嫩江市大豆田反枝苋和藜对氟磺胺草醚的抗性

doi:10.12190/j.issn.2096-1197.2025.03.07

Abstract

Abstract: 【Objective】To determine the resistance levels and distribution of the broadleaf weeds Amaranthus retroflexus L. and Chenopodium album L. to fomesafen in soybean fields of Nenjiang City,Heilongjiang Province.【Methods】Seeds of A. retroflexus L. and C. album L. were collected from 10 towns,including Nenjiang Town,Haijiang Town,Qianjin Town etc. The resistance levels of weeds of A. retroflexus L. and C. album L. to fomesafen were determined using a whole-plant bioassay. The mechanisms underlying resistance in A. retroflexus L. were investigated through sequence amplification and alignment of the target gene PPX2 and other related methods.【Results】After treatment with different doses of fomesafen,the GR₅₀ values for 30 collected A. retroflexus L. populations ranged from 8.70 to 814.77 g a.i./hm²,with resistance indices（RI）of 17.8 to 93.6. Fourteen populations exhibited low-level resistance,primarily collected from Lianxing Township,Yilaha Town,and Taxi Township; ten populations showed moderate-level resistance,mainly from Shuangshan Town,Changfu Town,and Keluo Town; and two populations displayed high-level resistance,primarily from Haijiang Town. Target gene detection revealed that all 26 resistant A. retroflexus L. populations had an Arg-128Gly mutation in the PPX2 gene. A single resistant population of A. retroflexus L. R was selected and treated with P450 and GST inhibitors,resulting in a significant reduction of RI,indicating the presence of non-target-site resistance. After treatment with varying doses of fomesafen,the GR₅₀ values of 26 collected C. album L. populations ranged from 51.54 to 497.10 g a.i./hm²,with RI of 1.0 to 9.5. Ten populations exhibited low-level resistance,six showed moderate-level resistance,and no high-level resistant populations to fomesafen were identified. Resistant C. album L. populations to fomesafen were primarily collected from Linjiang Township,Changfu Town,and Keluo Town.【Conclusion】A. retroflexus L. and C. album L. in Nenjiang City,Heilongjiang Province,have developed different levels of resistance to fomesafen,with A. retroflexus L. exhibiting both target-site resistance and non-target-site resistance to fomesafen.

Key words: Nenjiang City, Soybean field, Amaranthus retroflexus L., Chenopodium album L., Fomesafen, Resistance

CLC Number:

S482.4

GUO Xiaotong, GUO Yulian, WANG Yu, LUO Chan, CONG Keqiang. Resistance of Amaranthus retroflexus L. and Chenopodium album L. to fomesafen in soybean fields of Nenjiang City[J].Journal of Northern Agriculture, 2025, 53(3): 60-75.

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Resistance of Amaranthus retroflexus L. and Chenopodium album L. to fomesafen in soybean fields of Nenjiang City

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