生物菌肥对马铃薯4种土传病害病原菌的室内抑制效果

doi:10.12190/j.issn.2096-1197.2021.02.12

北方农业学报 ›› 2021, Vol. 49 ›› Issue (2): 78-83.doi: 10.12190/j.issn.2096-1197.2021.02.12

• 农业生态环境·植物保护 • 上一篇下一篇

生物菌肥对马铃薯4种土传病害病原菌的室内抑制效果

赵远征¹, 王东², 张翔宇³, 聂峰杰⁴, 彭敏⁵, 姜金涛⁶, 徐利敏¹

1.内蒙古自治区农牧业科学院植物保护研究所,内蒙古呼和浩特 010031;
2.内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特 010011;
3.乌兰察布市植保植检站,内蒙古集宁 012000;
4.宁夏农林科学院农业生物技术研究中心,宁夏银川 750002;
5.元泰丰（包头）生物科技有限公司,内蒙古萨拉齐 014100;
6.内蒙古智诚物联股份有限公司,内蒙古集宁 012000

收稿日期:2020-10-16 出版日期:2021-04-20 发布日期:2021-06-21
通讯作者: 徐利敏（1964—）,男,研究员,学士,主要从事马铃薯病虫草害综合防控技术的研究工作。
作者简介:赵远征（1986—）,女,助理研究员,博士,主要从事作物病虫草害及生物互作的研究工作。
基金资助:
内蒙古农牧业创新基金项目（2019CXJJNO7）; 内蒙古自治区科技计划项目（2019GG180）; 内蒙古自治区科技成果转化专项（2019CG026）

Indoor inhibitory effect of microbial fertilizer on four soil-borne diseases pathogens of potato

ZHAO Yuanzheng¹, WANG Dong², ZHANG Xiangyu³, NIE Fengjie⁴, PENG Min⁵, JIANG Jintao⁶, XU Limin¹

1. Institute of Plant Protection,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
2. College of Horticulture and Plant Protection,Inner Mongolia Agricultrual University,Hohhot 010011,China;
3. Ulanqab Plant Protection and Inspection Station,Jining 012000,China;
4. Centre of Agricultural Biotechnology,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,China;
5. Yuan Tai Fung（Baotou）Biotechnology Co., Ltd., Salaqi 014100,China;
6. Inner Mongolia Zhicheng IOT Co., Ltd., Jining 012000,China

Received:2020-10-16 Online:2021-04-20 Published:2021-06-21

摘要/Abstract

摘要： 【目的】明确供试生物菌肥对马铃薯4种土传病害病原菌（加利利链霉菌、大丽轮枝菌、尖孢镰刀菌和立枯丝核菌）的室内抑制效果。【方法】通过菌丝生长速率法,对不同浓度生物菌肥（50、100、1 000 mg/L）处理下的马铃薯4种土传病害病原菌进行抑制效果测定,并测定了生物菌肥对4种病原菌的毒力。【结果】生物菌肥对同种病原菌的抑制率会随着浓度的增加而增大;在浓度1 000 mg/L时对4种病原菌的抑制效果最佳,对加利利链霉菌和大丽轮枝菌的抑制率分别为97.44%和85.01%,对尖孢镰刀菌和立枯丝核菌的抑制率分别为76.62%和32.07%。生物菌肥对4种病原菌的毒力作用存在一定差异,对大丽轮枝菌和加利利链霉菌的EC₅₀值较小,分别为18.251 mg/L和41.461 mg/L;对尖孢镰刀菌的EC₅₀值为140.939 mg/L;对立枯丝核菌的EC₅₀值最大,为18 632.649 mg/L。【结论】供试生物菌肥对大丽轮枝菌和加利利链霉菌具有较强的抑制效果和毒力,对尖孢镰刀菌具有一定的抑制效果和毒力,但对立枯丝核菌的抑制效果和毒力较小。

关键词: 马铃薯, 病原菌, 生物菌肥, 抑制率, 毒力

Abstract: 【Objective】To determine the indoor inhibitory effect of the tested microbial fertilizers on the four soil-borne disease pathogens of potato（Streptomyces galilaeus,Verticillium dahliae,Fusarium oxysporum and Rhizoctonia solani）.【Methods】By the method of mycelial growth rate,the inhibitory effect of four soil-borne disease pathogens of potato treated with different concentrations of microbial fertilizer（50,100,1 000 mg/L）was determined,and the effect of microbial fertilizer on the 4 pathogens toxicity was determined.【Results】The inhibition ratio of same pathogen was improved with the increase of concentration of microbial fertilizer;the greatest inhibitory effect showed when four pathogens treated with 1 000 mg/L microbial fertilizer,and the inhibition ratio of Streptomyces galilaeus and Verticillium dahliae were 97.44% and 85.01%,the inhibition ratio of Fusarium oxysporum and Rhizoctonia solani were 76.62% and 32.07%. The microbial fertilizer showed different toxicity effects on 4 pathogens,EC₅₀ of Verticillium dahliae and Streptomyces galilaeus were smaller and were 18.251 mg/L and 41.461 mg/L;EC₅₀ of Fusarium oxysporum was 140.939 mg/L,while EC₅₀ of Rhizoctonia solani was the biggest（18 632.649 mg/L）.【Conclusion】The tested microbial fertilizer had a strong inhibitory effect and toxicity against Verticillium dahliae and Streptomyces galilaeus,and has a certain inhibitory effect and toxicity against Fusarium oxysporum,but it has a lesser inhibitory effect and toxicity against Rhizoctonia solani.

Key words: Potato, Pathogen, Microbial fertilizer, Inhibition ratio, Toxicity

中图分类号:

S532

赵远征, 王东, 张翔宇, 聂峰杰, 彭敏, 姜金涛, 徐利敏. 生物菌肥对马铃薯4种土传病害病原菌的室内抑制效果[J]. 北方农业学报, 2021, 49(2): 78-83.

ZHAO Yuanzheng, WANG Dong, ZHANG Xiangyu, NIE Fengjie, PENG Min, JIANG Jintao, XU Limin. Indoor inhibitory effect of microbial fertilizer on four soil-borne diseases pathogens of potato[J]. Journal of Northern Agriculture, 2021, 49(2): 78-83.

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生物菌肥对马铃薯4种土传病害病原菌的室内抑制效果

Indoor inhibitory effect of microbial fertilizer on four soil-borne diseases pathogens of potato

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