基因枪介导抗草甘膦基因对玉米自交系A188的遗传转化

doi:10.12190/j.issn.2096-1197.2020.06.01

Abstract

Abstract: 【Objective】In order to effectively use herbicides for weed control,the herbicide resistant transgenic maize germplasm materials were cultivated,and the transgenic maize breeding technology was further improved.【Methods】Genetic transformation was conducted on immature embryos of callus induction of maize inbred line A188 by particle bombardment,which was carried out single factor test,the different size of immature embryo and concentration of herbicide were set respectively,and the callus induction rate was compared and analyzed.【Results】The results showed that the callus induction rate was the highest(93.3%)when the size of the immature embryo was 1.5-2.0 mm. When the concentration of bialaphos was 3 mg/L,it was the best screening concentration of herbicide. PCR detection proved that 5 positive transgenic seedlings were successfully obtained,and they showed strong glyphosate resistance in transgenic seedlings of resistance identification tests.【Conclusion】This study provides a reference for the establishment of maize genetic transformation system mediated by particle bombardment and for the cultivation of herbicide-resistant maize germplasm materials.

Key words: Maize, Glyphosate-resistance gene(EPSPS), Particle bombardment, Genetic transformation

CLC Number:

S513

HAN Ping‘an, WU Xinrong, SUN Ruifen, CHANG Yue, SHI Haibo, TANG Kuangang, LIANG Yahui, LI Xiaodong. Genetic transformation of maize inbred line A188 with the glyphosate-resistant genemediated by particle bombardment[J].Journal of Northern Agriculture, 2020, 48(6): 1-5.

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Genetic transformation of maize inbred line A188 with the glyphosate-resistant genemediated by particle bombardment

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