油菜水浸提液自毒作用的研究

doi:10.12190/j.issn.2096-1197.2023.01.08

摘要/Abstract

摘要： 【目的】探讨油菜水浸提液对自身幼苗生长的影响。【方法】室内提取油菜水浸提液,以蒸馏水为对照,设置3个浓度（0.025、0.050、0.100 g/mL）分析其对油菜种子发芽、幼苗生长﹑主要营养物质和防御酶活性的影响。【结果】与对照相比,油菜水浸提液0.050 g/mL和0.100 g/mL可显著降低油菜种子发芽率（P<0.05）;0.025 g/mL浓度时油菜茎长表现为促进作用,0.050、0.100 g/mL浓度时油菜茎长表现为抑制作用;3个浓度均对油菜幼苗根长产生抑制作用。随着油菜水浸提液浓度升高,油菜化感综合效应表现为“低促高抑”。与对照相比,0.100 g/mL浓度可显著降低油菜蛋白质含量（P<0.05）;0.050 g/mL浓度时油菜的脯氨酸含量较对照高115.39%;经不同浓度水浸提液处理后油菜的SOD活性均显著低于对照（P<0.05）;在水浸提液浓度为0.025 g/mL时油菜幼苗POD活性显著高于对照（P<0.05）;不同浓度水浸提液处理后的油菜丙二醛含量变化不同。【结论】油菜具有较强的自毒作用。

关键词: 油菜, 水浸提液, 自毒作用

Abstract: 【Objective】To explore the effect of canola water extract on the growth of the seedlings.【Methods】Using distilled water as the control,three concentrations（0.025,0.050,and 0.100 g/mL）of laboratory prepared canola water extract were set to analyze the effects on canola seed germination,seedling growth,main nutrients,and defense enzyme activity.【Results】Compared with the control,the canola water extract of 0.050 g/mL and 0.100 g/mL significantly（P<0.05）reduced the seed germination rate of canola. At the concentration of 0.025 g/mL there were promotion effects on stem length,while at concentrations of 0.050 and 0.100 g/mL,there were inhibitory effects on stem length. All three concentrations had inhibitory effects on the root length of canola seedlings. As the concentration of canola water extract increased,the allelopathic comprehensive effects of canola were "low promotion and high inhibition". In comparison with the control,concentration of 0.100 g/mL significantly（P<0.05）reduced the canola protein content. At concentration of 0.050 g/mL canola proline content was 115.39% higher than the control. The SOD activity of canola treated with different concentrations of water extract was significantly（P<0.05）lower than the control. At the concentration of 0.025 g/mL,the POD activity of canola seedlings was significantly（P<0.05）higher than the control. Different concentrations of water extract treatment had different effect on the variations in canola malondialdehyde content.【Conclusion】Strong autotoxicity existed in canola.

Key words: Canola, Water extract, Autotoxicity

中图分类号:

S634.3

石丹妮, 冯煜琳, 常静, 李睿光, 高玉莲, 李海平. 油菜水浸提液自毒作用的研究[J]. 北方农业学报, 2023, 51(1): 72-78.

SHI Danni, FENG Yulin, CHANG Jing, LI Ruiguang, GAO Yulian, LI Haiping. Study on autotoxicity of canola water extract[J]. Journal of Northern Agriculture, 2023, 51(1): 72-78.

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