毛叶苕子与燕麦混播对作物产量、土壤酶活性和微生物群落的影响

doi:10.12190/j.issn.2096-1197.2024.03.10

Abstract

Abstract: 【Objective】To investigate the effects of different mixed sowing proportions of hairy vetch and oat on crop yield,soil enzyme activity and microbial communities in the agricultural dry-crop areas at the northern foothills of the Yinshan Mountains in Inner Mongolia.【Methods】Using hairy vetch and oat as experimental objects,six treatments were set up,namely,single sowing of hairy vetch（DB1）,single sowing of oat（DB2）,80% hairy vetch+ 20% oat（HB1）,60% hairy vetch+40% oat（HB2）,40% hairy vetch+60% oat（HB3）,and 20% hairy vetch+80% oat（HB3）. Soil samples were collected from the 0-20 and 20-40 cm soil layers in the early,middle,and late stages of growth,respectively. The effects on crop yield,soil enzyme activity and microbial biomass carbon and nitrogen content were analyzed,and changes in soil microbial community diversity and structure were analyzed by using high-throughput sequencing technology.【Results】HB1,HB2,HB3 treatments were 1%-11% higher than DB1 treatment for the relative grain yield of hairy vetch,and 3%-25% higher than DB2 treatment for the relative grain yield of oat. During the mid growth stage in the 0-20 cm soil layer,compared with DB1 and DB2 treatments,HB2 treatment soil catalase activity significantly increased by 9.65% and 11.55% （P<0.05）,respectively,and sucrase activity significantly increased by 11.47% and 15.44%（P<0.05）,HB1 treatment increased urease activity by 8.07% and 19.41%（P<0.05）,respectively;HB2 treatment had the highest microbial biomass carbon content,while HB1 treatment had the highest microbial biomass nitrogen content,at 95.79 and 21.02 mg/kg,respectively. Compared with the DB2 treatment,the HB2 treatment soil bacterial OTU index was significantly increased by 15.79%（P<0.05）,the Shannon index increased by 5.41%;Compared with DB1 treatment,HB2 treatment increased fungal OTU index by 33.58%（P<0.05）,Shannon index increased by 36.50%,and Chao1 index increased by 6.56%. At the phylum level,HB2 treatment increased the relative abundance of Proteobacteria,Chloroflexi,and Mortierellomycota,while decreasing the relative abundance of Actinobacteriota,Acidobacteriota,Ascomycota,and Basidiomycota.【Conclusion】The mixed sowing treatment of 60% hairy vetch +40% oat is a suitable proportion for agricultural dry-crop areas in the northern foothills of Yinshan Mountains,Inner Mongolia. Under this treatment,crop grain yield is the highest,soil enzyme activity and microbial biomass carbon and nitrogen content are higher,and soil bacterial and fungal diversity,richness,and relative abundance of beneficial bacterial phyla are all increased.

Key words: Hairy vetch, Oat, Mixed sowing, Crop yield, Soil enzyme activity, Microbial community

CLC Number:

S551.2

WANG Xuanming, YANG Qinzhong, ZHANG Peng, ZHAO Peiyi, REN Yongfeng, HAN Yunfei, DU Erxiao, ZHANG Biao, LI Shusheng. The effects of mixed sowing of hairy vetch and oat on crop yield,soil enzyme activity and microbial communities[J].Journal of Northern Agriculture, 2024, 52(3): 88-99.

