猪A组轮状病毒VP7基因的克隆及生物信息学分析

doi:10.12160/j.issn.1672-5190.2020.01.003

畜牧与饲料科学 ›› 2020, Vol. 41 ›› Issue (1): 13-17.doi: 10.12160/j.issn.1672-5190.2020.01.003

猪A组轮状病毒VP7基因的克隆及生物信息学分析

谷长维¹, 谷鸣¹, 张玲¹, 胡博^1,2

1.中国农业科学院特产研究所,吉林长春 130112;
2.农业农村部经济动物疫病重点实验室,吉林长春 130112

收稿日期:2019-11-20 出版日期:2020-01-30 发布日期:2020-03-05
通讯作者: 胡博(1982—),男,副研究员,博士,主要从事兽用疫苗产品研制及开发工作。
作者简介:谷长维(1980—),男,医药工程师,硕士,主要从事分子病毒学与基因工程疫苗研究工作。

Cloning and Bioinformatical Analysis of VP7 Gene in Porcine Group A Rotavirus

GU Chang-wei¹, GU Ming¹, ZHANG Ling¹, HU Bo^1,2

1.Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
2.Key Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture and Rural Affairs, Changchun 130112, China

Received:2019-11-20 Online:2020-01-30 Published:2020-03-05

摘要/Abstract

摘要： 为了研究猪A组轮状病毒VP7基因功能,根据GenBank中猪轮状病毒VP7基因DNA序列设计引物,以实验室轮状病毒上海分离株SH-1为模板,进行PCR扩增,将VP7基因克隆到pMD-18T载体后,进行序列测定及分析。结果表明:此序列编码326个氨基酸,其相对分子量为37.38 Ku,理论等电点(PI)为4.77。VP7蛋白以α螺旋为主,主要有13个抗原表位区域。系统进化树分析显示分离株SH-1与KM230930.1株亲缘关系最近,基因型分析结果是G10型,属于人兽共患病毒株。

关键词: A组轮状病毒, VP7基因, 克隆, 生物信息学分析

Abstract: In order to investigate the function of porcine group A rotavirus VP7 gene, a set of primers were designed based on the DNA sequence of porcine rotavirus VP7 gene in GenBank. The rotavirus Shanghai isolate SH-1 was used as a template for PCR amplification, and the obtained VP7 gene was cloned into pMD-18T vector for sequencing and analysis. The results showed that the VP7 gene sequence encoded 326 amino acids with relative molecular weight of 37.38 Ku and theoretical isoelectric point (PI) of 4.77. The VP7 protein was dominated by alpha helices and contained 13 epitope regions. Phylogenetic tree analysis showed that the isolate SH-1 was genetically closest to KM230930.1 strain. The genotype analysis demonstrated that the isolate SH-1 was G10 type which belonged to the zoonotic virus strain.

Key words: group A rotavirus, VP7 gene, cloning, bioinformatical analysis

中图分类号:

S852.651

谷长维, 谷鸣, 张玲, 胡博. 猪A组轮状病毒VP7基因的克隆及生物信息学分析[J]. 畜牧与饲料科学, 2020, 41(1): 13-17.

GU Chang-wei, GU Ming, ZHANG Ling, HU Bo. Cloning and Bioinformatical Analysis of VP7 Gene in Porcine Group A Rotavirus[J]. Animal Husbandry and Feed Science, 2020, 41(1): 13-17.

参考文献

[1] World Health Organization.Diarrhoeal disease[EB/OL].(2017-05-02).http://www.who.int/mediacentre/factsheets/fs330/en/.
[2] World Health Organization.Rotavirus[EB/OL].(2018-12-01).http://www.who.int/immunization/diseases/rotavirus/en/.
[3] DIAN Z Q, FAN M, WANG B H,et al.The prevalence and genotype distribution of rotavirus A infection among children with acute gastroenteritis in Kunming,China[J].Archives of Virology,2017,162(1):281-285.
[4] GREENBERG H B,ESTES M K.Rotaviruses:from pathogenesis to vaccination[J].Gastroenterology,2009,136(6):1939-1951.
[5] THONGPRACHUM A,KHAMRIN P,MANEEKARN N,et al.Epidemiology of gastroenteritis viruses in Japan:prevalence,seasonality,and outbreak[J].Journal of Medical Virology,2016,88(4):551-570.
[6] ADAMS M J,HENDRICKSON R C,DEMPSEY D M,et al.Tracking the changes in virus taxonomy[J].Archives of Virology,2015,160(5):1375-1383.
[7] SOARES-WEISER K,MACLEHOSE H,BERGMAN H,et al.Vaccines for preventing rotavirus diarrhoea:vaccines in use[J].Cochrane Database of Systematic Reviews,2012(11):8521.
[8] CUI S H,TOBE R G,MO X T,et al.Cost-effectiveness analysis of rotavirus vaccination in China: projected possibility of scale-up from the current domestic option[J].BMC Infectious Diseases, 2016,16(1):677.
[9] KAWAI K,BRIEN M A,GOVEIA M G,et al.Burden of rotavirus gastroenteritis and distribution of rotavirus strains in Asia:a systematic review[J].Vaccine,2012,30(7):1244-1254.
[10] PAYNE D C,BAGGS J,ZERR D M,et al.Protective association between rotavirus and childhood seizures in the year following vaccination inUS children[J].Clinical Infectious Diseases,2014,58(2):173-177.
[11] PATEL M M,HABER P,BAGGS J,et al.Intussusception and rotavirus vaccination: a review of the available evidence[J].Expert Review of Vaccines,2009,8(11):1555-1564.

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猪A组轮状病毒VP7基因的克隆及生物信息学分析

Cloning and Bioinformatical Analysis of VP7 Gene in Porcine Group A Rotavirus

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