设施番茄灰霉病病原菌的分离与鉴定

doi:10.12190/j.issn.2096-1197.2023.02.10

Abstract

Abstract: 【Objective】To identify the pathogen species causing gray mold on facility tomato.【Methods】Suspected gray mold disease samples were isolated from tomato cultivation plots in three facilities in Hohhot City and Ulanqab City,Inner Mongolia. Morphological and molecular biology methods were used to identify the isolated strains. The pathogenicity of the pathogens was determined and re-isolated on detached stems and fruits according to Koch′s rule.【Results】Seven strains of pathogen were isolated from diseased stems and fruits. The ITS rDNA sequences were 99.60%-100% homologous with Botrytis cinerea（GenBank registration number KP151610,KX426964,and ON566787）,indicating that all the seven strains were Botrytis cinerea. Two strains,G4 and JW1,were selected and inoculated on the stems and fruits of healthy facility tomato. The incidence rate of gray mold was 100%.【Conclusion】The gray mold pathogen in facility tomato was Botrytis cinerea.

Key words: Tomato, Gray mold, Botrytis cinerea

CLC Number:

S436.412.13

GAO Jing, LIU Yan, YANG Yongqing, DI Jiezeng, WANG Yong, KANG Liru, XIAO Meng. Isolation and identification of gray mold pathogen on facility tomato[J].Journal of Northern Agriculture, 2023, 51(2): 83-90.

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Isolation and identification of gray mold pathogen on facility tomato

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