References

[1] 贺启珅,陈大岭,李邵宇,等.阴山北麓不同恢复年限弃耕地植物群落生物量与土壤养分变化[J].中国草地学报,2022,44(10):30-37.
[2] 来幸梁,师尚礼,白杉杉,等.紫花苜蓿与禾本科牧草混播对土壤酶活性的影响[J].草原与草坪,2021,41(2):75-83.
[3] 于铁峰,郝凤,张永亮,等.沙地豆禾混播草地土壤酶与土壤养分对混播比例的响应[J].草地学报,2021,29(6):1217-1223.
[4] 齐都吉雅. 种植方式对苜蓿人工草地土壤微生物量碳、氮的影响[D].呼和浩特:内蒙古农业大学,2013.
[5] CHINTA Y D,UCHIDA Y,ARAKI H.Improvements of soil biological properties with hairy vetch(Vicia villosa Roth) andrye(Secale cereale L.) andthe contributions to lettuce(Lactuca sativa L.) production[J].Acta Horticulturae,2020,1286(1286):PP111-118.
[6] 赵艳兰. 豆禾混播组合与比例对草地生产力及土壤微生物群落的影响[D].雅安:四川农业大学,2023.
[7] 肖毅. 苜蓿/鸭茅不同行比混播模式增产增效机制研究[D].太原:山西农业大学,2021.
[8] CARPICI E B,TUNALI M M.Effects of the nitrogen and phosphorus fertilization on the yield and quality of the hairy vetch Vicia villosa Roth and barley Hordeum vulgare L. mixture[J].African Journal of Biotechnology,2012,11(28):7208-7211.
[9] SANDHYA RANI Y,JAMUNA P,TRIVENI U,et al.Effect of in situ incorporation of legume green manure crops on nutrient bioavailability,productivity and uptake of maize[J].Journal of Plant Nutrition,2022,45(7):1004-1016.
[10] 张君,段玉,刘亚红,等.不同施肥处理对燕麦产量、养分吸收和水肥利用效率的影响[J].北方农业学报,2020,48(5):62-68.
[11] 周礼恺,张志明,周礼恺,等.土壤酶活性的测定方法[J].土壤通报,1980(5):37-38.
[12] 关松荫. 土壤酶及其研究法[M].北京:农业出版社,1986.
[13] 丰骁,段建平,蒲小鹏,等.土壤脲酶活性两种测定方法的比较[J].草原与草坪,2008(2):70-72.
[14] 林启美,吴玉光,刘焕龙.熏蒸法测定土壤微生物量碳的改进[J].生态学杂志,1999(2):64-67.
[15] 黄懿梅,安韶山,曲东,等.两种测定土壤微生物量氮方法的比较初探[J].植物营养与肥料学报,2005(6):124-129.
[16] 郭童鑫,姚有华,吴昆仑,等.基于种间关系和生产性能评价的青稞豌豆混作模式研究[J].西北农业学报,2022,31(10):1287-1301.
[17] 冯琴,王斌,王腾飞,等.不同播种量毛苕子与燕麦混播对草地生产性能及营养品质的影响[J].草地学报,2022,30(12):3439-3446.
[18] 谭海运. 西藏高寒干旱半干旱地区饲草混播试验研究[J].西藏农业科技,2022,44(1):47-49.
[19] 谢开云,赵云,李向林,等.豆-禾混播草地种间关系研究进展[J].草业学报,2013,22(3):284-296.
[20] 邵春慧,徐强,史志强,等.夏河农牧交错区小黑麦与豆科牧草混播的生产性能研究[J].草地学报,2022,30(10):2791-2801.
[21] 李满有,杨彦军,王斌,等.干旱地区滴灌条件下燕麦与箭筈豌豆混播模式研究[J].西北农林科技大学学报(自然科学版),2021,49(12):51-57.
[22] 高彦波,翟鹏辉,谭德远.土壤酶活性与土壤肥力的关系研究进展[J].安徽农业科学,2015,43(6):100-101.
