蝗防治药剂的室内筛选[J].湖南农业科学,2013(23):76-80.
[22] ANDERSON T D,ZHU K Y.Synergistic and antagonistic effects of atrazine on the toxicity of organophosphorodithioate and organophosphorothioate insecticides to Chironomus tentans(Diptera:Chironomidae)[J].Pesticide Biochemistry and Physiology,2004,80(1):54-64.
[23] ZHU K Y,GAO J R,STARKEY S R.Organophosphate resistance mediated by alterations of acetylcholinesterase in a resistant clone of the greenbug,Schizaphis graminum(Homoptera:Aphididae)[J].Pesticide Biochemistry & Physiology,2000,68(3):138-147.
[24] BEAUCHAMP C,FRIDOVICH I.Superoxide dismutase:Improved assays and an assay applicable to acrylamide gels[J].Analytical Biochemistry,1971,44(1):276-287.
[25] MAEHLY A C,CHANCE B.The assay of catalases and peroxidases.[J].Methods of Biochemical Analysis,1954,1:357-424.
[26] 邓鸿飞,桑晓清,周利娟.植物源次生代谢物质的杀虫作用机制[J].世界农药,2011,33(3):17-21.
[27] KHAN S,TANING C N T,BONNEURE E,et al.Insecticidal activity of plant-derived extracts against different economically important pest insects[J].Phytoparasitica,2017,45(1):113-124.
[28] 张永亮,朱勇.槲皮素和硫脲对野桑蚕多酚氧化酶的抑制类型[J].湖北农业科学,2011,50(21):4399-4400.
[29] 王瑞龙,孙玉林,梁笑婷,等.6种植物次生物质对斜纹夜蛾解毒酶活性的影响[J].生态学报,2012,32(16):5191-5198.
[30] MATSUMURA T,MATSUMOTO H,HAYAKAWA Y.Heat stress hardening of oriental armyworms is induced by a transient elevation of reactive oxygen species during sublethal stress[J].Archives of Insect Biochemistry and Physiology,2017,96(3):e21421.DOI:10.1002/arch.21421.
[31] HUANG J F,LV C J,HU M Y,et al.The mitochondria-mediate apoptosis of lepidopteran cells induced by azadirachtin[J].PLoS One,2013,8(3):e58499.DOI:10.1371/journal.pone.0058499.
[32] AUCOIN R R,PHILOGÈNE B J R,ARNASON J T.Antioxidant enzymes as biochemical defenses against phototoxin-induced oxidative stress in three species of herbivorous lepidoptera[J].Archives of Insect Biochemistry and Physiology,1991,16(2):139-152.
[33] LEE K,BERENBAUM M R.Food utilization and antioxidant enzyme activities of black swallowtail in response to plant phototoxins[J].Archives of Insect Biochemistry and Physiology,1993,23(2):79-89.
[34] MITTAPALLI O,NEAL J J,SHUKLE R H.Antioxidant defense response in a galling insect[J].Proceedings of the National Academy of Sciences,2007,104(6):1889-1894.
[35] BIRNBAUM S S L,RINKER D C,GERARDO N M,et al.Transcriptional profile and differential fitness in a specialist milkweed insect across host plants varying in toxicity[J].Molecular Ecology,2017,26(23):6742-6761.
[36] WHITMAN D W,ANANTHRAKRISHNAN T N.Phenotypic plasticity of insects:Mechanisms and consequences[J].The Quarterly Review of Biology,2009,84(4):422-423.