Abstract: In order to stimulate students＇ interest in learning Freshwater Aquaculture course and improve the teaching effect, the teaching content, teaching methods and assessment methods of this course were explored and practiced. New advances and new technologies were introduced in the teaching content to stimulate the students＇ initiative. Multimedia and internet technology was comprehensively applied to enhance the teaching intuitiveness. The opening experiment content was set up and the innovation ability of students was enhanced. The assessment method was changed to inspire the learning motivation of students. Through the teaching reform of this course, the learning initiative and interest of students were enhanced, and the teaching quality was also improved.