[23] 王理德,王方琳,郭春秀,等.土壤酶学研究进展[J].土壤,2016,48(1):12-21.
[24] 周清斌,龚文.浅谈土壤酶活性与土壤肥力的关系研究[J].中国农业信息,2014(9):104.
[25] FERNANDA D S T A,GODINHO S S H,TEOTONIO S D C,et al.Soil physicochemical properties and terrain information predict soil enzymes activity in phytophysiognomies of the Quadrilátero Ferrífero region in Brazil[J].Catena,2021,199:105083.
[26] 许留兴,李万荣,王晓亚,等.冬闲田混播小麦与豆科牧草对饲草产量、品质和土壤特性的影响[J].中国草地学报,2022,44(9):66-74.
[27] 李学博,李立军,马乃娇.油菜燕麦混播对饲草产量品质及土壤酶活性的影响[J].土壤通报,2020,51(4):897-904.
[28] 苟文龙,李平,马啸,等.混播比例和刈割茬次对一年生禾豆混播草地根系形态、土壤养分的影响[J].草原与草坪,2020,40(4):1-7.
[29] 范祎玮,信秀丽,钟新月,等.玉米豆科覆盖作物间作对潮土酶活性和真菌群落特征的影响[J].土壤,2021,53(6):1236-1243.
[30] 张丽琼,郝明德,臧逸飞,等.苜蓿和小麦长期连作对土壤酶活性及养分的影响[J].应用生态学报,2014,25(11):3191-3196.
[31] 郭川,赵艳兰,李香君,等.不同豆禾混播比例对植物种间关系及草地生产力的影响[J].草业科学,2022,39(11):2307-2316.
[32] 李扬,孙洪仁,丁宁,等.紫花苜蓿根系生物量[J].草地学报,2011,19(5):872-879.
[33] NIEMI R M,VEPSALAINEN M,WALLENIUS K,et al.Temporal and soil depth-related variation in soil enzyme activities and in root growth of red clover(Trifolium pratense) and timothy(Phleum pratense) in the field[J].Applied Soil Ecology,2005,30(2):113-125.
[34] 李发林,刘长全,傅金辉,等.土壤管理模式对幼龄果园根际土壤养分和酶活性影响初探[J].福建农业学报,2002(2):112-115.
[35] 杨凯,朱教君,张金鑫,等.不同林龄落叶松人工林土壤微生物生物量碳氮的季节变化[J].生态学报,2009,29(10):5500-5507.
[36] 宋伞伞,郑强卿,姚永生,等.不同产量水平下枣园土壤微生物量碳、氮与土壤和植株养分的关系[J].新疆农业科学,2020(10):1878-1891.
[37] 陈利云,汪之波,呼丽萍.6种豆科绿肥植物与苹果树套种对果园土壤碳氮特征的影响[J].草地学报,2021,29(4):671-676.
[38] 张莉,李友军,付国占,等.轮耕对冬小麦田土壤酶活性时空变化的影响[J].麦类作物学报,2014,34(8):1104-1110.
[39] 张锡洲,周建新,郑子成,等.水旱轮作条件下免耕土壤微生物生物量变化特征及其活性评价[J].四川农业大学学报,2010,28(2):153-158.
[40] 徐忠山,杨彦明,陈晓晶,等.菌肥对混播牧草土壤酶活性及微生物的影响[J].中国土壤与肥料,2018(6):77-83.
[41] 王玮. 燕麦与箭筈豌豆混播下燕麦性状表型和土壤养分及微生物群落结构的研究[D].呼和浩特:内蒙古农业大学,2023.
[42] 颜珲璘. 高寒地区禾/豆混播草地微生物与牧草互作机制研究[D].西宁:青海大学,2023.
[43] 张胜男,唐凯,高海燕,等.白桦林地土壤细菌群落特征及其驱动作用[J].森林工程,2024,40(2):27-35.
[44] 许春雪. 草地生态系统土壤微生物群落以及土壤代谢特征的研究[D].呼和浩特:内蒙古大学,2023.
[45] 王旭,曾昭海,朱波,等.燕麦与箭筈豌豆不同混作模式对根际土壤微生物数量的影响[J].草业学报,2009,18(6):151-157.

