脱除SPVD甘薯组培苗快繁及移栽研究

doi:10.12190/j.issn.2096-1197.2021.05.16

Abstract

Abstract: 【Objective】 To screen the best hormone combination for rapid propagation of sweet potato virus-free tissue culture seedlings susceptible to sweet potato virus disease（SPVD）,and to provide technical support for the application of virus-free sweet potato seedlings in production. 【Methods】 Using Xushu 22 and Fuxushu 20 as test materials,the shoot apical meristem was cultured with varying detoxification times,virus inhibitors,combination of hormone concentration（NAA/6-BA）,subculture time and seedling cultivation. The removal rate of SPVD by different treatments was detected. The plant height of tissue cultured seedlings was measured. The survival rate of transplanting was counted. 【Results】 The removal rate of sweet potato chlorotic stunt virus（SPCSV）reached 100% in primary shoot apex meristem culture,and the removal rate of sweet potato feathery mottle virus（SPFMV）reached 100% in secondary shoot apex meristem culture. The removal rate of SPFMV stem tip pretreatment with virus inhibitor Ningnan·pyripeptide reached 100%. The optimal hormone combination for fast propagation of Xushu 22 tissue cultured seedlings was 0.05 mg/L NAA,1.0 mg/L 6-BA,and the optimal hormone combination for fast propagation of Fuxushu 20 tissue cultured seedlings was 0.01 mg/L NAA,1.0 mg/L 6-BA. The highest survival rate for Xushu 22 and Fuxushu 20 treated seedlings when subcultured for 60 days was 86.67% and 83.33%. 【Conclusion】 Secondary shoot apex meristem culture could completely eliminate SPVD. Virus inhibitor Ningnan·pyripeptide pretreatment increased the removal rate of SPFMV shoot apex. The optimal hormone combination for tissue culture seedlings for rapid propagation was 0.01-0.05 mg/L NAA,1.0 mg/L 6-BA. Refining seedlings increased the survival rate of transplanted tissue culture seedlings significantly.

Key words: Sweet potato, Sweet potato virus disease（SPVD）, Stem apex detoxification, Tissue culture, Rapid propagation, Transplant

CLC Number:

S531

YUAN Rui, TANG Wei, SUN Shujun, ZHAO Lukuan, HU Yang, MA Jukui, WANG Jie, CAO Qinghe, ZHOU Zhilin. Study on rapid propagation and transplanting of tissue culture seedlings of sweet potato with SPVD removed[J].Journal of Northern Agriculture, 2021, 49(5): 112-117.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2021.05.16

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Study on rapid propagation and transplanting of tissue culture seedlings of sweet potato with SPVD removed

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