The effects of mixed sowing of hairy vetch and oat on crop yield,soil enzyme activity and microbial communities

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	HAN Xue, WANG Ying, ZHAO Xinyao, LI Xiaoting, ZHANG Yanli, YANG Jinhu, ZHOU Yang, LI Jiaqi, LI Lijun. Effect of nitrogen fertilizer on nitrogen balance index,photosynthetic characteristics and yield of wide-range densely planted oats [J]. Journal of Northern Agriculture, 2024, 52(1): 64-78.
[2]	LI Jiale, KONG Haoran, HE Xiaoshi, GUO Yuhang, NIU Jingheng, DONG Baozhu, BAO Haizhu, HAN Shengcai. Study on the control of oat anthracnose by Bacillus megaterium L6 [J]. Journal of Northern Agriculture, 2023, 51(3): 59-65.
[3]	ZHENG Chengzhong, XU Zhenpeng, ZHANG Zizhen, WANG Qianjun, MEI Xue, SUN Ying, WANG Fengwu, YE Lu. Comprehensive evaluation of agronomic,quality traits and adaptability of new naked oat varieties （lines） [J]. Journal of Northern Agriculture, 2023, 51(2): 12-21.
[4]	SUN Yueying, LIU Jinghui, MI Junzhen, ZHAO Baoping, LI Yinghao. The effect of lactobacillus compound preparation on the formation of oat population structure and root growth [J]. Journal of Northern Agriculture, 2023, 51(2): 33-40.
[5]	WANG Yaping, XU Linbo, WANG Lan, LIN Kejian, CUI Jin, ZHANG Quanyi. Identification of aphid resistance of different oat varieties and comprehensive evaluation of the production performance [J]. Journal of Northern Agriculture, 2022, 50(6): 72-79.
[6]	ZHANG Lu, ZHAI Xiaoyu, WU Junying, GAO Shihua, WANG Xuefeng, ZHAO Yufei. Evaluation on the regeneration capacity of different oat varieties after cutting [J]. Journal of Northern Agriculture, 2022, 50(4): 26-34.
[7]	BAI Jianhui, WANG Yuqing. Effects of exogenous spermine on photosynthetic characteristics of oat under alkali stress [J]. Journal of Northern Agriculture, 2022, 50(3): 9-16.
[8]	ZHANG Zhifen, FU Xiaofeng, YANG Haishun, FENG Baili, GAO Jinfeng, CUI Siyu, CHAI Yan, LIU Junqing. Evaluation of high yield and adaptability of naked oats（lines） [J]. Journal of Northern Agriculture, 2021, 49(5): 1-8.
[9]	HAN Yuting, CHEN Xiaojing, XU Zhongshan, LIU Jinghui, XUE Guoxing. The response of antioxidant system of different oat varieties to infection of stem rust [J]. Journal of Northern Agriculture, 2021, 49(4): 98-104.
[10]	YU Zhiguang, YAN Jie, MI Junzhen, ZHAO Baoping, LYU Pin, ZHANG Ru, WU Junying, LIU Jinghui. Effects of water and drought conditions on photosynthesis characteristics and assimilate accumulation on different oat varieties [J]. Journal of Northern Agriculture, 2021, 49(3): 22-28.
[11]	FENG Biao, Qinggeer, GAO Julin, YU Xiaofang, HU Shuping, ZHANG Xin, XUE Hongfei, LIU Ruizhi, LIU Aiye. Effects of different tillage methods on soil enzyme activity,microbial biomass and community composition [J]. Journal of Northern Agriculture, 2021, 49(3): 64-73.
[12]	ZHANG Tingting, WANG Lifang, ZHANG Dejian, LU Zhanyuan, CHENG Yuchen, LI Fu, LIU Zhanyong, DUN Huixia. Effect of different wheat straw return coverage on the diversity of soil microbial communities in dry farmland [J]. Journal of Northern Agriculture, 2021, 49(1): 77-87.
[13]	ZHANG Tingting, WANG Lifang, ZHANG Dejian, DUN Huixia. Effects of soil management measures and environmental factors on soil microorganisms in farmland [J]. Journal of Northern Agriculture, 2020, 48(6): 87-92.
[14]	ZHANG Jun, DUAN Yu, LIU Yahong, LIANG Junmei, WANG Bo, AN Hao, LIAN Haifei. Effects of different fertilization treatments on oat yield,nutrient absorption and water and fertilizer utilization efficiency [J]. Journal of Northern Agriculture, 2020, 48(5): 62-68.
[15]	WANG Lu, WANG Fengwu, GAO Qing, LIU Jinghui, ZHAO Baoping. Effect of different sowing date and combined application of nitrogen and phosphorus fertilizers on oats yield [J]. Journal of Northern Agriculture, 2020, 48(3): 80-85.